AB201822

HRP Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb
Recombinant
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(18 Publications)

Rabbit Recombinant Monoclonal GAPDH antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 18 publications.

View Alternative Names

GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

2 Images

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (AB201822)

IHC image of GAPDH staining in a section of formalin-fixed paraffin-embedded human kidney renal cell carcinoma tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab201822 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Lab

Western blot - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (AB201822)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201822 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822) at 1/5000 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 5:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 6:

Brain (Rat) Tissue Lysate at 10 µg

Predicted band size: 36 kDa

Observed band size: 36 kDa

true

Exposure time: 30s

421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-GAPDH antibody [EPR16891]
578 PE

PE Anti-GAPDH antibody [EPR16891]
519 FITC

FITC Anti-GAPDH antibody [EPR16891]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-GAPDH antibody [EPR16891]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-GAPDH antibody [EPR16891]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-GAPDH antibody [EPR16891]
Unconjugated

Anti-GAPDH antibody [EPR16891] - Loading Control
Carrier free

Anti-GAPDH antibody [EPR16891] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16891

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Mouse, Rat, Human, Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Fish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.

Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis (PubMed : 11724794, PubMed : 3170585). Also shows nitrosylase activity, thereby playing a role in nuclear functions (PubMed : 11724794, PubMed : 3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed : 23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed : 23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed : 23332158, PubMed : 27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).

See full target information GAPDH

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:227 PubMed40114208

2025

Reducing severity of inflammatory bowel disease through colonization of Lactiplantibacillus plantarum and its extracellular vesicles release.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Wu,Xinyue Huang,Qianbei Li,Chaoqun Yang,Xixin Huang,Hualongyue Du,Bo Situ,Lei Zheng,Zihao Ou

Pathophysiology : the official journal of the International Society for Pathophysiology 32: PubMed39982361

2025

Evolocumab Reduces Oxidative Stress and Lipid Peroxidation in Obese Zucker Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Cebova,Radoslava Bulkova,Olga Pechanova

Oncology letters 27:262 PubMed38646496

2024

MicroRNA‑1224 inhibits cell proliferation by downregulating CBX3 expression in chordoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Xia,Jihe Huang,Chunhua Sun,Fei Shen,Kejia Yang

International journal of molecular sciences 25: PubMed38255922

2024

Aliskiren-Loaded Nanoparticles Downregulate (Pro)renin Receptor and ACE Gene Expression in the Heart of Spontaneously Hypertensive Rats: Effect on NADPH Oxidase.

Applications

Unspecified application

Species

Unspecified reactive species

Andrej Barta,Martina Cebova,Andrej Kovac,Martina Koneracka,Vlasta Zavisova,Olga Pechanova

International journal of molecular sciences 24: PubMed37373025

2023

Thymoquinone Inhibition of Chemokines in TNF-α-Induced Inflammatory and Metastatic Effects in Triple-Negative Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Getinet M Adinew,Samia Messeha,Equar Taka,Bereket Mochona,Kinfe K Redda,Karam F A Soliman

Allergologia et immunopathologia 51:191-204 PubMed36916106

2023

SIRT3 improves alveolar epithelial cell damage caused by bronchopulmonary dysplasia through deacetylation of FOXO1.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Zang,Jinghan Chi,Sitong Bi,Yi Tao,Rong Wang,Lihua Li

Frontiers in molecular neuroscience 15:1025066 PubMed36698780

2023

Discovery of an evodiamine derivative for PI3K/AKT/GSK3β pathway activation and AD pathology improvement in mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Pang,Siyuan Li,Hanzeng Cheng,Zhuohui Luo,Xiaolong Qi,Feifei Guan,Wei Dong,Shan Gao,Ning Liu,Xiang Gao,Shuo Pan,Xu Zhang,Li Zhang,Yajun Yang,Lianfeng Zhang

Life science alliance 6: PubMed36625203

2023

Integrin β1/FAK/SRC signal pathway is involved in autism spectrum disorder in knockout rats.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Pang,Zhuohui Luo,Wei Dong,Shan Gao,Wei Chen,Ning Liu,Xu Zhang,Xiang Gao,Jing Li,Kai Gao,Xudong Shi,Feifei Guan,Li Zhang,Lianfeng Zhang

International journal of molecular sciences 24: PubMed36613727

2022

Combined Therapy with Simvastatin- and Coenzyme-Q10-Loaded Nanoparticles Upregulates the Akt-eNOS Pathway in Experimental Metabolic Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Ezgi Şaman,Martina Cebova,Andrej Barta,Martina Koneracka,Vlasta Zavisova,Anita Eckstein-Andicsova,Martin Danko,Jaroslav Mosnacek,Olga Pechanova

Drug design, development and therapy 16:3285-3296 PubMed36187086

2022

Synthesis and Bioactivity Evaluation of a Novel 1,2,4-Oxadiazole Derivative in vitro and in 3×Tg Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuohui Luo,Yongcheng Wang,Shuo Pang,Shan Gao,Ning Liu,Xiang Gao,Li Zhang,Xiaolong Qi,Yajun Yang,Lianfeng Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-GAPDH antibody [EPR16891] - Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (AB201822)

Western blot - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (AB201822)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise