AB9385

HRP Anti-GAPDH antibody - Loading Control

Name: Anti-GAPDH - Loading Control HRP antibody (ab9385) IgG | Abcam
Brand: Abcam Limited
SKU: AB9385
Price: 490 USD
Availability: InStock
Rating: 4 (11 reviews)

(11 Reviews)

(106 Publications)

Rabbit Polyclonal GAPDH antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 106 publications.

View Alternative Names

GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

2 Images

Unknown

Western blot - HRP Anti-GAPDH antibody - Loading Control (AB9385)

Lane 1 - 4 : GAPDH antibody - Loading Control (ab9385) at 1 ug/ml

Lane 1 : HeLa cell lysate at 20 ug
Lane 2 : A431 cell lysate at 20 ug
Lane 3 : Jurkat cell lysate at 20 ug
Lane 4 : 293 cell lysate at 20 ug

Performed under reducing conditions.

Exposure time : 1 minute

All lanes:

Western blot - HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1 µg/mL

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

293 cell lysate at 20 µg

Predicted band size: 36 kDa

Observed band size: 38 kDa

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Exposure time: 1min

Ap754275****

Western blot - HRP Anti-GAPDH antibody - Loading Control (AB9385)

The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.

All lanes:

Western blot - HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1/5000 dilution

Lanes 1 and 5:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 10 µg

Lanes 2 and 6:

Jurkat (Human) Whole Cell Lysate (ab52254) at 10 µg

Lanes 3 and 7:

Western blot - A-431 whole cell lysate (<a href='/en-us/products/cell-lysates/a-431-whole-cell-lysate-ab7909'>ab7909</a>) at 10 µg

Lanes 4 and 8:

Western blot - HEK-293 whole cell lysate (<a href='/en-us/products/cell-lysates/hek-293-whole-cell-lysate-ab7902'>ab7902</a>) at 10 µg

Predicted band size: 36 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p>Milk blocking may cause 'no bands' problem. Please try BSA as well.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Saccharomyces cerevisiae": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.

Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed : 11724794, PubMed : 3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed : 11724794, PubMed : 3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed : 23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed : 23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed : 23332158, PubMed : 27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).

See full target information GAPDH

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Publications (106)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:21130 PubMed39256509

2024

Morphine promotes non-small cell lung cancer progression by downregulating E-cadherin via the PI3K/AKT/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fulei Gu,Yuxuan Zhou,Lili Tian,Jinyan Chen,Can Zhang,Zhangxiang Huang,Weifeng Yu,Kangjie Xie

Glia 72:1915-1929 PubMed38982826

2024

Connexin 30 locally controls actin cytoskeleton and mechanical remodeling in motile astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Grégory Ghézali,Jérôme Ribot,Nathan Curry,Laure-Elise Pillet,Flora Boutet-Porretta,Daria Mozheiko,Charles-Félix Calvo,Pascal Ezan,Isabelle Perfettini,Laure Lecoin,Sébastien Janel,Jonathan Zapata,Carole Escartin,Sandrine Etienne-Manneville,Clemens F Kaminski,Nathalie Rouach

Frontiers in oncology 13:1181176 PubMed37916167

2023

immunotherapy combined with gemcitabine has a synergistic inhibitory effect on tumor growth and metastasis in murine Lewis lung cancer models.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Chen,Zhu Tao,Yun Liang,Meng Ma,Dickson Adah,Wenting Ding,Lili Chen,Xiaofen Li,Linglin Dai,Songwe Fanuel,Siting Zhao,Wen Hu,Donghai Wu,Ziyuan Duan,Fang Zhou,Li Qin,Xiaoping Chen,Zhaoqing Yang

Nature communications 14:2057 PubMed37045813

2023

Targeting neuronal lysosomal dysfunction caused by β-glucocerebrosidase deficiency with an enzyme-based brain shuttle construct.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Gehrlein,Vinod Udayar,Nadia Anastasi,Martino L Morella,Iris Ruf,Doris Brugger,Sophia von der Mark,Ralf Thoma,Arne Rufer,Dominik Heer,Nina Pfahler,Anton Jochner,Jens Niewoehner,Luise Wolf,Matthias Fueth,Martin Ebeling,Roberto Villaseñor,Yanping Zhu,Matthew C Deen,Xiaoyang Shan,Zahra Ehsaei,Verdon Taylor,Ellen Sidransky,David J Vocadlo,Per-Ola Freskgård,Ravi Jagasia

Journal of cell science 136: PubMed36695453

2023

Lamin A/C phosphorylation at serine 22 is a conserved heat shock response to regulate nuclear adaptation during stress.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Virtanen,Emilia Holm,Mona Halme,Gun West,Fanny Lindholm,Josef Gullmets,Juho Irjala,Tiina Heliö,Artur Padzik,Annika Meinander,John E Eriksson,Pekka Taimen

Cancers 15: PubMed36765842

2023

Cerivastatin Synergizes with Trametinib and Enhances Its Efficacy in the Therapy of Uveal Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana Agnese Amaro,Rosaria Gangemi,Laura Emionite,Patrizio Castagnola,Gilberto Filaci,Martine J Jager,Enrica Teresa Tanda,Francesco Spagnolo,Matteo Mascherini,Ulrich Pfeffer,Michela Croce

Journal of cell science 135: PubMed36093837

2022

Distinct roles of the Chlamydia trachomatis effectors TarP and TmeA in the regulation of formin and Arp2/3 during entry.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew D Romero,Rey A Carabeo

Molecular therapy. Nucleic acids 29:923-940 PubMed36032397

2022

Self-assembling short immunostimulatory duplex RNAs with broad-spectrum antiviral activity.

Applications

Unspecified application

Species

Unspecified reactive species

Longlong Si,Haiqing Bai,Crystal Yuri Oh,Amanda Jiang,Fan Hong,Tian Zhang,Yongxin Ye,Tristan X Jordan,James Logue,Marisa McGrath,Chaitra Belgur,Karina Calderon,Atiq Nurani,Wuji Cao,Kenneth E Carlson,Rachelle Prantil-Baun,Steven P Gygi,Dong Yang,Colleen B Jonsson,Benjamin R tenOever,Matthew Frieman,Donald E Ingber

Molecular medicine (Cambridge, Mass.) 28:5 PubMed35062861

2022

Pro-inflammatory mediators released by activated monocytes promote aortic valve fibrocalcific activity.

Applications

Unspecified application

Species

Unspecified reactive species

Peijian Zhang,Erlinda The,Zichao Luo,Yufeng Zhai,Qingzhou Yao,Lihua Ao,David A Fullerton,Dingli Xu,Xianzhong Meng

Molecular medicine reports 25: PubMed35039872

2022

Long non‑coding RNA CTBP1‑AS2 upregulates USP22 to promote pancreatic carcinoma progression by sponging miR‑141‑3p.

Applications

Unspecified application

Species

Unspecified reactive species

Mingliang Zhang,Songbo Ma,Xuzhao Li,Henghai Yu,Yizheng Tan,Jun He,Xiaoping Wei,Junming Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-GAPDH antibody - Loading Control

Western blot - HRP Anti-GAPDH antibody - Loading Control (AB9385)

Western blot - HRP Anti-GAPDH antibody - Loading Control (AB9385)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (106)

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