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AB314244

HRP Anti-Ferritin Heavy Chain antibody [EPR18878]

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Rabbit Recombinant Monoclonal Ferritin Heavy Chain antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.

View Alternative Names

Fth, Fth1, Ferritin heavy chain, Ferritin H subunit

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Ferritin Heavy Chain antibody [EPR18878] (AB314244)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Ferritin Heavy Chain antibody [EPR18878] (AB314244)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Ferritin Heavy Chain with ab314244 at 1/100 (5.0 µg/ml). Positive staining on mouse liver. The section was incubated with ab314244 at 4°C overnight. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Ferritin Heavy Chain antibody [EPR18878] (AB314244)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Ferritin Heavy Chain antibody [EPR18878] (AB314244)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Ferritin Heavy Chain with ab314244 at 1/100 (5.0 µg/ml). Positive staining on rat kidney. The section was incubated with ab314244 at 4°C overnight. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Ferritin Heavy Chain antibody [EPR18878] (AB314244)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Ferritin Heavy Chain antibody [EPR18878] (AB314244)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Ferritin Heavy Chain with ab314244 at 1/100 (5.0 µg/ml). Positive staining on rat liver. The section was incubated with ab314244 at 4°C overnight. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Western blot - HRP Anti-Ferritin Heavy Chain antibody [EPR18878] (AB314244)
  • WB

Supplier Data

Western blot - HRP Anti-Ferritin Heavy Chain antibody [EPR18878] (AB314244)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - HRP Anti-Ferritin Heavy Chain antibody [EPR18878] (ab314244) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Lane 3:

Rat kidney tissue lysate at 20 µg

Observed band size: 21 kDa

false

Exposure time: 180s

  • Unconjugated

    Anti-Ferritin Heavy Chain antibody [EPR18878]

  • Carrier free

    Anti-Ferritin Heavy Chain antibody [EPR18878] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18878

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: 68.9% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stores iron in a soluble, non-toxic, readily available form (By similarity). Important for iron homeostasis (By similarity). Has ferroxidase activity (By similarity). Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (By similarity). Also plays a role in delivery of iron to cells (PubMed : 19154717). Mediates iron uptake in capsule cells of the developing kidney (PubMed : 19154717). Degraded to release iron upon autophagy activation by nutrient starvation (PubMed : 25327288).
See full target information Fth1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com