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Rabbit Monoclonal LUCI antibody - conjugated to HRP. Suitable for WB and reacts with Transfected cell lysate - Firefly samples.

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Images

Western blot - HRP Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (AB324031), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Transfected cell lysate - Firefly
Tested

Tested
Tested

Species
Transfected cell lysate - Firefly
Dilution info
1/1000
Notes

-

Target data

Function

Produces green light with a wavelength of 562 nm.

Alternative names

Luciferin 4-monooxygenase, Luciferase

Rabbit Monoclonal LUCI antibody - conjugated to HRP. Suitable for WB and reacts with Transfected cell lysate - Firefly samples.

Key facts

Isotype
IgG
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17789
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Firefly luciferase also known as luc assay or luciferase protein is an enzyme that catalyzes the oxidation of luciferin resulting in bioluminescence. This protein has a molecular mass of approximately 61 kDa and is responsible for the characteristic glow of fireflies. The luciferase expression occurs mainly in the light-producing organs of fireflies such as in the insect Photinus pyralis where it plays an important role in light emission.

Biological function summary

Firefly luciferase converts chemical energy into light making it valuable for various biological applications as a reporter gene. It participates in the formation of a complex with its substrates luciferin and ATP resulting in the production of a photon. This enzyme is widely used in molecular and cellular biology experiments to monitor gene expression and study protein-protein interactions due to its luminescent properties.

Pathways

Firefly luciferase functions in the bioluminescent pathway involving the conversion of luciferin to oxyluciferin and light emission. It interfaces with ATP a universal energy carrier which highlights its integration into the cellular energy systems. Firefly luciferase is similar to other oxidative enzymes though it is not directly involved with metabolic pathways like the TCA cycle or glycolysis.

Associated diseases and disorders

Researchers often utilize firefly luciferase as a tool in tumor studies or infectious disease models. Its luminescence aids in imaging and tracking biological processes in real-time. While firefly luciferase itself is not directly associated with specific diseases the use of firefly agents allows researchers to study disease outcomes assess therapeutic efficacy and understand gene expression profiles related to cancer and viral infections often in connection with proteins such as p53 in cancer research.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - HRP Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab324031), expandable thumbnail

    Western blot - HRP Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab324031)

    Firefly Luciferase Western blot staining using rabbit Anti-Firefly Luciferase antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) (1:200000) (36KDa).

    All lanes: Western blot - HRP Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab324031) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a firefly Luciferase expression vector whole cell lysate at 20 µg

    Observed band size: 61 kDa, 36 kDa

    Exposure time: 1s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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