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AB6656

HRP Anti-Fluorescein antibody

5

(2 Reviews)

|

(8 Publications)

Goat Polyclonal Fluorescein antibody - conjugated to HRP. Suitable for WB, ELISA, IHC-P, IHC-Fr and reacts with Chemical samples. Cited in 8 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Fluorescein.

View Alternative Names

FI, fluorescein isothyocianate, fluorescyl

2 Images
Immunohistochemistry (Frozen sections) - HRP Anti-Fluorescein antibody (AB6656)
  • IHC-Fr

AbReview14303****

Immunohistochemistry (Frozen sections) - HRP Anti-Fluorescein antibody (AB6656)

ab6656 staining GFP in Mouse mammary carcinoma metastasis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Perfusion fixed tissue with 4% paraformaldehyde, blocked with 0.5% Perkin-Elmer TNB blocking reagent for 30 minutes at 25°C and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500) for 30 minutes at 25°C

Micrograph demonstrates use of ab6662 for detection of GFP-positive tumor cells (LEFT) and an adjacent section after amplification with ab6656 (RIGHT). Images were taken with identical settings on a Leica confocal microscope.

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Fluorescein antibody (AB6656)
  • IHC-P

AbReview53209****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Fluorescein antibody (AB6656)

Immunohistochemical analysis of Mouse thymus in which the medullary epithellai cells are labelled with fluorescein-conjugated UEA1 lectin with ab6656. Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 40 minutes at 20°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 20°C.

This image is courtesy of an anonymous Abreview

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

IHC-P, WB, IHC-Fr, ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Fluorescein
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:19243 PubMed36357454

2022

Development of an anti-oxidative intraocular irrigating solution based on reactive persulfides.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroshi Kunikata,Hiroshi Tawarayama,Satoru Tsuda,Takaaki Akaike,Toru Nakazawa

Nucleic acids research 47:e61 PubMed30869144

2019

A panel of colorimetric assays to measure enzymatic activity in the base excision DNA repair pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Eleanor Healing,Clara F Charlier,Lisiane B Meira,Ruan M Elliott

The Plant cell 28:2616-2631 PubMed27650334

2016

Transcriptional Regulation of Arabidopsis Polycomb Repressive Complex 2 Coordinates Cell-Type Proliferation and Differentiation.

Applications

ISH

Species

Arabidopsis thaliana

Miguel de Lucas,Li Pu,Gina Turco,Allison Gaudinier,Ana Karina Morao,Hirofumi Harashima,Dahae Kim,Mily Ron,Keiko Sugimoto,Francois Roudier,Siobhan M Brady

BioMed research international 2016:4601532 PubMed27314021

2016

The Influence of Bone Marrow-Secreted IL-10 in a Mouse Model of Cerulein-Induced Pancreatic Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wey-Ran Lin,Siew-Na Lim,Tzung-Hai Yen,Malcolm R Alison

Methods in molecular biology (Clifton, N.J.) 1380:191-6 PubMed26552827

2015

Application of Aptamers in Histopathology.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Shigdar,Li Lv,Lifen Wang,Wei Duan

Theranostics 5:1083-97 PubMed26199647

2015

Superior Performance of Aptamer in Tumor Penetration over Antibody: Implication of Aptamer-Based Theranostics in Solid Tumors.

Applications

IHC

Species

Unspecified reactive species

Dongxi Xiang,Conglong Zheng,Shu-Feng Zhou,Shuxi Qiao,Phuong Ha-Lien Tran,Chunwen Pu,Yong Li,Lingxue Kong,Abbas Z Kouzani,Jia Lin,Ke Liu,Lianhong Li,Sarah Shigdar,Wei Duan

PloS one 8:e57613 PubMed23460885

2013

The use of sensitive chemical antibodies for diagnosis: detection of low levels of EpCAM in breast cancer.

Applications

IHC-P

Species

Human

Sarah Shigdar,Christine Qian,Li Lv,Chunwen Pu,Yong Li,Lianhong Li,Manju Marappan,Jia Lin,Lifen Wang,Wei Duan

Human molecular genetics 21:890-9 PubMed22080837

2011

LRRK2 Parkinson disease mutations enhance its microtubule association.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren R Kett,Daniela Boassa,Cherry Cheng-Ying Ho,Hardy J Rideout,Junru Hu,Masako Terada,Mark Ellisman,William T Dauer
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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