AB196325

HRP Anti-GAP43 antibody [EP890Y] - Neuronal Marker

BOND RX™ Validated
RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal GAP43 antibody - conjugated to HRP. Neuron marker. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Neuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, pp46, GAP43

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAP43 antibody [EP890Y] - Neuronal Marker (AB196325)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAP43 antibody [EP890Y] - Neuronal Marker (AB196325)

IHC image of GAP43 staining in a section of formalin-fixed paraffin-embedded normal human cerebellum, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196325 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

578 PE

PE Anti-GAP43 antibody [EP890Y] - Neuronal Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-GAP43 antibody [EP890Y] - Neuronal Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-GAP43 antibody [EP890Y] - Neuronal Marker
Unconjugated

Anti-GAP43 antibody [EP890Y] - Neuronal Marker
Carrier free

Anti-GAP43 antibody [EP890Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP890Y

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GAP43 commonly called Growth Associated Protein 43 or neuronal marker is a protein with a molecular weight of approximately 25-50 kDa. Researchers identify it by various names including GAP-43 GAP43 anticuerpo or GAP43 neuronal marker. This protein exhibits a specific presence in the nervous system especially in axons of neurons. It has significant expression during neural development and regeneration indicating its role in growth and repair.

Biological function summary

GAP43 plays an important role in neurite outgrowth and synaptic plasticity. It functions as an important mediator of signal transduction in neurons. GAP43 does not act alone but integrates into a network of signaling pathways that guide axonal elongation and branching. It works closely with cytoskeletal elements influencing the structural dynamics needed for effective neuronal response and adaptation.

Pathways

GAP43 is vital in the regulation of the protein kinase C pathway and the cAMP pathway both essential for neuronal growth and differentiation. It closely interacts with proteins such as calmodulin which binds to GAP43 and impacts its function in those pathways. These interactions highlight its role in the modulation of neuronal growth and synaptic plasticity making it essential for neurons' response to environmental signals.

GAP43 associates with neurodegenerative diseases and cognitive disorders particularly Alzheimer's disease and schizophrenia. Changes in GAP43 expression levels correlate with the severity and progression of these conditions. Additionally GAP43 connects to other proteins implicated in such diseases like tau in Alzheimer's suggesting a multifaceted involvement in disease mechanisms. This makes it a target of interest for therapeutic research as modulating GAP43 activity might alter disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.

See full target information GAP43

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neurology 11:586958 PubMed33584493

2021

Operation Brain Trauma Therapy: An Exploratory Study of Levetiracetam Treatment Following Mild Traumatic Brain Injury in the Micro Pig.

Applications

Unspecified application

Species

Unspecified reactive species

Audrey Lafrenaye,Stefania Mondello,John Povlishock,Karen Gorse,Susan Walker,Ronald Hayes,Kevin Wang,Patrick M Kochanek

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-GAP43 antibody [EP890Y] - Neuronal Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAP43 antibody [EP890Y] - Neuronal Marker (AB196325)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise