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Mouse Monoclonal HA tag antibody - conjugated to HRP. Suitable for WB and reacts with Tag samples. Cited in 2 publications.


Images

Western blot - HRP Anti-HA tag antibody [2-2.2.14] (AB173826), expandable thumbnail
  • Western blot - HRP Anti-HA tag antibody [2-2.2.14] (AB173826), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

HRP

Storage buffer

pH: 7.4

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Tag
Tested

Tested
Tested

Species

Tag

Dilution info

1/500.00000 - 1/2000.00000

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

1 product for Alternative Version

Target data

Alternative names

HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain

Recommended products

Mouse Monoclonal HA tag antibody - conjugated to HRP. Suitable for WB and reacts with Tag samples. Cited in 2 publications.

Key facts

Isotype

IgG1

Conjugation

HRP

Form

Liquid

Clonality

Monoclonal

Clone number

2-2.2.14

Purification technique

Affinity purification Protein G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Product promise

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2 product images

  • Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826), expandable thumbnail

    Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826)

    Note: the predicted MW indicated is for the HA tag.

    All lanes: Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826) at 1/1000 dilution

    Lane 1: E. coli lysate containing a multi-epitope tagged protein at 1 µg

    Lane 2: E. coli lysate containing a multi-epitope tagged protein at 0.5 µg

    Lane 3: E. coli lysate containing a multi-epitope tagged protein at 0.25 µg

    Lane 4: E. coli lysate containing a multi-epitope tagged protein at 0.125 µg

    Lane 5: E. coli lysate containing a multi-epitope tagged protein at 0.05 µg

    Developed using the ECL technique.

    Predicted band size: 64 kDa

  • Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826), expandable thumbnail

    Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826)

    Antibody specificity demonstrated by detection of different targets fused to HA tag in transiently transfected lysates tested. Relative detection of HA tag was observed across different proteins fused with HA tag in HA-H3-FLAG (Lane 3-6) and FLAG-p65-HA (Lane 7), using ab173826 in Western Blot. Positope lacks HA tag, which has been used as a negative control for HA tag detection. No cross-reactivity was seen with Myc or V5-tagged p65 expressing lysate.

    All lanes: Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826) at 0.2 µg/mL

    Lane 1: HEK-293E lysates: untransfected at 40 µg

    Lane 2: HEL-293E lysates: empty vector control at 40 µg

    Lane 3: HEK-293E lysates: HA-H3-FLAG transfected at 40 µg

    Lane 4: HEK-293E lysates: HA-H3-FLAG transfected at 20 µg

    Lane 5: HEK-293E lysates: HA-H3-FLAG transfected at 10 µg

    Lane 6: HEK-293E lysates: HA-H3-FLAG transfected at 5 µg

    Lane 7: HEK-293E lysates: FLAG-p65-HA transfected at 40 µg

    Lane 8: Myc-p65-V5-tagged p65 expressing lysate at 40 µg

    Lane 9: Negative control: Positope at 0.025 µg

    Predicted band size: 64 kDa

    Observed band size: ~19 kDa, ~67 kDa

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Product protocols

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