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AB193880

HRP Anti-FOXA2 antibody [EPR4466]

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(2 Publications )

Rabbit Recombinant Monoclonal FOXA2 antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications.
1 Images
Western blot - HRP Anti-FOXA2 antibody [EPR4466] (AB193880)
  • WB

Lab

Western blot - HRP Anti-FOXA2 antibody [EPR4466] (AB193880)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab193880 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-FOXA2 antibody [EPR4466] (ab193880) at 1/1000 dilution

All lanes:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 48 kDa

Observed band size: 52 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4466

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXA2 also known as HNF3B is a transcription factor that plays a significant role in gene regulation. This protein has a mass of approximately 47 kDa and is expressed in various tissues including the liver pancreas and lung. Researchers detect FOXA2 using techniques like ELISA for protein quantification. Its function involves regulating the expression of genes critical for development and metabolism.
Biological function summary

FOXA2 influences important processes in embryonic development and organogenesis. It does not typically act as part of a larger complex but independently binds to DNA enabling the transcription of target genes. FOXA2 affects liver function by regulating genes involved in glucose and lipid metabolism and it also plays a part in the differentiation of cells in development stages.

Pathways

FOXA2 operates within pathways like the insulin signaling pathway and the Wnt signaling pathway. It collaborates with other transcription factors like FOXA1 and FOXA3 to maintain metabolic homeostasis. In the Wnt pathway FOXA2 impacts cell fate decisions essential for embryonic patterning and development.

Alterations in FOXA2 expression have connections to conditions such as diabetes and liver diseases. Changes in FOXA2 expression could disrupt liver metabolism leading to metabolic disorders. Moreover FOXA2 ties to the regulation of other proteins like PDX1 in the context of diabetes where it plays a role in insulin biosynthesis and pancreatic cell function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
See full target information FOXA2

Alternative Names

HNF3B, TCF3B, FOXA2, Hepatocyte nuclear factor 3-beta, HNF-3-beta, HNF-3B, Forkhead box protein A2, Transcription factor 3B, TCF-3B
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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

Nature communications 15:7860 PubMed39251590

2024

Identification of an embryonic differentiation stage marked by Sox1 and FoxA2 co-expression using combined cell tracking and high dimensional protein imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Geethika Arekatla,Stavroula Skylaki,David Corredor Suarez,Hartland Jackson,Denis Schapiro,Stefanie Engler,Markus Auler,German Camargo Ortega,Simon Hastreiter,Andreas Reimann,Dirk Loeffler,Bernd Bodenmiller,Timm Schroeder

APL bioengineering 5:026104 PubMed34027283

2021

Investigation of the hepatic development in the coculture of hiPSCs-derived LSECs and HLCs in a fluidic microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Mathieu Danoy,Yannick Tauran,Stephane Poulain,Rachid Jellali,Johanna Bruce,Marjorie Leduc,Morgane Le Gall,Yuta Koui,Hiroshi Arakawa,Francoise Gilard,Bertrand Gakiere,Yukio Kato,Charles Plessy,Taketomo Kido,Atsushi Miyajima,Yasuyuki Sakai,Eric Leclerc
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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