JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB199457

HRP Anti-G-CSF antibody [EPR3203(N)(B)]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal G-CSF antibody - conjugated to HRP. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin

1 Images
Western blot - HRP Anti-G-CSF antibody [EPR3203(N)(B)] (AB199457)
  • WB

Lab

Western blot - HRP Anti-G-CSF antibody [EPR3203(N)(B)] (AB199457)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199457 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-G-CSF antibody [EPR3203(N)(B)] (ab199457) at 1/5000 dilution

All lanes:

K-562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate (ab76827) at 10 µg

Predicted band size: 22 kDa

Observed band size: 26 kDa

true

Exposure time: 30s

  • Carrier free

    Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free

  • Carrier free

    Anti-G-CSF antibody [EPR3203(N)(B)] - Low endotoxin, Azide free

  • Unconjugated

    Anti-G-CSF antibody [EPR3203(N)(B)]

  • 660 APC

    APC Anti-G-CSF antibody [EPR3203(N)(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-G-CSF antibody [EPR3203(N)(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-G-CSF antibody [EPR3203(N)(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-G-CSF antibody [EPR3203(N)(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-G-CSF antibody [EPR3203(N)(B)]

  • 578 PE

    PE Anti-G-CSF antibody [EPR3203(N)(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3203(N)(B)

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB9691

Anti-G-CSF antibody

Sandwich ELISA - Anti-G-CSF antibody (AB9691)

  • 4
  • 6
Primary Antibodies

AB216607

Anti-G-CSF antibody [CSF3/900]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody [CSF3/900] (AB216607)

  • 0
  • 0
Primary Antibodies

AB270267

Anti-G-CSF antibody [CSF3/3166R]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody [CSF3/3166R] (AB270267)

  • 0
  • 0
Primary Antibodies

AB212315

Anti-G-CSF antibody [CSF3/900] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody [CSF3/900] - BSA and Azide free (AB212315)

  • 0
  • 0
Primary Antibodies

AB204989

Anti-G-CSF antibody [7E4F7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody [7E4F7] (AB204989)

  • 0
  • 0
Primary Antibodies

AB197993

Anti-G-CSF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody (AB197993)

  • 0
  • 0
Primary Antibodies

AB231277

Anti-G-CSF antibody

Western blot - Anti-G-CSF antibody (AB231277)

  • 0
  • 0
Primary Antibodies

AB212316

Anti-G-CSF antibody [SPM468] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody [SPM468] - BSA and Azide free (AB212316)

  • 0
  • 0
Primary Antibodies

AB110652

Anti-G-CSF antibody [KT26]

Sandwich ELISA - Anti-G-CSF antibody [KT26] (AB110652)

  • 0
  • 0

Complementary Products

Assay Kits

AB264627

Human G-CSF ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human G-CSF ELISA Kit (AB264627)

  • 0
  • 0
Assay Kits

AB264627

Human G-CSF ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human G-CSF ELISA Kit (AB264627)

  • 0
  • 0
Primary Antibodies

AB272359

Anti-EPO antibody [EPR22931-29] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-EPO antibody [EPR22931-29] - BSA and Azide free (Detector) (AB272359)

  • 0
  • 0
Primary Antibodies

AB307164

Anti-TNF alpha antibody [RM1005]

BOND RX™ Validated|Recombinant|Lab Essentials|20ul selling size|RabMAb

Flow Cytometry (Intracellular) - Anti-TNF alpha antibody [RM1005] (AB307164)

  • 0
  • 0
Primary Antibodies

AB207327

Anti-Albumin antibody [EPR20195]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (AB207327)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
See full target information CSF3
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com