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Rabbit Recombinant Monoclonal ILF3 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

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Images

Western blot - HRP Anti-ILF3 antibody [EPR3626] (AB206250), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-ILF3 antibody [EPR3626] (AB206250), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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1 product for Alternative Version

Target data

Function

RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed:28625552). As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:14731398). Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed:21123651, PubMed:34110282). Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs (PubMed:21123651, PubMed:28625552). (Microbial infection) Plays a positive role in HIV-1 virus production by binding to and thereby stabilizing HIV-1 RNA, together with ILF3.

Alternative names

DRBF, MPHOSPH4, NF90, ILF3, Interleukin enhancer-binding factor 3, Double-stranded RNA-binding protein 76, M-phase phosphoprotein 4, Nuclear factor associated with dsRNA, Nuclear factor of activated T-cells 90 kDa, Translational control protein 80, DRBP76, MPP4, NFAR, NF-AT-90, TCP80

Recommended products

Rabbit Recombinant Monoclonal ILF3 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3626
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ILF3 protein also known as Interleukin enhancer-binding factor 3 plays an important role in cellular mechanisms. It has a molecular mass of approximately 90 kDa and is expressed broadly in various tissues such as the liver kidney and spleen. ILF3 is part of the double-stranded RNA-binding proteins and it engages in regulating RNA metabolism processes. Scientists also refer to ILF3 as NF90 and it is known for its function in modulating gene expression by interacting with RNA.

Biological function summary

ILF3 interacts with RNA and proteins within the cell to control the stability and translation of mRNA. It acts as a component of various ribonucleoprotein complexes indicating its role in post-transcriptional regulation. ILF3 can bind to double-stranded RNA and single-stranded RNA motifs affecting the proliferation and differentiation of cells in which it is expressed. It also contributes significantly in cellular stress responses impacting how cells respond to environmental changes.

Pathways

ILF3 is involved in important cellular pathways such as RNA processing and antiviral defense mechanisms. In the RNA processing pathway ILF3 associates with proteins such as Drosha impacting microRNA biogenesis. Additionally in antiviral responses it identifies and binds viral RNAs initiating defensive cellular responses. These pathways position ILF3 as integral to maintaining cellular homeostasis during stress and infection.

Associated diseases and disorders

ILF3 has connections to cancer and viral infections. Aberrations in ILF3 expression or function are observed in certain cancers where it influences tumor progression by interacting with oncogenic pathways. In viral infections like those caused by hepatitis C virus ILF3 contributes to host defense by modulating immune responses. It interacts with proteins like RIG-I in the context of viral infections facilitating antiviral signaling pathways to control disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - HRP Anti-ILF3 antibody [EPR3626] (ab206250), expandable thumbnail

    Western blot - HRP Anti-ILF3 antibody [EPR3626] (ab206250)

    Blocking buffer: 3% Milk

    Gel type: MOPS

    Observed band size: 95 kDa

    Exposure Time: 2 minutes

    All lanes: Western blot - HRP Anti-ILF3 antibody [EPR3626] (ab206250) at 1/5000 dilution

    Lane 1: Raji Whole Cell lysate at 10 µg

    Lane 2: HeLa Whole Cell lysate at 10 µg

    Lane 3: K562 Whole Cell lysate at 10 µg

    Predicted band size: 95 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-ILF3 antibody [EPR3626] (ab206250), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-ILF3 antibody [EPR3626] (ab206250)

    IHC image of ILF3 staining in a section of formalin-fixed paraffin-embedded normal human kidney*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab206250, 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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