AB3416

HRP Anti-GST antibody

Name: HRP Anti-GST antibody (ab3416) | Abcam
Brand: Abcam Limited
SKU: AB3416
Rating: 5 (3 reviews)

(3 Reviews)

(38 Publications)

Anti-GST antibody - HRP conjugated (ab3416) is a rabbit polyclonal antibody detecting GST in Western Blot, ELISA.

- Over 30 publications
- Trusted since 2003

View Alternative Names

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

2 Images

ELISA

AbReview46320****

ELISA - HRP Anti-GST antibody (AB3416)

Graph of ELISA measuring levels of bound GST-tagged mosquito proteins. 5g of target protein was coated onto an ELISA plate and incubated overnight at 4°C. The plate was washed and GST-tagged mosquito proteins were incubated for an hour at RT. The proteins were washed off, GST-HRP antibody (ab3416) was diluted 1/5000 in 1%BSA in PBS and added for 30 min at RT. The plate was washed and TMB substrate was added for 20 min at RT. Stop solution was added and the O.D. of the wells read at 450 nm.

This image is courtesy of an anonymous abreview.

ELISA

AbReview46487****

ELISA - HRP Anti-GST antibody (AB3416)

This image is courtesy of an anonymous abreview.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Applications

ELISA, WB

applications

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Reactivity data

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Product details

What is this antibody validated in?
HRP Anti-GST antibody (ab3416) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), ELISA. Trusted by the scientific community
Anti-GST HRP (ab3416) was first used in a scientific publication in 2003 and has been cited over 30 times in peer-reviewed journals.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Immunoaffinity purified using GST immobilized on a solid support.

Storage buffer

pH: 7 Constituents: 15% Sodium chloride, 3% Triton X-100, 3% Sodium deoxycholate, 0.3% Tris HCl

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.

Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

See full target information Glutathione S-transferase class-mu 26 kDa isozyme

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Publications (38)

Recent publications for all applications. Explore the full list and refine your search

Genome biology 26:155 PubMed40468392

2025

DNA G-quadruplex structures act as functional elements in α- and β-globin enhancers.

Applications

Unspecified application

Species

Unspecified reactive species

Colm Doyle,Krzysztof Herka,Sean M Flynn,Larry Melidis,Somdutta Dhir,Stefan Schoenfelder,David Tannahill,Shankar Balasubramanian

PloS one 20:e0318295 PubMed39903729

2025

A universal method for the purification of C2H2 zinc finger arrays.

Applications

Unspecified application

Species

Unspecified reactive species

Jingchang Liang,Maia Azubel,Guanqiao Wang,Yan Nie,Roger D Kornberg,Andrew J Beel,Pierre-Jean Mattei

Proceedings of the National Academy of Sciences of the United States of America 120:e2310126120 PubMed37934824

2023

FAB1C, a phosphatidylinositol 3-phosphate 5-kinase, interacts with PIN-FORMEDs and modulates their lytic trafficking in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Kwang-Ho Maeng,Hyodong Lee,Hyung-Taeg Cho

Molecular cell 83:1743-1760.e11 PubMed37116497

2023

Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Edoardo José Longarini,Helen Dauben,Carolina Locatelli,Anne R Wondisford,Rebecca Smith,Charlotte Muench,Andreas Kolvenbach,Michelle Lee Lynskey,Alexis Pope,Juan José Bonfiglio,Eva Pinto Jurado,Roberta Fajka-Boja,Thomas Colby,Marion Schuller,Ivan Ahel,Gyula Timinszky,Roderick J O'Sullivan,Sébastien Huet,Ivan Matic

microPublication biology 2023: PubMed37038481

2023

SAF-A/hnRNP U binds polyphosphoinositides via a lysine rich polybasic motif located in the SAP domain.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda J Edson,Rhîan G Jacobsen,Aurélia E Lewis

Life science alliance 6: PubMed36882285

2023

The TUDOR domain of SMN is an H3K79 histone mark reader.

Applications

Unspecified application

Species

Unspecified reactive species

Olivier Binda,Aimé Boris Kimenyi Ishimwe,Maxime Galloy,Karine Jacquet,Armelle Corpet,Amélie Fradet-Turcotte,Jocelyn Côté,Patrick Lomonte

Life science alliance 6: PubMed36375840

2022

SMA-linked SMN mutants prevent phase separation properties and SMN interactions with FMRP family members.

Applications

Unspecified application

Species

Unspecified reactive species

Olivier Binda,Franceline Juillard,Julia Novion Ducassou,Constance Kleijwegt,Geneviève Paris,Andréanne Didillon,Faouzi Baklouti,Armelle Corpet,Yohann Couté,Jocelyn Côté,Patrick Lomonte

Cell death & disease 13:575 PubMed35773260

2022

p21-activated kinase 4 controls the aggregation of α-synuclein by reducing the monomeric and aggregated forms of α-synuclein: involvement of the E3 ubiquitin ligase NEDD4-1.

Applications

Unspecified application

Species

Unspecified reactive species

So-Yoon Won,Jung-Jin Park,Soon-Tae You,Jong-A Hyeun,Hyong-Kyu Kim,Byung Kwan Jin,Catriona McLean,Eun-Young Shin,Eung-Gook Kim

Proceedings of the National Academy of Sciences of the United States of America 119: PubMed35115407

2022

TBP-ASSOCIATED FACTOR 12 ortholog NOBIRO6 controls root elongation with unfolded protein response cofactor activity.

Applications

Unspecified application

Species

Unspecified reactive species

June-Sik Kim,Yuki Sakamoto,Fuminori Takahashi,Michitaro Shibata,Kaoru Urano,Sachihiro Matsunaga,Kazuko Yamaguchi-Shinozaki,Kazuo Shinozaki

The Plant cell 33:3513-3531 PubMed34402905

2021

Calcium-dependent protein kinase 29 modulates PIN-FORMED polarity and Arabidopsis development via its own phosphorylation code.

Applications

Unspecified application

Species

Unspecified reactive species

Hyodong Lee,Anindya Ganguly,Song Baik,Hyung-Taeg Cho

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-GST antibody

ELISA - HRP Anti-GST antibody (AB3416)

ELISA - HRP Anti-GST antibody (AB3416)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (38)

Product promise