JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB197721

HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal HP1 alpha antibody - conjugated to HRP. Heterochromatin marker. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HP1A, CBX5, Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha

2 Images
Western blot - HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (AB197721)
  • WB

Lab

Western blot - HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (AB197721)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197721 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab197721) at 1/5000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 5:

Hap1 WT at 20 µg

Lane 6:

HP1 alpha knockout HAP1 cell lysate at 20 µg

Predicted band size: 22 kDa

Observed band size: 28 kDa

true

Exposure time: 16min

Western blot - HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (AB197721)
  • WB

Lab

Western blot - HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (AB197721)

ab197721 was shown to specifically react with HP1 alpha in wild-type HAP1 cells as signal was lost in CBX5 (HP1 alpha) knockout cells. Wild-type and CBX5 (HP1 alpha) knockout samples were subjected to SDS-PAGE. ab197721 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

All lanes:

Western blot - HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab197721) at 1/5000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

CBX5 (HP1 alpha) knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 22 kDa

false

Exposure time: 20min

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

  • 578 PE

    PE Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

  • Unconjugated

    Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

  • Carrier free

    Anti-HP1 alpha antibody [EPR5777] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5777

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HP1 alpha also known as HP1a or HP1 protein is a well-studied member of the heterochromatin protein 1 family. It plays a mechanical role in chromatin organization and gene regulation by binding to histone H3 when it's methylated at lysine 9 (H3K9me). This protein has a molecular mass of approximately 22-25 kDa. HP1 alpha is commonly expressed in the nuclei of eukaryotic cells where it localizes to heterochromatin regions and influences chromatin compaction.
Biological function summary

Heterochromatin protein 1 alpha acts as a modulator of gene silencing. It is part of a larger protein complex that contains various chromatin modifiers responsible for maintaining the silent state of certain genes. By mediating these interactions HP1 alpha helps regulate transcription replication timing and DNA repair processes. This regulation is essential for the structural maintenance of heterochromatin and plays a part in chromosomal stability.

Pathways

HP1 alpha is integral in the epigenetic regulation pathway along with other well-known proteins including SUV39H1 and SUV39H2 which are histone methyltransferases. HP1 alpha also links to the DNA damage response pathway where it recruits repair factors to maintain genomic integrity. Through these pathways HP1 alpha interacts closely with proteins like BRCA1 emphasizing its role in cellular homeostasis and response to DNA damage.

Researchers associate HP1 alpha with cancer and developmental disorders. Alterations in HP1 alpha expression or function can lead to disruptions in chromatin organization contributing to oncogenesis by affecting tumor suppressor genes. Furthermore HP1 alpha links to disorders like ICF syndrome through its relationship with the DNA methylation process where it interacts with proteins such as DNMT3B. Understanding these connections provides insights into therapeutic approaches targeting epigenetic modifications in disease contexts.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph) (PubMed : 19783980). May contribute to the association of heterochromatin with the inner nuclear membrane by interactions with the lamin-B receptor (LBR) (PubMed : 19783980). Involved in the formation of kinetochore through interaction with the MIS12 complex subunit NSL1 (PubMed : 19783980, PubMed : 20231385). Required for the formation of the inner centromere (PubMed : 20231385).
See full target information CBX5
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 151: PubMed39431301

2024

The GTPase RAB6 is required for stem cell maintenance and cell migration in the gut epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Pierre Simonin,Gehenna Lobo Guerrero,Sabine Bardin,Ram Venkata Gannavarapu,Denis Krndija,Joseph Boyd,Stephanie Miserey,Danijela Matic Vignjevic,Bruno Goud
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB133577

Anti-HP1 alpha antibody [EPR5776]

RabMAb|Recombinant|Advanced Validation

Flow Cytometry (Intracellular) - Anti-HP1 alpha antibody [EPR5776] (AB133577)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB18207

Anti-beta III Tubulin antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - Neuronal Marker (AB18207)

  • 5
  • 67
Primary Antibodies

AB177790

Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606]

RabMAb|Advanced Validation|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (AB177790)

  • 5
  • 2
Primary Antibodies

AB62341

Anti-RFP antibody

Lab Essentials

Western blot - Anti-RFP antibody (AB62341)

  • 4
  • 23

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com