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AB198556

HRP Anti-Hemopexin antibody [EPR5610]

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(1 Publication)

Rabbit Recombinant Monoclonal Hemopexin antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Hemopexin, Beta-1B-glycoprotein, HPX

1 Images
Western blot - HRP Anti-Hemopexin antibody [EPR5610] (AB198556)
  • WB

Lab

Western blot - HRP Anti-Hemopexin antibody [EPR5610] (AB198556)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198556 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Hemopexin antibody [EPR5610] (ab198556) at 1/5000 dilution

All lanes:

Human Plasma Total Protein Lysate at 10 µg

Predicted band size: 51 kDa

Observed band size: 73 kDa

true

Exposure time: 20s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5610

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hemopexin also known as HPX is a glycoprotein that transports heme in the blood. It has a molecular weight of approximately 57 kDa and is primarily expressed in the liver. Hemopexin binds to free heme with high affinity which prevents oxidative damage and conserves iron. It transports the heme to hepatocytes for breakdown and recycling protecting cells from iron-mediated toxicity and oxidative stress.
Biological function summary

The hemopexin function relates to protecting tissues from the harmful effects of free heme. Hemopexin does not work as part of a complex in this process but mainly acts independently. Its high binding affinity plays an important role in maintaining heme homeostasis in the plasma and tissues. By controlling heme levels hemopexin supports overall cellular health and function.

Pathways

The hemopexin molecular weight allows it to fit effectively within the heme metabolism and iron recycling pathways. This protein plays a significant role by delivering heme to the liver and facilitating its degradation by heme oxygenase. Hemopexin interacts closely with heme oxygenase and albumin two proteins important for the management of heme and iron levels in the body.

Several hemopexin functions contribute to the understanding of conditions such as hemolytic anemia and acute liver injury. In hemolytic anemia hemopexin helps mitigate the effects of increased heme levels due to excessive red blood cell breakdown. In the context of liver injury the protein assists in the detoxification process and supports hepatic function. Hemopexin-related proteins such as heme oxygenase link these diseases to hemopexin by their roles in managing oxidative stress and heme catabolism.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
See full target information Hemopexin
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of materials science. Materials in medicine 33:4 PubMed34940936

2021

Hydrogel contained valproic acid accelerates bone-defect repair via activating Notch signaling pathway in ovariectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou-Shan Tao,Tian-Lin Li,Hong-Guang Xu,Min Yang
View all publications
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