AB137840

HRP Anti-GST antibody

Name: Anti-GST HRP antibody (ab137840) Rabbit polyclonal IgG | Abcam
Brand: Abcam Limited
SKU: AB137840

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(1 Publication)

Rabbit Polyclonal GST antibody - conjugated to HRP. Suitable for WB and reacts with Tag samples. Cited in 1 publication.

View Alternative Names

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

1 Images

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Western blot - HRP Anti-GST antibody (AB137840)

12% SDS PAGE

All lanes:

Western blot - HRP Anti-GST antibody (ab137840) at 1/1000 dilution

Lane 1:

BSA 20 ng

Lane 2:

GST 20 ng

Lane 3:

GST 10 ng

Lane 4:

GST 1 ng

Predicted band size: 25 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Applications

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab137840 was immunoaffinity purified using GST immobilized on a solid support and conjugated with HRP

Storage buffer

pH: 7.4 Preservative: 0.005% Thimerosal (merthiolate) Constituents: 98% Tris buffered saline, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.

Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

See full target information Glutathione S-transferase class-mu 26 kDa isozyme

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 291:24096-24104 PubMed27650500

2016

A Histidine Cluster in the Cytoplasmic Domain of the Na-H Exchanger NHE1 Confers pH-sensitive Phospholipid Binding and Regulates Transporter Activity.

Applications

Species

Unspecified reactive species

Bradley A Webb,Katharine A White,Bree K Grillo-Hill,André Schönichen,Changhoon Choi,Diane L Barber

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