AB204481

HRP Anti-GAPDH antibody [EPR16884] - Loading Control

BOND RX™ Validated
RabMAb
Recombinant
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(8 Publications)

Rabbit Recombinant Monoclonal GAPDH antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

View Alternative Names

GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

2 Images

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAPDH antibody [EPR16884] - Loading Control (AB204481)

IHC image of GAPDH staining in a section of formalin-fixed paraffin-embedded normal human skeletal muscle*, performed on a Leica BOND^TM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab204481, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Lab

Western blot - HRP Anti-GAPDH antibody [EPR16884] - Loading Control (AB204481)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab204481 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-GAPDH antibody [EPR16884] - Loading Control (ab204481) at 1/5000 dilution

Lane 1:

Brain (Human) Tissue Lysate - fetal normal tissue at 20 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 3:

Brain (Rat) Tissue Lysate at 10 µg

Lane 4:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 5:

NIH 3T3 (Mouse) Whole Cell Lysate at 20 µg

Lane 6:

C6 (Rat glioma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 36 kDa

Observed band size: 36 kDa

true

Exposure time: 4s

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-GAPDH antibody [EPR16884]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-GAPDH antibody [EPR16884]
578 PE

PE Anti-GAPDH antibody [EPR16884]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-GAPDH antibody [EPR16884]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16884

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.

Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed : 11724794, PubMed : 3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed : 11724794, PubMed : 3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed : 23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed : 23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed : 23332158, PubMed : 27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).

See full target information GAPDH

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109646 PubMed38638566

2024

Frequent loss of expression in colorectal cancer results in selective dependency.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Li,Ting Han

Nature cell biology 26:593-603 PubMed38553595

2024

Cyclophilin A supports translation of intrinsically disordered proteins and affects haematopoietic stem cell ageing.

Applications

Unspecified application

Species

Unspecified reactive species

Laure Maneix,Polina Iakova,Charles G Lee,Shannon E Moree,Xuan Lu,Gandhar K Datar,Cedric T Hill,Eric Spooner,Jordon C K King,David B Sykes,Borja Saez,Bruno Di Stefano,Xi Chen,Daniela S Krause,Ergun Sahin,Francis T F Tsai,Margaret A Goodell,Bradford C Berk,David T Scadden,André Catic

Oncology research 29:251-262 PubMed37303940

2023

Long noncoding RNA PPP1R14B-AS1 imitates microRNA-134-3p to facilitate breast cancer progression by upregulating LIM and SH3 protein 1.

Applications

Unspecified application

Species

Unspecified reactive species

Limin Zhou,Lianbo Zhang,Xin Guan,Y I Dong,Tao Liu

Cancer research communications 2:1693-1710 PubMed36846090

2022

Proteasome Inhibitors Silence Oncogenes in Multiple Myeloma through Localized Histone Deacetylase 3 (HDAC3) Stabilization and Chromatin Condensation.

Applications

Unspecified application

Species

Unspecified reactive species

Laure Maneix,Polina Iakova,Shannon E Moree,Joanne I Hsu,Ragini M Mistry,Fabio Stossi,Premal Lulla,Zheng Sun,Ergun Sahin,Sarvari V Yellapragada,André Catic

Scientific reports 10:13942 PubMed32811853

2020

The ubiquitin ligase Cullin-1 associates with chromatin and regulates transcription of specific c-MYC target genes.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie A Sweeney,Polina Iakova,Laure Maneix,Fu-Yuan Shih,Hannah E Cho,Ergun Sahin,Andre Catic

International journal of molecular medicine 46:119-130 PubMed32319550

2020

Long non‑coding RNA PRNCR1 exerts oncogenic effects in tongue squamous cell carcinoma in vitro and in vivo by sponging microRNA‑944 and thereby increasing HOXB5 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Lin,Yanan Zou,Ruijing Li,Daofeng Liu

Molecular medicine reports 19:221-230 PubMed30483781

2018

Rapamycin‑induced autophagy attenuates hormone‑imbalance‑induced chronic non‑bacterial prostatitis in rats via the inhibition of NLRP3 inflammasome‑mediated inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jingxiao Lu,Yang Su,Xianguo Chen,Yuan Chen,Pengcheng Luo,Fangyou Lin,Jie Zhang

Experimental and therapeutic medicine 14:2329-2334 PubMed28962163

2017

Roles of miR-138 and ISG15 in oral squamous cell carcinoma.

Applications

Species

Unspecified reactive species

Qimei Zhang,Yi He,Minhai Nie,Wei Cai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-GAPDH antibody [EPR16884] - Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAPDH antibody [EPR16884] - Loading Control (AB204481)

Western blot - HRP Anti-GAPDH antibody [EPR16884] - Loading Control (AB204481)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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