AB6663

HRP Anti-GFP antibody

Name: HRP conjugated Anti-GFP antibody (ab6663) | Abcam
Brand: Abcam Limited
SKU: AB6663
Price: 535 USD
Availability: InStock

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(45 Publications)

Anti-GFP antibody - HRP conjugated (ab6663) is a goat polyclonal antibody detecting GFP in Western Blot.

- Over 40 publications
- Trusted since 2002

See more GFP antibodies and assay kits

View Alternative Names

Green fluorescent protein, GFP

1 Images

Supplier Data

Western blot - HRP Anti-GFP antibody (AB6663)

All lanes:

Western blot - HRP Anti-GFP antibody (ab6663)

All lanes:

GFP

Predicted band size: 27 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Aequorea victoria GFP.

P42212

Specificity

No reaction was observed against Human, Mouse and Rat Serum Proteins.

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Reactivity data

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Product details

What is this antibody validated in?
HRP Anti-GFP antibody (ab6663) is a goat polyclonal antibody and is validated for use in Western Blot (WB). Trusted by the scientific community
Anti-GFP HRP (ab6663) was first used in a scientific publication in 2002 and has been cited over 40 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

See full target information GFP

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Publications (45)

Recent publications for all applications. Explore the full list and refine your search

BMC biology 21:73 PubMed37024974

2023

The UAS thioredoxin-like domain of UBXN7 regulates E3 ubiquitin ligase activity of RNF111/Arkadia.

Applications

Unspecified application

Species

Unspecified reactive species

Sadek Amhaz,Batiste Boëda,Mouna Chouchène,Sabrina Colasse,Florent Dingli,Damarys Loew,Julien Henri,Céline Prunier,Laurence Levy

The EMBO journal 42:e111484 PubMed36592032

2023

Effector-dependent activation and oligomerization of plant NRC class helper NLRs by sensor NLR immune receptors Rpi-amr3 and Rpi-amr1.

Applications

Unspecified application

Species

Unspecified reactive species

Hee-Kyung Ahn,Xiao Lin,Andrea Carolina Olave-Achury,Lida Derevnina,Mauricio P Contreras,Jiorgos Kourelis,Chih-Hang Wu,Sophien Kamoun,Jonathan D G Jones

The New phytologist 237:1270-1284 PubMed36333900

2022

Phosphorylation status of CPK28 affects its ubiquitination and protein stability.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaotong Liu,Yuanyuan Zhou,Kexin Chen,Zejun Xiao,Xuelian Liang,Dongping Lu

Biotechnology journal 17:e2100504 PubMed35332696

2022

Simplifying plant gene silencing and genome editing logistics by a one-Agrobacterium system for simultaneous delivery of multipartite virus vectors.

Applications

Unspecified application

Species

Unspecified reactive species

Verónica Aragonés,Flavio Aliaga,Fabio Pasin,José-Antonio Daròs

American journal of respiratory cell and molecular biology 66:402-414 PubMed35045271

2022

Neonatal Hyperoxia Activates Activating Transcription Factor 4 to Stimulate Folate Metabolism and Alveolar Epithelial Type 2 Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Min Yee,Andrew N McDavid,Ethan David Cohen,Heidie L Huyck,Cory Poole,Brian J Altman,William M Maniscalco,Gail H Deutsch,Gloria S Pryhuber,Michael A O'Reilly

Scientific reports 11:23977 PubMed34907219

2021

Differentiation of cancer stem cells into erythroblasts in the presence of CoCl.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuki Kumon,Said M Afify,Ghmkin Hassan,Shunsuke Ueno,Sadia Monzur,Hend M Nawara,Hagar A Abu Quora,Mona Sheta,Yanning Xu,Xiaoying Fu,Maram H Zahra,Akimasa Seno,Masaharu Seno

PloS one 16:e0257089 PubMed34793485

2021

Recombinant production of a functional SARS-CoV-2 spike receptor binding domain in the green algae Chlamydomonas reinhardtii.

Applications

Unspecified application

Species

Unspecified reactive species

Anthony J Berndt,Tressa N Smalley,Bijie Ren,Ryan Simkovsky,Amr Badary,Ashley E Sproles,Francis J Fields,Yasin Torres-Tiji,Vanessa Heredia,Stephen P Mayfield

Nature communications 12:4327 PubMed34267202

2021

Lanthanum(III) triggers AtrbohD- and jasmonic acid-dependent systemic endocytosis in plants.

Applications

Unspecified application

Species

Unspecified reactive species

Mengzhu Cheng,Lihong Wang,Qing Zhou,Daiyin Chao,Shingo Nagawa,Ding He,Jiazhi Zhang,Hui Li,Li Tan,Zhenhong Gu,Xiaohua Huang,Zhenbiao Yang

Frontiers in plant science 12:667242 PubMed34040624

2021

Overexpression of Accelerates the Transition to Flowering and Increases Plant Height in Soybean.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Yang,Xin Li,Jinming Shan,Yinghua Li,Yuntong Zhang,Yuhe Wang,Wenbin Li,Lin Zhao

Scientific reports 10:5001 PubMed32193417

2020

Nuclear receptor CAR-ERα signaling regulates the estrogen sulfotransferase gene in the liver.

Applications

Unspecified application

Species

Unspecified reactive species

MyeongJin Yi,Muluneh Fashe,Shingo Arakawa,Rick Moore,Tatsuya Sueyoshi,Masahiko Negishi

View all publications

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