HRP Anti-HA tag antibody [2-2.2.14]

• WB

Supplier Data

Western blot - HRP Anti-HA tag antibody [2-2.2.14] (AB173826)

Note : the predicted MW indicated is for the HA tag.

All lanes:

Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826) at 1/1000 dilution

Lane 1:

E. coli lysate containing a multi-epitope tagged protein at 1 µg

Lane 2:

E. coli lysate containing a multi-epitope tagged protein at 0.5 µg

Lane 3:

E. coli lysate containing a multi-epitope tagged protein at 0.25 µg

Lane 4:

E. coli lysate containing a multi-epitope tagged protein at 0.125 µg

Lane 5:

E. coli lysate containing a multi-epitope tagged protein at 0.05 µg

Predicted band size: 64 kDa

true

• WB

Supplier Data

Western blot - HRP Anti-HA tag antibody [2-2.2.14] (AB173826)

Antibody specificity demonstrated by detection of different targets fused to HA tag in transiently transfected lysates tested. Relative detection of HA tag was observed across different proteins fused with HA tag in HA-H3-FLAG (Lane 3-6) and FLAG-p65-HA (Lane 7), using ab173826 in Western Blot. Positope lacks HA tag, which has been used as a negative control for HA tag detection. No cross-reactivity was seen with Myc or V5-tagged p65 expressing lysate.

All lanes:

Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826) at 0.2 µg/mL

Lane 1:

HEK-293E lysates: untransfected at 40 µg

Lane 2:

HEL-293E lysates: empty vector control at 40 µg

Lane 3:

HEK-293E lysates: HA-H3-FLAG transfected at 40 µg

Lane 4:

HEK-293E lysates: HA-H3-FLAG transfected at 20 µg

Lane 5:

HEK-293E lysates: HA-H3-FLAG transfected at 10 µg

Lane 6:

HEK-293E lysates: HA-H3-FLAG transfected at 5 µg

Lane 7:

HEK-293E lysates: FLAG-p65-HA transfected at 40 µg

Lane 8:

Myc-p65-V5-tagged p65 expressing lysate at 40 µg

Lane 9:

Negative control: Positope at 0.025 µg

Predicted band size: 64 kDa

Observed band size: ~19 kDa,~67 kDa

false