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AB20629

HRP Anti-Helicobacter pylori antibody

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(3 Publications)

Rabbit Polyclonal Helicobacter pylori antibody - conjugated to HRP. Suitable for ELISA, IHC-FoFr and reacts with Helicobacter pylori samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing Helicobacter pylori protein.

View Alternative Names

H. pylori

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Helicobacter pylori

Applications

IHC-FoFr, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Helicobacter pylori": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/20 - 1/1000", "ELISA-species-notes": "<p></p>", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "1/20 - 1/200", "IHCFoFr-species-notes": "<p></p>" } } }
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Product details

Estimated molar HRP:IgG substitution is 2-3.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Source: whole cell lysate. Protein A chromatography purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent.
Storage buffer
Preservative: 0.002% Thimerosal (merthiolate) Constituents: 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Helicobacter pylori often called H. pylori is a gram-negative spiral-shaped bacterium with a mass of about 3.39 megadaltons. This organism colonizes the human gastric epithelium where it survives in the acidic environment of the stomach. The bacterium expresses urease which breaks down urea to ammonia and carbon dioxide helping neutralize gastric acid. It attaches to the gastric mucosa using flagella for motility and adheres to host cells via adhesins. Researchers utilize specific staining methods such as the Helicobacter pylori stain and tools like anti-Helicobacter antibodies to detect and study this pathogen.
Biological function summary

Helicobacter pylori plays a significant role in gastric health by disrupting mucosal integrity. It engages in the secretion of virulence factors like CagA and VacA leading to changes in cellular signaling and inflammation. H. pylori does not operate as part of a large complex but its interactions with the gastric mucosa have substantial effects. Detection and diagnosis often employ tools like H. pylori IHC and H. pylori antibody kits which target the bacterium's proteins to confirm infection presence.

Pathways

H. pylori infection activates signaling routes including the NF-κB pathway and the MAPK pathway in gastric epithelial cells. These pathways contribute to inflammatory responses and immune cell activation leading to mucosal damage. Within these pathways proteins like tumor necrosis factor-alpha (TNF-α) and interleukin-8 (IL-8) play roles in mediating inflammation initiated by the bacterial colonization of the gastric lining.

Chronic H. pylori infection has strong associations with gastric ulcers and gastric cancer. The bacterium promotes persistent inflammation and cellular damage that can progress to malignancy. H. pylori-related disorders are linked with proteins such as CagA a known oncogenic factor that interferes with host cell signaling and promotes carcinogenesis. Detection and specific targeting by Helicobacter pylori antibodies play important roles in managing and studying these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Helicobacter pylori
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 14:1335401 PubMed38835368

2024

A glutamatergic biomarker panel enables differentiating Grade 4 gliomas/astrocytomas from brain metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Falko Lange,Richard Gade,Anne Einsle,Katrin Porath,Gesine Reichart,Claudia Maletzki,Björn Schneider,Christian Henker,Daniel Dubinski,Michael Linnebacher,Rüdiger Köhling,Thomas M Freiman,Timo Kirschstein

iScience 27:109778 PubMed38746665

2024

A cingulate-hippocampal circuit mediates early depressive-like behavior in the mouse model of Alzheimer disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yanbing Chen,Huimin Peng,Kai Zhuang,Wenting Xie,Chenli Li,Meiqin Chen,Jin Xue,Xiaoting Huang,Tingting Zou,Hao Sun,Aiyu Lei,Ya Wang,Dan Can,Huifang Li,Tifei Yuan,Jie Zhang

Scientific reports 9:13859 PubMed31554912

2019

Impact of conjugation strategies for targeting of antibodies in gold nanoparticles for ultrasensitive detection of 17β-estradiol.

Applications

Unspecified application

Species

Unspecified reactive species

Jairo Pinto Oliveira,Adilson Ribeiro Prado,Wanderson Juvencio Keijok,Paulo Wagnner Pereira Antunes,Enrique Ronald Yapuchura,Marco Cesar Cunegundes Guimarães
View all publications
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