AB190584

HRP Anti-GFP antibody [E385]

Name: Anti-GFP HRP antibody [E385] (ab190584) Rabbit monoclonal IgG | Abcam
Brand: Abcam Limited
SKU: AB190584
Price: 620 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(16 Publications)

Rabbit Recombinant Monoclonal GFP antibody - conjugated to HRP. Suitable for WB. Cited in 9 publications.

See more GFP antibodies and assay kits

View Alternative Names

Green fluorescent protein, GFP

2 Images

Lab

Western blot - HRP Anti-GFP antibody [E385] (AB190584)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190485 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-GFP antibody [E385] (ab190584) at 1/10000 dilution

Lane 1:

HEK 293 over-expressing GFP at 5 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 5 µg

Predicted band size: 27 kDa

Observed band size: 27 kDa

true

Exposure time: 30s

Lab

Western blot - HRP Anti-GFP antibody [E385] (AB190584)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190584 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-GFP antibody [E385] (ab190584) at 1/10000 dilution

Lane 1:

Western blot - Recombinant A. victoria GFP protein (<a href='/en-us/products/proteins-peptides/recombinant-a-victoria-gfp-protein-ab84191'>ab84191</a>) at 0.1 µg

Lane 2:

Western blot - Recombinant RFP protein (<a href='/en-us/products/proteins-peptides/recombinant-rfp-protein-ab51993'>ab51993</a>) at 0.1 µg

Predicted band size: 27 kDa

Observed band size: 27 kDa

true

Exposure time: 10s

Unconjugated

Anti-GFP antibody [E385]
Carrier free

Anti-GFP antibody [E385] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E385

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2026, QC testing of replenishment batches of this primary conjugate changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, the replenishment lots are subjected to bioanalytical testing only. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

See full target information GFP

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Journal of lipid research 65:100663 PubMed39369791

2024

A fatty acid-ordered plasma membrane environment is critical for Ebola virus matrix protein assembly and budding.

Applications

Unspecified application

Species

Unspecified reactive species

Souad Amiar,Kristen A Johnson,Monica L Husby,Andrea Marzi,Robert V Stahelin

Nature communications 15:4322 PubMed38773107

2024

The SUN-family protein Sad1 mediates heterochromatin spatial organization through interaction with histone H2A-H2B.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqi Sun,Qianhua Dong,Xueqing Li,Jinxin Gao,Xianwen Ye,Chunyi Hu,Fei Li,Yong Chen

Nature communications 15:2399 PubMed38493158

2024

The spliceosome-associated protein CWC15 promotes miRNA biogenesis in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Bangjun Zhou,Huihui Yu,Yong Xue,Mu Li,Chi Zhang,Bin Yu

Science advances 9:eadi9036 PubMed37967186

2023

ACD15, ACD21, and SLN regulate the accumulation and mobility of MBD6 to silence genes and transposable elements.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon A Boone,Lucia Ichino,Shuya Wang,Jason Gardiner,Jaewon Yun,Yasaman Jami-Alahmadi,Jihui Sha,Cristy P Mendoza,Bailey J Steelman,Aliya van Aardenne,Sophia Kira-Lucas,Isabelle Trentchev,James A Wohlschlegel,Steven E Jacobsen

The New phytologist 240:1467-1483 PubMed37658678

2023

Plant immunity suppressor SKRP encodes a novel RNA-binding protein that targets exon 3' end of unspliced RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Chen,Zhihui Xu,Jie Huang,Haidong Shu,Yufan Hui,Danling Zhu,Yufeng Wu,Suomeng Dong,Zhe Wu

ACS chemical biology 18:583-594 PubMed36795767

2023

Screen for Modulation of Nucleocapsid Protein Condensation Identifies Small Molecules with Anti-Coronavirus Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Tong Quek,Kierra S Hardy,Stephen G Walker,Dan T Nguyen,Taciani de Almeida Magalhães,Adrian Salic,Sujatha M Gopalakrishnan,Pamela A Silver,Timothy J Mitchison

Biochemical and biophysical research communications 639:54-61 PubMed36470072

2022

Ser815 Phosphorylation stabilizes the androgen receptor homodimer and stimulates ER-stress induced cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Kosuke Yokobori,Masahiko Negishi

Plant biotechnology journal 20:1363-1372 PubMed35325498

2022

Plant-based expression and characterization of SARS-CoV-2 virus-like particles presenting a native spike protein.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Wan Jung,Gergana Zahmanova,Ivan Minkov,George P Lomonossoff

eLife 10: PubMed34397383

2021

A CTP-dependent gating mechanism enables ParB spreading on DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Sb Jalal,Ngat T Tran,Clare Em Stevenson,Afroze Chimthanawala,Anjana Badrinarayanan,David M Lawson,Tung Bk Le

Science advances 7: PubMed33523899

2021

Overlap of NatA and IAP substrates implicates N-terminal acetylation in protein stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Franziska Mueller,Alexandra Friese,Claudio Pathe,Richard Cardoso da Silva,Kenny Bravo Rodriguez,Andrea Musacchio,Tanja Bange

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-GFP antibody [E385]

Western blot - HRP Anti-GFP antibody [E385] (AB190584)

Western blot - HRP Anti-GFP antibody [E385] (AB190584)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (16)

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