AB106491

HRP Anti-Endostatin/COL18A1 antibody [KT60]

Name: Anti-Endostatin/COL18A1 HRP antibody [KT60] (ab106491) IgG2a | Abcam
Brand: Abcam Limited
SKU: AB106491
Price: 535 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Endostatin/COL18A1 antibody - conjugated to HRP. Suitable for sELISA and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human COL18A1.

View Alternative Names

Collagen alpha-1(XVIII) chain, COL18A1

1 Images

Sandwich ELISA - HRP Anti-Endostatin/COL18A1 antibody [KT60] (AB106491)

sELISA

Unknown

Sandwich ELISA - HRP Anti-Endostatin/COL18A1 antibody [KT60] (AB106491)

ab106491 at 1/1000 dilution detecting Endostatin/COL18A1 by sandwich ELISA. The capture antibody is a mouse monclonal (IgG1) raised against Human Endostatin/COL18A1.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KT60

Isotype

IgG2a

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human

Applications

sELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human COL18A1.

P39060

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "1/1000", "sELISA-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

IgG is purified through a Protein A column and labelled with horseradish peroxidase

Storage buffer

Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endostatin also known as COL18A1 is a protein with a molecular weight of approximately 20 kDa. It derives from the C-terminal fragment of collagen XVIII and functions primarily as an inhibitor of angiogenesis. Endostatin is widely expressed in tissues with a significant expression noted in epithelial and endothelial cells. Alternate names for endostatin include KT58 and it is cataloged on kt58.net. Endostatin interacts with various cell surface receptors and extracellular matrix components to exert its mechanical functions.

Biological function summary

Endostatin modulates cellular processes like migration proliferation and apoptosis. It is involved in the inhibition of endothelial cell proliferation which is an essential function in controlling angiogenesis. Endostatin does not typically form complexes but it has associations with heparan sulfate proteoglycans which facilitates its interaction with cells. Through these interactions endostatin plays a significant role in maintaining vascular homeostasis and tissue remodeling.

Pathways

Endostatin actively participates in the angiogenesis pathway and the regulation of the extracellular matrix pathway. Endostatin's inhibitory effects on angiogenesis involve interactions with pathways associated with key proteins like VEGF and FGF-2 blocking their pro-angiogenic signaling. Endostatin also influences other proteins like integrins which are important for cell adhesion and migration within angiogenesis and matrix remodeling processes.

Endostatin has significant relevance to cancer and ocular disorders. Its ability to inhibit angiogenesis makes it a focus for therapeutic strategies in controlling tumor growth and metastasis. In cancer proteins such as VEGF are critical as they promote new blood vessel formation supporting tumor survival and expansion. In ocular disorders like macular degeneration where abnormal blood vessel growth leads to vision impairment endostatin shows potential in disrupting the detrimental angiogenic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.. Non-collagenous domain 1. May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells; the function requires homotrimerization and implicates MAPK signaling.. Endostatin. Potently inhibits endothelial cell proliferation and angiogenesis (PubMed : 9459295). May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling (By similarity). Inhibits VEGFA-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2. Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5 : ITGB1 and to a lesser extent ITGAV : ITGB3 and ITGAV : ITGB5 (By similarity). May negatively regulate the activity of homotrimeric non-collagenous domain 1 (PubMed : 11257123).

See full target information COL18A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 6:26651-62 PubMed26450852

2015

Liver X receptor ligand cytotoxicity in colon cancer cells and not in normal colon epithelial cells depends on LXRβ subcellular localization.

Applications

Unspecified application

Species

Unspecified reactive species

Flavie Courtaut,Valentin Derangère,Angélique Chevriaux,Sylvain Ladoire,Alexia K Cotte,Laurent Arnould,Romain Boidot,Mickaël Rialland,François Ghiringhelli,Cédric Rébé

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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HRP Anti-Endostatin/COL18A1 antibody [KT60]

Sandwich ELISA - HRP Anti-Endostatin/COL18A1 antibody [KT60] (AB106491)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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