JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB305721

HRP Anti-HNF-4-alpha antibody [EPR3648]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal HNF-4-alpha antibody - conjugated to HRP.

View Alternative Names

HNF4, NR2A1, TCF14, HNF4A, Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, Transcription factor HNF-4, TCF-14

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-HNF-4-alpha antibody [EPR3648]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-HNF-4-alpha antibody [EPR3648]

  • 578 PE

    PE Anti-HNF-4-alpha antibody [EPR3648]

  • 660 APC

    APC Anti-HNF-4-alpha antibody [EPR3648]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-HNF-4-alpha antibody [EPR3648]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-HNF-4-alpha antibody [EPR3648]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-HNF-4-alpha antibody [EPR3648]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-HNF-4-alpha antibody [EPR3648]

  • Unconjugated

    Anti-HNF-4-alpha antibody [EPR3648]

  • Carrier free

    Anti-HNF-4-alpha antibody [EPR3648] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3648

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HNF-4-alpha also known as hepatocyte nuclear factor 4-alpha (HNF4A) is a nuclear protein that functions as a transcription factor. It plays an important role in regulating gene expression in the liver kidney and intestine. HNF-4-alpha has a molecular mass of about 54 kDa and is expressed in tissues where it maintains cellular differentiation and metabolic homeostasis. In cellular assays such as ELISA and CHIP detection of HNF-4-alpha provides insights into its expression levels and activity in various biological contexts.
Biological function summary

HNF-4-alpha influences the expression of genes involved in liver and pancreatic function. It acts as a transcriptional regulator that controls fatty acid oxidation gluconeogenesis and insulin secretion. HNF-4-alpha interacts with other proteins to form transcriptional complexes that fine-tune the expression of metabolic enzymes and transport proteins. This protein is key to ensuring that metabolic genes are expressed in a coordinated manner which is essential for maintaining normal metabolic processes.

Pathways

HNF-4-alpha participates in the insulin signaling and lipid metabolism pathways. Through these pathways it interacts with other transcription factors such as HNF-1-alpha and PPAR-alpha which coordinate the regulation of genes involved in glucose metabolism and fatty acid oxidation. HNF-4-alpha plays a critical role by modulating the expression of enzymes like gluconeogenic enzymes in response to metabolic needs which connects it to key metabolic pathways influencing overall energy homeostasis.

HNF-4-alpha is linked to maturity-onset diabetes of the young (MODY1) and certain types of liver diseases. Mutations or dysregulation in HNF-4-alpha can affect the expression of genes essential for insulin production and glucose metabolism leading to disorders like diabetes. Additionally HNF-4-alpha interacts with other proteins and transcription factors implicated in these conditions such as HNF-1-alpha. Understanding the role of HNF-4-alpha in these diseases can provide insights into potential therapeutic targets and intervention strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes (PubMed : 30597922). Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells (PubMed : 30530698).
See full target information HNF4A
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB65333

Glucose Assay Kit

Functional Studies - Glucose Assay Kit (AB65333)

  • 0
  • 0
Primary Antibodies

AB209478

PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Recombinant

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

  • 0
  • 0
Primary Antibodies

AB198337

Alexa Fluor® 647 Anti-RAB7 antibody [EPR7589] - Late Endosome Marker

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-RAB7 antibody [EPR7589] - Late Endosome Marker (AB198337)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB281764

Alexa Fluor® 555 Anti-CD16 antibody [EPR16784]

RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD16 antibody [EPR16784] (AB281764)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com