Anti-GST antibody - HRP conjugated (ab3416) is a rabbit polyclonal antibody detecting GST in Western Blot, ELISA.
- Over 30 publications
- Trusted since 2003
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- Molecular cell 83:1743-1760.e112023Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdoardo José Longarini,Helen Dauben,Carolina Locatelli,Anne R Wondisford,Rebecca Smith,Charlotte Muench,Andreas Kolvenbach,Michelle Lee Lynskey,Alexis Pope,Juan José Bonfiglio,Eva Pinto Jurado,Roberta Fajka-Boja,Thomas Colby,Marion Schuller,Ivan Ahel,Gyula Timinszky,Roderick J O'Sullivan,Sébastien Huet,Ivan MaticPubMed 37116497
- Life science alliance 6:2023The TUDOR domain of SMN is an H3K79 histone mark reader.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlivier Binda,Aimé Boris Kimenyi Ishimwe,Maxime Galloy,Karine Jacquet,Armelle Corpet,Amélie Fradet-Turcotte,Jocelyn Côté,Patrick LomontePubMed 36882285
- Life science alliance 6:2022SMA-linked SMN mutants prevent phase separation properties and SMN interactions with FMRP family members.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlivier Binda,Franceline Juillard,Julia Novion Ducassou,Constance Kleijwegt,Geneviève Paris,Andréanne Didillon,Faouzi Baklouti,Armelle Corpet,Yohann Couté,Jocelyn Côté,Patrick LomontePubMed 36375840
- Cell death & disease 13:5752022p21-activated kinase 4 controls the aggregation of α-synuclein by reducing the monomeric and aggregated forms of α-synuclein: involvement of the E3 ubiquitin ligase NEDD4-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSo-Yoon Won,Jung-Jin Park,Soon-Tae You,Jong-A Hyeun,Hyong-Kyu Kim,Byung Kwan Jin,Catriona McLean,Eun-Young Shin,Eung-Gook KimPubMed 35773260
- Proceedings of the National Academy of Sciences of the United States of America 119:2022TBP-ASSOCIATED FACTOR 12 ortholog NOBIRO6 controls root elongation with unfolded protein response cofactor activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJune-Sik Kim,Yuki Sakamoto,Fuminori Takahashi,Michitaro Shibata,Kaoru Urano,Sachihiro Matsunaga,Kazuko Yamaguchi-Shinozaki,Kazuo ShinozakiPubMed 35115407
- The Plant cell 33:3513-35312021Calcium-dependent protein kinase 29 modulates PIN-FORMED polarity and Arabidopsis development via its own phosphorylation code.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyodong Lee,Anindya Ganguly,Song Baik,Hyung-Taeg ChoPubMed 34402905
- Science signaling 14:2021Dysregulation of the AP2M1 phosphorylation cycle by LRRK2 impairs endocytosis and leads to dopaminergic neurodegeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQinfang Liu,Judith Bautista-Gomez,Daniel A Higgins,Jianzhong Yu,Yulan XiongPubMed 34315807
- Molecular & cellular proteomics : MCP 20:1001022021Nuclear Phosphatidylinositol 3,4,5-Trisphosphate Interactome Uncovers an Enrichment in Nucleolar Proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFatemeh Mazloumi Gavgani,Malene Skuseth Slinning,Andrea Papdiné Morovicz,Victoria Smith Arnesen,Diana C Turcu,Sandra Ninzima,Clive S D'Santos,Aurélia E LewisPubMed 34048982
- Cell death and differentiation 28:1563-15782020The stress-responsive kinase DYRK2 activates heat shock factor 1 promoting resistance to proteotoxic stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRita Moreno,Sourav Banerjee,Angus W Jackson,Jean Quinn,Gregg Baillie,Jack E Dixon,Albena T Dinkova-Kostova,Joanne Edwards,Laureano de la VegaPubMed 33268814
- Developmental cell 50:509-524.e102019PI31 Is an Adaptor Protein for Proteasome Transport in Axons and Required for Synaptic Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Liu et. al.PubMed 31327739
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7
Constituents: 15% Sodium chloride, 3% Triton X-100, 3% Sodium deoxycholate, 0.3% Tris HCl- Form
- Liquid
- Clonality
- Polyclonal
Reactivity data
Select an application| ELISA | WB | |
|---|---|---|
| Tag | Tested | Expected |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/10000.00000 - 1/25000.00000 | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Alternative names
Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST
Recommended products
Anti-GST antibody - HRP conjugated (ab3416) is a rabbit polyclonal antibody detecting GST in Western Blot, ELISA.
- Over 30 publications
- Trusted since 2003
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7
Constituents: 15% Sodium chloride, 3% Triton X-100, 3% Sodium deoxycholate, 0.3% Tris HCl- Form
- Liquid
- Clonality
- Polyclonal
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Immunoaffinity purified using GST immobilized on a solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
HRP Anti-GST antibody (ab3416) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), ELISA.
Trusted by the scientific community
Anti-GST HRP (ab3416) was first used in a scientific publication in 2003 and has been cited over 30 times in peer-reviewed journals.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.
- Biological function summary
GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.
- Pathways
GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.
- Associated diseases and disorders
GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
This image is courtesy of an anonymous customer review.ELISA - HRP Anti-GST antibody (ab3416)
Graph of ELISA measuring levels of bound GST-tagged mosquito proteins. 5g of target protein was coated onto an ELISA plate and incubated overnight at 4°C. The plate was washed and GST-tagged mosquito proteins were incubated for an hour at RT. The proteins were washed off, GST-HRP antibody (ab3416) was diluted 1/5000 in 1%BSA in PBS and added for 30 min at RT. The plate was washed and TMB substrate was added for 20 min at RT. Stop solution was added and the O.D. of the wells read at 450 nm.
This image is courtesy of an anonymous customer review.ELISA - HRP Anti-GST antibody (ab3416)
Graph of ELISA measuring levels of bound GST-tagged mosquito proteins. 5g of target protein was coated onto an ELISA plate and incubated overnight at 4°C. The plate was washed and GST-tagged mosquito proteins were incubated for an hour at RT. The proteins were washed off, GST-HRP antibody (ab3416) was diluted 1/5000 in 1%BSA in PBS and added for 30 min at RT. The plate was washed and TMB substrate was added for 20 min at RT. Stop solution was added and the O.D. of the wells read at 450 nm.
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