Anti-GAPDH [mAbcam 9484] - Loading Control HRP conjugated antibody (ab9482) mouse monoclonal antibody that is used to detect GAPDH in Western Blot. Suitable for Human, Mouse, Rat samples.
- Over 220 publications
- Trusted since 2004
Images
![Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (AB9482), expandable thumbnail](https://content.abcam.com/products/images/hrp-gapdh-antibody-mabcam-9484-loading-control-ab9482--western-blot-img95251.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (AB9482)")
Publications
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- Cellular and molecular life sciences : CMLS 80:3782023Mistranslation-associated perturbations of proteostasis do not promote accumulation of amyloid beta and plaque deposition in aged mouse brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHarshitha Santhosh Kumar,James Moore,Adrian C Steiner,Emmanuel Sotirakis,Benjamin Schärli,Patricia Isnard-Petit,Kader Thiam,David P Wolfer,Erik C BöttgerPubMed 38010524
- Genome biology 24:2162023A loss-of-function mutation in human Oxidation Resistance 1 disrupts the spatial-temporal regulation of histone arginine methylation in neurodevelopment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolin Lin,Wei Wang,Mingyi Yang,Nadirah Damseh,Mirta Mittelstedt Leal de Sousa,Fadi Jacob,Anna Lång,Elise Kristiansen,Marco Pannone,Miroslava Kissova,Runar Almaas,Anna Kuśnierczyk,Richard Siller,Maher Shahrour,Motee Al-Ashhab,Bassam Abu-Libdeh,Wannan Tang,Geir Slupphaug,Orly Elpeleg,Stig Ove Bøe,Lars Eide,Gareth J Sullivan,Johanne Egge Rinholm,Hongjun Song,Guo-Li Ming,Barbara van Loon,Simon Edvardson,Jing Ye,Magnar BjøråsPubMed 37773136
- eLife 12:2023Mitochondrial protein import clogging as a mechanism of disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiam P Coyne,Xiaowen Wang,Jiyao Song,Ebbing de Jong,Karin Schneider,Paul T Massa,Frank A Middleton,Thomas Becker,Xin Jie ChenPubMed 37129366
- iScience 26:1065292023Design of diversified chimeric antigen receptors through rational module recombination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Si,Ying-Ying Fan,Shi-Zhen Qiu,Xin Li,Er-Yi Wu,Jian-Qi Ju,Wen Huang,Hao-Peng Wang,Ping WeiPubMed 37102149
- International journal of molecular sciences 24:2023MicroRNA-200c-5p Regulates Migration and Differentiation of Myoblasts via Targeting in Skeletal Muscle Regeneration and Myogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanwen Liu,Yilong Yao,Yongsheng Zhang,Chao Yan,Mingsha Yang,Zishuai Wang,Wangzhang Li,Fanqinyu Li,Wei Wang,Yalan Yang,Xinyun Li,Zhonglin TangPubMed 36902425
- International journal of molecular sciences 24:2023Proximity Labeling to Identify β-Arrestin1 Binding Partners Downstream of Ligand-Activated G Protein-Coupled Receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYa Zhuo,Valeria L Robleto,Adriano MarchesePubMed 36834700
- iScience 26:1058812023loss in pancreas leads to islet developmental defect and metabolic disturbance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTzu-Lei Kuo,Kuang-Hung Cheng,Li-Tzong Chen,Wen-Chun HungPubMed 36654862
- Molecular brain 16:92023Cell-type-specific translational control of spatial working memory by the cap-binding protein 4EHP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShane Wiebe,Ziying Huang,Reese Jalal Ladak,Agnieszka Skalecka,Roberta Cagnetta,Jean-Claude Lacaille,Argel Aguilar-Valles,Nahum SonenbergPubMed 36650535
- Open medicine (Warsaw, Poland) 17:1883-18952022TET3 governs malignant behaviors and unfavorable prognosis of esophageal squamous cell carcinoma by activating the PI3K/AKT/GSK3β/β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaoling Zhu,Bowen Shi,Chunguang Li,Shuchang XuPubMed 36518116
- Frontiers in genetics 13:9899122022miR-1306 induces cell apoptosis by targeting BMPR1B gene in the ovine granulosa cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnwar Abdurahman,Wusimanjiang Aierken,Fei Zhang,Rahmantay Obulkasim,Jueken Aniwashi,Ablat SulaymanPubMed 36212145
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Conjugation
- HRP
- Storage buffer
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| Chicken | Predicted |
| Chinese hamster | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Rabbit | Predicted |
| Xenopus laevis | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484). This change in characteristics of the ab is due to a recent update to the production process. |
Species Rat | Dilution info 1/1000 - 1/5000 | Notes NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484). This change in characteristics of the ab is due to a recent update to the production process. |
Species Human | Dilution info 1/1000 - 1/5000 | Notes NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484). This change in characteristics of the ab is due to a recent update to the production process. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Chicken, Cow, Dog, Xenopus laevis, Chinese hamster | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Version
5 products for Alternative Product
Target data
Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:11724794, PubMed:3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:11724794, PubMed:3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
Alternative names
GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH
Recommended products
Anti-GAPDH [mAbcam 9484] - Loading Control HRP conjugated antibody (ab9482) mouse monoclonal antibody that is used to detect GAPDH in Western Blot. Suitable for Human, Mouse, Rat samples.
- Over 220 publications
- Trusted since 2004
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Conjugation
- HRP
- Storage buffer
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- mAbcam 9484
- Purity
- IgG fraction
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.
What is the molecular weight of GAPDH?
Anti-GAPDH [mAbcam 9484] - Loading Control HRP (ab9482) is often used as a loading control in Western blot. It specifically detects a band for GAPDH (UniProt: P04406) at a molecular weight of 40.2kDa.
Trusted by the scientific community
Anti-GAPDH [mAbcam 9484] - Loading Control HRP (ab9482) was first used in a scientific publication in 2004 and has been cited over 220 times in peer-reviewed journals.
Reviewed by scientists
Anti-GAPDH [mAbcam 9484] - Loading Control HRP (ab9482) has over 15 independent reviews from customers.
Related products
Antibody clone mAbcam 9484 is also available pre-conjugated to a variety of labels for your convenience – Anti-GAPDH HRP [mAbcam 9484] - Loading Control (ab9482).
Other related products
We have a range of other formats of antibody clone [mAbcam 9484] also available for your convenience:
Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, Alexa Fluor® 680 - Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab184095, Alexa Fluor® 790 - Alexa Fluor® 790 Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab184578, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-GAPDH antibody [mAbcam 9484] ab196697
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
- Biological function summary
GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.
- Pathways
GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.
- Associated diseases and disorders
GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482), expandable thumbnail](https://content.abcam.com/products/images/hrp-gapdh-antibody-mabcam-9484-loading-control-ab9482--western-blot-img95251.jpg "Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)")
Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)
All lanes: Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482) at 1/1000 dilution
Lane 1: HeLa Whole Cell Lysate at 10 µg
Lane 2: Hek293 Whole Cell Lysate at 10 µg
Lane 3: NIH 3T3 Whole Cel lLysate at 10 µg
Lane 4: PC12 Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 37 kDa
Exposure time: 4min
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Product protocols
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