AB62168

HRP Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220]

Name: Anti-Histone H3 (di methyl K9) HRP antibody [mAbcam 1220] (ab62168) | Abcam
Brand: Abcam Limited
SKU: AB62168

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(1 Publication)

Mouse Monoclonal H3 di methyl K9 antibody - conjugated to HRP. Suitable for WB and reacts with Cow samples. Cited in 1 publication.

View Alternative Names

Histone H3.1, H3K9me2, H3K9me, H3K9

1 Images

Western blot - HRP Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] (AB62168)

All lanes:

Western blot - HRP Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] (ab62168) at 1/20000 dilution

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified ) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab2903'>ab2903</a>)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k4-peptide-ab1342'>ab1342</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Lane 9:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k27-peptide-ab1782'>ab1782</a>)

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220]
Unconjugated

Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam 1220

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Cow

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2026, QC testing of replenishment batches of this primary conjugate changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, the replenishment lots are subjected to bioanalytical testing only. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Arabidopsis thaliana": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Caenorhabditis elegans": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000 - 1/50000", "WB-species-notes": "<p></p>" }, "Drosophila melanogaster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rice": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Saccharomyces cerevisiae": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Schizosaccharomyces pombe": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information Histone H3.1 di methyl K9

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:4758 PubMed38902234

2024

Gliovascular transcriptional perturbations in Alzheimer's disease reveal molecular mechanisms of blood brain barrier dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Özkan İş,Xue Wang,Joseph S Reddy,Yuhao Min,Elanur Yilmaz,Prabesh Bhattarai,Tulsi Patel,Jeremiah Bergman,Zachary Quicksall,Michael G Heckman,Frederick Q Tutor-New,Birsen Can Demirdogen,Launia White,Shunsuke Koga,Vincent Krause,Yasuteru Inoue,Takahisa Kanekiyo,Mehmet Ilyas Cosacak,Nastasia Nelson,Annie J Lee,Badri Vardarajan,Richard Mayeux,Naomi Kouri,Kaancan Deniz,Troy Carnwath,Stephanie R Oatman,Laura J Lewis-Tuffin,Thuy Nguyen,Minerva M Carrasquillo,Jonathan Graff-Radford,Ronald C Petersen,Clifford R Jr Jack,Kejal Kantarci,Melissa E Murray,Kwangsik Nho,Andrew J Saykin,Dennis W Dickson,Caghan Kizil,Mariet Allen,Nilüfer Ertekin-Taner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220]

Western blot - HRP Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] (AB62168)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise