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Rabbit Recombinant Monoclonal Ezrin antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - HRP Anti-Ezrin antibody [EP886Y] (AB198522), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Ezrin antibody [EP886Y] (AB198522), expandable thumbnail
  • Western blot - HRP Anti-Ezrin antibody [EP886Y] (AB198522), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

-

Associated Products

Select an associated product type

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2 products for Alternative Version

Target data

Function

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Alternative names

VIL2, EZR, Ezrin, Cytovillin, Villin-2, p81

Recommended products

Rabbit Recombinant Monoclonal Ezrin antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP886Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Ezrin also known as cytovillin or villin-2 is a protein belonging to the ezrin-radixin-moesin (ERM) family. It is approximately 81 kDa in molecular weight. Ezrin plays a critical role in linking the actin cytoskeleton to the plasma membrane. This interaction occurs through its ability to bind to membrane proteins and phospholipids facilitating structural support and signal transduction. Ezrin is widely expressed in epithelial tissues but it also occurs in other cell types such as lymphocytes and osteoclasts.

Biological function summary

Ezrin functions as a membrane-microfilament linker and is essential for maintaining cell shape adhesion and motility. It is an integral component of many cellular structures including microvilli and ruffles. Ezrin’s activity often involves forming complexes with other proteins such as EBP50 and F-actin which are fundamental for its role in cellular morphogenesis. These interactions allow ezrin to regulate membrane surface structure and cell surface organization.

Pathways

Ezrin participates in various signaling pathways that are essential for cell migration and invasion. One key pathway is the Rho family of GTPases which regulates actin cytoskeleton dynamics. Ezrin interacts with proteins like Rho-associated kinase (ROCK) that influence cell contraction and motility. Additionally ezrin is involved in the MAPK/ERK pathway which influences cell growth and survival by interacting with other signaling molecules.

Associated diseases and disorders

Ezrin shows significant relevance to cancer progression and metastasis particularly in conditions like osteosarcoma. Ezrin overexpression correlates with increased metastatic potential and poor clinical outcomes. It also has implications in allergic diseases due to its role in immune cell function. In these contexts ezrin interacts with SRC family kinases which are important for transmitting signals from cell-surface receptors to downstream pathways influencing disease development and progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - HRP Anti-Ezrin antibody [EP886Y] (ab198522), expandable thumbnail

    Western blot - HRP Anti-Ezrin antibody [EP886Y] (ab198522)

    ab198522 was shown to specifically react with Ezrin in wild-type HAP1 cells as signal was lost in EZR (Ezrin) knockout cells. Wild-type and EZR (Ezrin) knockout samples were subjected to SDS-PAGE. ab198522 and Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

    All lanes: Western blot - HRP Anti-Ezrin antibody [EP886Y] (ab198522) at 1/5000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: EZR (Ezrin) knockout HAP1 whole cell lysate at 20 µg

    Predicted band size: 69 kDa

    Observed band size: 80 kDa

    Exposure time: 1min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Ezrin antibody [EP886Y] (ab198522), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Ezrin antibody [EP886Y] (ab198522)

    IHC image of Ezrin staining in a section of formalin-fixed paraffin-embedded normal human lung*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198522 at 1/100 dilution for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-Ezrin antibody [EP886Y] (ab198522), expandable thumbnail

    Western blot - HRP Anti-Ezrin antibody [EP886Y] (ab198522)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198522 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - HRP Anti-Ezrin antibody [EP886Y] (ab198522) at 1/5000 dilution

    All lanes: Western blot - HeLa whole cell lysate (HeLa whole cell lysate ab150035) at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 69 kDa

    Observed band size: 80 kDa

    Exposure time: 18s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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