Rabbit Recombinant Monoclonal ILF3 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
Images
![Western blot - HRP Anti-ILF3 antibody [EPR3626] (AB206250), expandable thumbnail](https://content.abcam.com/products/images/hrp-ilf3-antibody-epr3626-ab206250--western-blot-img78251.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-ILF3 antibody [EPR3626] (AB206250)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-ILF3 antibody [EPR3626] (AB206250), expandable thumbnail](https://content.abcam.com/products/images/hrp-ilf3-antibody-epr3626-ab206250--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81164.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-ILF3 antibody [EPR3626] (AB206250)")
Publications
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- Molecules (Basel, Switzerland) 26:2021Multiplexed In Situ Protein Profiling with High-Performance Cleavable Fluorescent Tyramide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThai Pham,Renjie Liao,Joshua Labaer,Jia GuoPubMed 33921211
- Frontiers in cell and developmental biology 8:6146242021Highly Sensitive and Multiplexed Protein Imaging With Cleavable Fluorescent Tyramide Reveals Human Neuronal Heterogeneity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRenjie Liao,Manas Mondal,Christopher D Nazaroff,Diego Mastroeni,Paul D Coleman,Joshua Labaer,Jia GuoPubMed 33585449
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
1 products for Alternative Version
Target data
Function
RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed:28625552). As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:14731398). Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed:21123651). Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs (PubMed:21123651, PubMed:28625552).
Alternative names
Interleukin enhancer-binding factor 3, Double-stranded RNA-binding protein 76, M-phase phosphoprotein 4, Nuclear factor associated with dsRNA, Nuclear factor of activated T-cells 90 kDa, Translational control protein 80, DRBP76, MPP4, NFAR, NF-AT-90, TCP80, NF90, MPHOSPH4, DRBF, ILF3
Recommended products
Rabbit Recombinant Monoclonal ILF3 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
Alternative names
Interleukin enhancer-binding factor 3, Double-stranded RNA-binding protein 76, M-phase phosphoprotein 4, Nuclear factor associated with dsRNA, Nuclear factor of activated T-cells 90 kDa, Translational control protein 80, DRBP76, MPP4, NFAR, NF-AT-90, TCP80, NF90, MPHOSPH4, DRBF, ILF3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR3626
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Stable for 12 months at -20°C, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - HRP Anti-ILF3 antibody [EPR3626] (ab206250), expandable thumbnail](https://content.abcam.com/products/images/hrp-ilf3-antibody-epr3626-ab206250--western-blot-img78251.jpg "Western blot - HRP Anti-ILF3 antibody [EPR3626] (ab206250)")
Western blot - HRP Anti-ILF3 antibody [EPR3626] (ab206250)
Blocking buffer: 3% Milk
Gel type: MOPS
Observed band size: 95 kDa
Exposure Time: 2 minutes
All lanes: Western blot - HRP Anti-ILF3 antibody [EPR3626] (ab206250) at 1/5000 dilution
Lane 1: Raji Whole Cell lysate at 10 µg
Lane 2: HeLa Whole Cell lysate at 10 µg
Lane 3: K562 Whole Cell lysate at 10 µg
Predicted band size: 95 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-ILF3 antibody [EPR3626] (ab206250), expandable thumbnail](https://content.abcam.com/products/images/hrp-ilf3-antibody-epr3626-ab206250--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81164.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-ILF3 antibody [EPR3626] (ab206250)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-ILF3 antibody [EPR3626] (ab206250)
IHC image of ILF3 staining in a section of formalin-fixed paraffin-embedded normal human kidney*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab206250, 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Downloads
Product protocols
- Visit the general protocols
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