HRP Anti-Histone H3 antibody - Nuclear Loading Control

• WB

Project

Western blot - HRP Anti-Histone H3 antibody - Nuclear Loading Control (AB21054)

All lanes:

Western blot - HRP Anti-Histone H3 antibody - Nuclear Loading Control (ab21054) at 1/5000 dilution

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

Yeast S.cerevisiae Whole Cell Lysate at 10 µg

Lane 3:

Drosophila Embryo Nuclear Extract at 20 µg

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Histone H3 antibody - Nuclear Loading Control (AB21054)

IHC image of Histone H3 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21054, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Histone H3 antibody - Nuclear Loading Control (AB21054)

IHC image of Histone H3 staining in a section of formalin-fixed paraffin-embedded normal human cervix tissue*, performed on a Leica BOND^TM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab21054, 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre