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AB324031

HRP Anti-Firefly Luciferase antibody [EPR17789] - N-terminal

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Rabbit Recombinant Monoclonal LUCI antibody - conjugated to HRP. Suitable for WB and reacts with Transfected cell lysate - Firefly samples.

View Alternative Names

Luciferin 4-monooxygenase, Luciferase

1 Images
Western blot - HRP Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (AB324031)
  • WB

Supplier Data

Western blot - HRP Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (AB324031)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - HRP Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab324031) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a firefly Luciferase expression vector whole cell lysate at 20 µg

Observed band size: 61 kDa,36 kDa

false

Exposure time: 1s

  • 578 PE

    PE Anti-Firefly Luciferase antibody [EPR17789]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Firefly Luciferase antibody [EPR17789]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Firefly Luciferase antibody [EPR17789]

  • Unconjugated

    Anti-Firefly Luciferase antibody [EPR17789] - N-terminal

  • Carrier free

    Anti-Firefly Luciferase antibody [EPR17789] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17789

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB228530

Luciferase Reporter Assay Substrate Kit - Firefly

Functional Studies - Luciferase Reporter Assay Substrate Kit - Firefly (AB228530)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell lysate - Firefly": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Firefly luciferase also known as luc assay or luciferase protein is an enzyme that catalyzes the oxidation of luciferin resulting in bioluminescence. This protein has a molecular mass of approximately 61 kDa and is responsible for the characteristic glow of fireflies. The luciferase expression occurs mainly in the light-producing organs of fireflies such as in the insect Photinus pyralis where it plays an important role in light emission.
Biological function summary

Firefly luciferase converts chemical energy into light making it valuable for various biological applications as a reporter gene. It participates in the formation of a complex with its substrates luciferin and ATP resulting in the production of a photon. This enzyme is widely used in molecular and cellular biology experiments to monitor gene expression and study protein-protein interactions due to its luminescent properties.

Pathways

Firefly luciferase functions in the bioluminescent pathway involving the conversion of luciferin to oxyluciferin and light emission. It interfaces with ATP a universal energy carrier which highlights its integration into the cellular energy systems. Firefly luciferase is similar to other oxidative enzymes though it is not directly involved with metabolic pathways like the TCA cycle or glycolysis.

Researchers often utilize firefly luciferase as a tool in tumor studies or infectious disease models. Its luminescence aids in imaging and tracking biological processes in real-time. While firefly luciferase itself is not directly associated with specific diseases the use of firefly agents allows researchers to study disease outcomes assess therapeutic efficacy and understand gene expression profiles related to cancer and viral infections often in connection with proteins such as p53 in cancer research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Produces green light with a wavelength of 562 nm.
See full target information Luciferin 4-monooxygenase
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com