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AB204463

HRP Anti-Histone H2B antibody [mAbcam 52484]

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(2 Publications )

Anti-Histone H2B antibody [mAbcam 52484] conjugated to HRP - suitable for IHC-P, Western blot, and reacts with human samples. Clone [mAbcam 52484] is a mouse monoclonal antibody.
2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Histone H2B antibody [mAbcam 52484] (AB204463)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Histone H2B antibody [mAbcam 52484] (AB204463)

IHC image of Histone H2B staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution

  1. for 20mins. The section was then incubated with ab204463, 1/1000 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Western blot - HRP Anti-Histone H2B antibody [mAbcam 52484] (AB204463)
  • WB

Lab

Western blot - HRP Anti-Histone H2B antibody [mAbcam 52484] (AB204463)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab204463 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Histone H2B antibody [mAbcam 52484] (ab204463) at 1/7500 dilution

Lane 1:

Histone H1 Recombinant Protein at 0.1 µg

Lane 2:

Histone H2A Recombinant Protein at 0.1 µg

Lane 3:

Histone H2B Recombinant Protein at 0.1 µg

Lane 4:

Histone H3 Recombinant Protein at 0.1 µg

Lane 5:

Histone H4 Recombinant Protein at 0.1 µg

Predicted band size: 14 kDa

Observed band size: 17 kDa

false

Exposure time: 5s

  • Unconjugated

    Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade

  • Carrier free

    Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Histone H2B antibody [mAbcam 52484]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Histone H2B antibody [mAbcam 52484]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam 52484

Isotype

IgG1

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/7500", "WB-species-notes": "<p></p>" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
See full target information H2BC12

Additional targets

,H2BC17,H2BC13,H2BC18,H2BC21 acetyl K15,,,H2BC11,H2BC4,,,H2BC3,H2BC12L

Alternative Names

H2BFT, HIRIP1, HIST1H2BK, H2BC12, Histone H2B type 1-K, H2B K, HIRA-interacting protein 1, H2BFF, HIST1H2BB, H2BC3, Histone H2B type 1-B, H2B-clustered histone 3, Histone H2B.1, Histone H2B.f, H2B/f, H2BFL, HIST1H2BC, H2BC6, H2BFH, HIST1H2BE, H2BC7, H2BFG, HIST1H2BF, H2BC8, H2BFA, HIST1H2BG, H2BC10, H2BFK, HIST1H2BI, H2BC4, Histone H2B type 1-C/E/F/G/I, Histone H2B.1 A, Histone H2B.a, Histone H2B.g, Histone H2B.h, Histone H2B.k, Histone H2B.l, H2B/a, H2B/g, H2B/h, H2B/k, H2B/l, H2BFC, HIST1H2BL, H2BC13, Histone H2B type 1-L, Histone H2B.c, H2B/c, H2BFS, H2BS1, H2BC12L, Histone H2B type F-S, H2B-clustered histone 12 like, H2B.S histone 1, Histone H2B.s, H2B/s, H2BFQ, HIST2H2BE, H2BC21, Histone H2B type 2-E, H2B-clustered histone 21, Histone H2B-GL105, Histone H2B.q, H2B/q, H2BK15ac, H2BFR, HIST1H2BJ, H2BC11, Histone H2B type 1-J, Histone H2B.1, Histone H2B.r, H2B/r, H2BFD, HIST1H2BN, H2BC15, Histone H2B type 1-N, Histone H2B.d, H2B/d, H2BFE, HIST1H2BM, H2BC14, Histone H2B type 1-M, Histone H2B.e, H2B/e, H2BFH, H2BFN, HIST1H2BO, H2BC17, Histone H2B type 1-O, H2B-clustered histone 17, Histone H2B.2, Histone H2B.n, H2B/n, H2BFB, HIRIP2, HIST1H2BD, H2BC5, Histone H2B type 1-D, H2B-clustered histone 5, HIRA-interacting protein 2, Histone H2B.1 B, Histone H2B.b, H2B/b, HIST2H2BF, H2BC18, Histone H2B type 2-F, H2B-clustered histone 18, H2BU1, HIST3H2BB, H2BC26, Histone H2B type 3-B, H2B type 12, H2B-clustered histone 26, H2B.U histone 1, H2BFJ, HIST1H2BH, H2BC9, Histone H2B type 1-H, H2B-clustered histone 9, Histone H2B.j, H2B/j
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 15:151-164 PubMed36777827

2023

Protocatechuic acid, the main effective monomer in Wuqi Powder, can inhibit gastric ulcers induced by acetic acid and .

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Wang,Liang Zheng

Journal of experimental & clinical cancer research : CR 41:274 PubMed36109793

2022

RETSAT associates with DDX39B to promote fork restarting and resistance to gemcitabine based chemotherapy in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiu Tu,Xiuyun Liu,Xiaoqing Yao,Ruixue Li,Gaojing Liu,Honglv Jiang,Kaiqin Li,Qiongfang Chen,Xiaoyan Huang,Qing Chang,Guoqiang Xu,Hong Zhu,Peng Shi,Bo Zhao
View all publications
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Product promise

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