Rabbit Recombinant Monoclonal GAPDH antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 11 publications.
Images
![Western blot - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (AB201822), expandable thumbnail](https://content.abcam.com/products/images/hrp-gapdh-antibody-epr16891-loading-control-ab201822--western-blot-img51281.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (AB201822)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (AB201822), expandable thumbnail](https://content.abcam.com/products/images/hrp-gapdh-antibody-epr16891-loading-control-ab201822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77199.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (AB201822)")
Publications
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- International journal of molecular sciences 24:2023Thymoquinone Inhibition of Chemokines in TNF-α-Induced Inflammatory and Metastatic Effects in Triple-Negative Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGetinet M Adinew,Samia Messeha,Equar Taka,Bereket Mochona,Kinfe K Redda,Karam F A SolimanPubMed 37373025
- Frontiers in molecular neuroscience 15:10250662023Discovery of an evodiamine derivative for PI3K/AKT/GSK3β pathway activation and AD pathology improvement in mouse models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuo Pang,Siyuan Li,Hanzeng Cheng,Zhuohui Luo,Xiaolong Qi,Feifei Guan,Wei Dong,Shan Gao,Ning Liu,Xiang Gao,Shuo Pan,Xu Zhang,Li Zhang,Yajun Yang,Lianfeng ZhangPubMed 36698780
- Life science alliance 6:2023Integrin β1/FAK/SRC signal pathway is involved in autism spectrum disorder in knockout rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuo Pang,Zhuohui Luo,Wei Dong,Shan Gao,Wei Chen,Ning Liu,Xu Zhang,Xiang Gao,Jing Li,Kai Gao,Xudong Shi,Feifei Guan,Li Zhang,Lianfeng ZhangPubMed 36625203
- Drug design, development and therapy 16:3285-32962022Synthesis and Bioactivity Evaluation of a Novel 1,2,4-Oxadiazole Derivative in vitro and in 3×Tg Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuohui Luo,Yongcheng Wang,Shuo Pang,Shan Gao,Ning Liu,Xiang Gao,Li Zhang,Xiaolong Qi,Yajun Yang,Lianfeng ZhangPubMed 36187086
- Animal models and experimental medicine 4:381-3902021Novel rat model of multiple mitochondrial dysfunction syndromes (MMDS) complicated with cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYahao Ling,Jiaxin Ma,Xiaolong Qi,Xu Zhang,Qi Kong,Feifei Guan,Wei Dong,Wei Chen,Shan Gao,Xiang Gao,Shuo Pan,Yuanwu Ma,Dan Lu,Lianfeng ZhangPubMed 34977489
- Redox biology 46:1020942021Cell cycle induction in human cardiomyocytes is dependent on biosynthetic pathway activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRiham R E Abouleisa,Lindsey McNally,Abou Bakr M Salama,Sally K Hammad,Qinghui Ou,Collin Wells,Pawel K Lorkiewicz,Roberto Bolli,Tamer M A Mohamed,Bradford G HillPubMed 34418597
- Antioxidants (Basel, Switzerland) 10:2021Antioxidant Effect of L. in the Cardiovascular System of Obese Zucker Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEzgi Dayar,Martina Cebova,Jan Lietava,Elena Panghyova,Olga PechanovaPubMed 34439452
- Alzheimer's research & therapy 13:322021Erythropoietin-derived peptide treatment reduced neurological deficit and neuropathological changes in a mouse model of tauopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun-Beom Choi et. al.PubMed 33504364
- Molecular medicine reports 19:1903-19102019MicroRNA‑744 suppresses cell proliferation and invasion of papillary thyroid cancer by directly targeting NOB1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaixia Liu et. al.PubMed 30628685
- American journal of cancer research 8:2238-22532018microRNA-744 is downregulated in glioblastoma and inhibits the aggressive behaviors by directly targeting NOB1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifan Deng et. al.PubMed 30555741
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
| Chicken | Predicted | Predicted |
| Fish | Predicted | Predicted |
| Monkey | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Fish, Monkey, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 | Notes - |
Species Rat | Dilution info 1/5000 | Notes - |
Species Human | Dilution info 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Fish, Monkey, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
7 products for Alternative Version
Target data
Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
Alternative names
Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, OK/SW-cl.12, CDABP0047, GAPD, GAPDH
Recommended products
Rabbit Recombinant Monoclonal GAPDH antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 11 publications.
Alternative names
Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, OK/SW-cl.12, CDABP0047, GAPD, GAPDH
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR16891
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
- Biological function summary
GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.
- Pathways
GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.
- Associated diseases and disorders
GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822), expandable thumbnail](https://content.abcam.com/products/images/hrp-gapdh-antibody-epr16891-loading-control-ab201822--western-blot-img51281.jpg "Western blot - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)")
Western blot - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201822 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822) at 1/5000 dilution
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 4: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 5: Brain (Mouse) Tissue Lysate at 10 µg
Lane 6: Brain (Rat) Tissue Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 30s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822), expandable thumbnail](https://content.abcam.com/products/images/hrp-gapdh-antibody-epr16891-loading-control-ab201822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77199.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)
IHC image of GAPDH staining in a section of formalin-fixed paraffin-embedded human kidney renal cell carcinoma tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab201822 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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