JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB6663

HRP Anti-GFP antibody

Be the first to review this product! Submit a review

|

(59 Publications )

Anti-GFP antibody conjugated to HRP is a goat polyclonal antibody suitable for Western blot.
1 Images
Western blot - HRP Anti-GFP antibody (AB6663)
  • WB

Supplier Data

Western blot - HRP Anti-GFP antibody (AB6663)

Marker : Opal Pre-stained MW ladder.

Blocking Buffer : Universal Blocking Buffer(Rockland) for 30 min at RT.

All lanes:

Western blot - HRP Anti-GFP antibody (ab6663) at 1/1000 dilution

Lane 2:

HEK293 (human embryonic kidney cells) cell lysate at 10 µg

Lane 3:

HeLa cell Lysate at 10 µg

Lane 4:

CHO/K1 (Chinese Hamster Ovary cell line) cell Lysate at 10 µg

Lane 5:

C2C12 (mouse myoblast cell line) cell Lysate at 10 µg

Lane 6:

NIH/3T3 (mouse embryonic fibroblast cell line) cell Lysate at 10 µg

Lane 7:

MEFs (mouse embryonic fibroblast cells) cell Lysate at 10 µg

Lane 8:

E-coli Host Cell Proteins (HCP) Control (purified reference proteins) at 50 µg

Lane 9:

2 Epitope MBP Tag Marker Lysate at 10 µg

Lane 10:

Recombinant Red Fluorescent Protein at 50 µg

Lane 11:

Green Fluorescent Protein at 50 µg

Lane 12:

Glutathione-STransferase Protein at 50 µg

Lane 13:

Maltose Binding Protein at 50 µg

Observed band size: 28 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Aequorea victoria GFP.

P42212

Specificity

<p>No reaction was observed against Human, Mouse and Rat Serum Proteins.</p>

style
left-column

Alternative Products

Primary Antibodies

AB190584

HRP Anti-GFP antibody [E385]

RabMAb|Recombinant

Western blot - HRP Anti-GFP antibody [E385] (AB190584)

  • 5
  • 1
Primary Antibodies

AB236117

Anti-GFP antibody [EPR14104-89] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (AB236117)

  • 4
  • 1
Primary Antibodies

AB183735

Anti-GFP antibody [EPR14104-89]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104-89] (AB183735)

  • 0
  • 0
Primary Antibodies

AB220802

Anti-GFP antibody [EPR14104] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104] - BSA and Azide free (AB220802)

  • 4
  • 1
Primary Antibodies

AB239807

Anti-GFP antibody [E385] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-GFP antibody [E385] - BSA and Azide free (AB239807)

  • 5
  • 1
Primary Antibodies

AB32146

Anti-GFP antibody [E385]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-GFP antibody [E385] (AB32146)

  • 5
  • 4

Complementary Products

Primary Antibodies

AB167453

Anti-mCherry antibody

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (AB167453)

  • 5
  • 17
Primary Antibodies

AB34771

Biotin Anti-RFP antibody

Western blot - Biotin Anti-RFP antibody (AB34771)

  • 4
  • 5
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

What is this antibody validated in?
HRP Anti-GFP antibody (ab6663) is a goat polyclonal antibody and is validated for use in Western Blot (WB). Trusted by the scientific community
Anti-GFP HRP (ab6663) was first used in a scientific publication in 2002 and has been cited over 40 times in peer-reviewed journals.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP

Alternative Names

Green fluorescent protein, GFP
style
left-column

Publications (59)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in plant science 15:1425700 PubMed40528925

2025

Overexpression of TaPIP1A enhances drought and salt stress tolerance in Arabidopsis: cross-species conservation and molecular dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Han,Wei Zhang,Liya Zhi,Silong Chen,Hui Zhao,Xinyu Zhang,Liuge Xue,Jun Ji,Fengzhi Wang,Junming Li

Science advances 11:eadr9340 PubMed39937906

2025

VARP binds SNX27 to promote endosomal supercomplex formation on membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Mintu Chandra,Amy K Kendall,Marijn G J Ford,Lauren P Jackson

FEBS letters 599:1029-1045 PubMed39912397

2025

Integrated interactome, proteomic and functional analyses reveal molecular pathways driving L1TD1-induced aggressiveness in CNS embryonal tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Thiago Giove Mitsugi,Matt Sherwood,Elisa Helena Farias Jandrey,Amanda Faria Assoni,Joseph Bell,Brandon Coke,Paul Skipp,Rob Ewing,Oswaldo Keith Okamoto

Molecules and cells 47:100158 PubMed39577746

2024

An RXLR effector disrupts vesicle trafficking at ER-Golgi interface for Phytophthora capsici pathogenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Jihyun Kim,Jesse Kaleku,Haeun Kim,Minji Kang,Hui Jeong Kang,Jongchan Woo,Hongshi Jin,Seungmee Jung,Cécile Segonzac,Eunsook Park,Doil Choi

Journal of integrative plant biology 66:1603-1619 PubMed38869305

2024

The MADS-box transcription factor GmFULc promotes GmZTL4 gene transcription to modulate maturity in soybean.

Applications

Unspecified application

Species

Unspecified reactive species

Jingzhe Sun,Yucheng Liu,Yuhong Zheng,Yongguo Xue,Yuhuan Fan,Xiaofei Ma,Yujia Ji,Gaoyuan Liu,Xiaoming Zhang,Yang Li,Shuming Wang,Zhixi Tian,Lin Zhao

eLife 12: PubMed38727576

2024

Coupling and uncoupling of midline morphogenesis and cell flow in amniote gastrulation.

Applications

Unspecified application

Species

Unspecified reactive species

Rieko Asai,Vivek N Prakash,Shubham Sinha,Manu Prakash,Takashi Mikawa

Scientific reports 14:10091 PubMed38698065

2024

Discovery of active mouse, plant and fungal cytochrome P450s in endogenous proteomes and upon expression in planta.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Font-Farre,Daniel Brown,Reka Toth,Chidambareswaren Mahadevan,Melissa Brazier-Hicks,Kyoko Morimoto,Farnusch Kaschani,John Sinclair,Richard Dale,Samantha Hall,Melloney Morris,Markus Kaiser,Aaron T Wright,Jonathan Burton,Renier A L van der Hoorn

mSystems 9:e0022124 PubMed38546227

2024

remains translationally active after CRISPRi-mediated replication initiation arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Muñoz-Gutierrez,Fabián A Cornejo,Katja Schmidt,Christian K Frese,Manuel Halte,Marc Erhardt,Alexander K W Elsholz,Kürşad Turgay,Emmanuelle Charpentier

The Journal of cell biology 223: PubMed38386112

2024

The distinct localization of CDC42 isoforms is responsible for their specific functions during migration.

Applications

Unspecified application

Species

Unspecified reactive species

Yamini Ravichandran,Jan Hänisch,Kerren Murray,Vanessa Roca,Florent Dingli,Damarys Loew,Valentin Sabatet,Batiste Boëda,Theresia E Stradal,Sandrine Etienne-Manneville

Molecular biology of the cell 35:ar56 PubMed38381558

2024

Tepsin binds LC3B to promote ATG9A trafficking and delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie S Wallace,John E Gadbery,Cameron I Cohen,Amy K Kendall,Lauren P Jackson
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com