HRP Anti-Fatty Acid Synthase antibody [EPR7466]

• WB

Lab

Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (AB196854)

ab196854 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells as signal was lost in FASN (Fatty Acid Synthase) knockout cells. Wild-type and FASN (Fatty Acid Synthase) knockout samples were subjected to SDS-PAGE. ab196854 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor^® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

All lanes:

Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854) at 1/5000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

FASN (Fatty Acid Synthase) knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 273 kDa

Observed band size: 260 kDa

false

Exposure time: 1min

• WB

Lab

Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (AB196854)

This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196854 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (<a href='/en-us/products/cell-lysates/hela-whole-cell-lysate-ab150035'>ab150035</a>) at 10 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 3:

A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

Predicted band size: 273 kDa

Observed band size: 273 kDa

true

Exposure time: 8min