AB58626

HRP Anti-GST antibody

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(2 Reviews)

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(19 Publications)

Goat Polyclonal GST antibody - conjugated to HRP. Suitable for ELISA, WB, IHC-P and reacts with Tag samples. Cited in 19 publications.

View Alternative Names

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

1 Images

ELISA - HRP Anti-GST antibody (AB58626)

ELISA

AbReview14113****

ELISA - HRP Anti-GST antibody (AB58626)

ab58626 staining GST in by ELISA. The sample was blocked in 1% milk for 1 hour at 18°C. The primary was diluted at 1/2000 in PBS-Tween20 buffer and incubated with sample for 1 hour at 18°C.

This image is courtesy of an anonymous Abreview

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

No

Applications

WB, ELISA, IHC-P

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Purification notes

Anti-GST HRP Conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer.

Storage buffer

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.

Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

See full target information Glutathione S-transferase class-mu 26 kDa isozyme

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107770 PubMed39270823

2024

Evolutionary and molecular basis of ADP-ribosylation reversal by zinc-dependent macrodomains.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Ariza,Qiang Liu,Nathan P Cowieson,Ivan Ahel,Dmitri V Filippov,Johannes Gregor Matthias Rack

Protein & cell 11:894-914 PubMed32562145

2020

TRIM35 mediates protection against influenza infection by activating TRAF3 and degrading viral PB2.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Sun,Li Jiang,Miaomiao Ye,Yihan Wang,Guangwen Wang,Xiaopeng Wan,Yuhui Zhao,Xia Wen,Libin Liang,Shujie Ma,Liling Liu,Zhigao Bu,Hualan Chen,Chengjun Li

Journal of peptide science : an official publication of the European Peptide Society 25:e3203 PubMed31347248

2019

Inhibition of heme sequestration of histidine-rich protein 2 using multiple epitope-targeted peptides.

Applications

Unspecified application

Species

Unspecified reactive species

JingXin Liang,David N Bunck,Anvita Mishra,Sunga Hong,Matthew N Idso,James R Heath

The Biochemical journal 475:3827-3846 PubMed30373764

2018

Characterization of TCDD-inducible poly-ADP-ribose polymerase (TIPARP/ARTD14) catalytic activity.

Applications

Unspecified application

Species

Unspecified reactive species

Alvin Gomez,Christian Bindesbøll,Somisetty V Satheesh,Giulia Grimaldi,David Hutin,Laura MacPherson,Shaimaa Ahmed,Laura Tamblyn,Tiffany Cho,Hilde Irene Nebb,Anders Moen,Jan Haug Anonsen,Denis M Grant,Jason Matthews

Nature communications 8:945 PubMed29038422

2017

TRIM29 promotes DNA virus infections by inhibiting innate immune response.

Applications

Unspecified application

Species

Unspecified reactive species

Junji Xing,Ao Zhang,Hua Zhang,Jin Wang,Xian Chang Li,Mu-Sheng Zeng,Zhiqiang Zhang

The Plant cell 29:2233-2248 PubMed28855332

2017

GENERAL CONTROL NONREPRESSIBLE4 Degrades 14-3-3 and the RIN4 Complex to Regulate Stomatal Aperture with Implications on Nonhost Disease Resistance and Drought Tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Amita Kaundal,Vemanna S Ramu,Sunhee Oh,Seonghee Lee,Bikram Pant,Hee-Kyung Lee,Clemencia M Rojas,Muthappa Senthil-Kumar,Kirankumar S Mysore

Nature communications 7:13889 PubMed28004827

2016

HS1BP3 negatively regulates autophagy by modulation of phosphatidic acid levels.

Applications

Unspecified application

Species

Unspecified reactive species

Petter Holland,Helene Knævelsrud,Kristiane Søreng,Benan J Mathai,Alf Håkon Lystad,Serhiy Pankiv,Gunnveig T Bjørndal,Sebastian W Schultz,Viola H Lobert,Robin B Chan,Bowen Zhou,Knut Liestøl,Sven R Carlsson,Thomas J Melia,Gilbert Di Paolo,Anne Simonsen

Nature immunology 17:1373-1380 PubMed27695001

2016

Identification of a role for TRIM29 in the control of innate immunity in the respiratory tract.

Applications

Unspecified application

Species

Unspecified reactive species

Junji Xing,Leiyun Weng,Bin Yuan,Zhuo Wang,Li Jia,Rui Jin,Hongbo Lu,Xian Chang Li,Yong-Jun Liu,Zhiqiang Zhang

The Biochemical journal 473:899-910 PubMed26814197

2016

TCDD-inducible poly-ADP-ribose polymerase (TIPARP/PARP7) mono-ADP-ribosylates and co-activates liver X receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Bindesbøll,Susanna Tan,Debbie Bott,Tiffany Cho,Laura Tamblyn,Laura MacPherson,Line Grønning-Wang,Hilde Irene Nebb,Jason Matthews

Journal of lipid research 56:771-85 PubMed25724563

2015

Liver X receptor regulates hepatic nuclear O-GlcNAc signaling and carbohydrate responsive element-binding protein activity.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Bindesbøll,Qiong Fan,Rikke C Nørgaard,Laura MacPherson,Hai-Bin Ruan,Jing Wu,Thomas Å Pedersen,Knut R Steffensen,Xiaoyong Yang,Jason Matthews,Susanne Mandrup,Hilde I Nebb,Line M Grønning-Wang

View all publications

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Product promise

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