AB185059

HRP Anti-GAPDH antibody [EPR6256] - Loading Control

Name: Anti-GAPDH - Loading Control HRP antibody [EPR6256] (ab185059) Rabbit monoclonal IgG | Abcam
Brand: Abcam Limited
SKU: AB185059
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(18 Publications)

Rabbit Recombinant Monoclonal GAPDH antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human samples. Cited in 18 publications.

View Alternative Names

GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

1 Images

Unknown

Western blot - HRP Anti-GAPDH antibody [EPR6256] - Loading Control (AB185059)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab185059 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406

All lanes:

Western blot - HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059) at 1/5000 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 3:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Predicted band size: 36 kDa

Observed band size: 38 kDa

true

Exposure time: 2s

Carrier free

Anti-GAPDH antibody [EPR6256] - BSA and Azide free
Unconjugated

Anti-GAPDH antibody [EPR6256] - Loading Control
660 APC

APC Anti-GAPDH antibody [EPR6256]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-GAPDH antibody [EPR6256]
Biotin

Biotin Anti-GAPDH antibody [EPR6256] - Loading Control
675 PerCP

PerCP Anti-GAPDH antibody [EPR6256]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6256

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.

Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis (PubMed : 11724794, PubMed : 3170585). Also shows nitrosylase activity, thereby playing a role in nuclear functions (PubMed : 11724794, PubMed : 3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed : 23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed : 23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed : 23332158, PubMed : 27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).

See full target information GAPDH

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

ACS infectious diseases 10:3853-3867 PubMed39475219

2024

Modulators of the Hop-HSP90 Protein-Protein Interaction Disrupt KSHV Lytic Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Michael O Okpara,Michaelone C Vaaltyn,Jessica L Watson,Mahama Alhassan,Fernando Albericio,Beatriz G de la Torre,David J Clarke,Clinton G L Veale,Adrienne L Edkins

Npj gut and liver 1:7 PubMed39381160

2024

Stiffness-induced modulation of ERG transcription factor in chronic liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia-Emilia Selicean,Eric Felli,Cong Wang,Yeldos Nulan,Juan José Lozano,Sergi Guixé-Muntet,Horia Ștefănescu,Jaime Bosch,Annalisa Berzigotti,Jordi Gracia-Sancho

Antiviral research 230:105990 PubMed39154751

2024

Discovery of a small-molecule inhibitor of KSHV lytic replication from the MMV pandemic response box.

Applications

Unspecified application

Species

Unspecified reactive species

Michael O Okpara,Frederick Weaver,Adrian Whitehouse,Clinton G L Veale,Adrienne L Edkins

Molecular medicine reports 29: PubMed38063230

2023

Overexpression of salusin‑β downregulates adipoR1 expression to prevent fatty acid oxidation in HepG2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aohong Xu,Lei Wang,Min Luo,Huan Zhang,Meiwei Ning,Jintong Pan,Xiuqun Duan,Yuxue Wang,Xiang Liu

Nature 617:154-161 PubMed37100900

2023

Alternative CDC20 translational isoforms tune mitotic arrest duration.

Applications

Unspecified application

Species

Unspecified reactive species

Mary-Jane Tsang,Iain M Cheeseman

Cell 185:4634-4653.e22 PubMed36347254

2022

The phenotypic landscape of essential human genes.

Applications

Unspecified application

Species

Unspecified reactive species

Luke Funk,Kuan-Chung Su,Jimmy Ly,David Feldman,Avtar Singh,Brittania Moodie,Paul C Blainey,Iain M Cheeseman

Molecular and clinical oncology 14:94 PubMed33767863

2021

IP6K2 predicts favorable clinical outcome of primary breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Josefine Sandström,Alien Balian,Rebecca Lockowandt,Tommy Fornander,Bo Nordenskjöld,Linda Lindström,Gizeh Pérez-Tenorio,Olle Stål

Cancer biotherapy & radiopharmaceuticals : PubMed32818383

2020

lncRNA DANCR Promotes Proliferation and Metastasis of Breast Cancer Cells Through Sponging miR-4319 and Upregulating VAPB.

Applications

Unspecified application

Species

Unspecified reactive species

Haiquan Jia,Kai Liang,Guohua Liu,Zeshuai Zhang,Yuan Shi,Hao Liang,Peng Liu

International journal of molecular sciences 21: PubMed32365744

2020

STIP1/HOP Regulates the Actin Cytoskeleton through Interactions with Actin and Changes in Actin-Binding Proteins Cofilin and Profilin.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha Joy Beckley,Morgan Campbell Hunter,Sarah Naulikha Kituyi,Ianthe Wingate,Abantika Chakraborty,Kelly Schwarz,Matodzi Portia Makhubu,Robert Pierre Rousseau,Duncan Kyle Ruck,Jo-Anne de la Mare,Gregory Lloyd Blatch,Adrienne Lesley Edkins

Molecular cell 78:824-834.e15 PubMed32325029

2020

Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Schumann,Stacy Alyse Malaker,Simon Peter Wisnovsky,Marjoke Froukje Debets,Anthony John Agbay,Daniel Fernandez,Lauren Jan Sarbo Wagner,Liang Lin,Zhen Li,Junwon Choi,Douglas Michael Fox,Jessie Peh,Melissa Anne Gray,Kayvon Pedram,Jennifer Jean Kohler,Milan Mrksich,Carolyn Ruth Bertozzi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-GAPDH antibody [EPR6256] - Loading Control

Western blot - HRP Anti-GAPDH antibody [EPR6256] - Loading Control (AB185059)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise