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AB208869

HRP Anti-IMP3 antibody [EPR12021]

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(3 Publications)

Rabbit Recombinant Monoclonal IMP3 antibody - conjugated to HRP. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

IMP3, KOC1, VICKZ3, IGF2BP3, Insulin-like growth factor 2 mRNA-binding protein 3, IGF2 mRNA-binding protein 3, IMP-3, IGF-II mRNA-binding protein 3, KH domain-containing protein overexpressed in cancer, VICKZ family member 3, hKOC

2 Images
Western blot - HRP Anti-IMP3 antibody [EPR12021] (AB208869)
  • WB

Unknown

Western blot - HRP Anti-IMP3 antibody [EPR12021] (AB208869)

ab208869 was shown to specifically react with IMP3 in wild-type HAP1 cells as signal was lost in IGF2BP3 (IMP3) knockout cells. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. ab208869 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

All lanes:

Western blot - HRP Anti-IMP3 antibody [EPR12021] (ab208869) at 1/5000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

IGF2BP3 (IMP3) knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 63 kDa

Observed band size: 70 kDa

false

Exposure time: 3min

Western blot - HRP Anti-IMP3 antibody [EPR12021] (AB208869)
  • WB

Lab

Western blot - HRP Anti-IMP3 antibody [EPR12021] (AB208869)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab208869 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-IMP3 antibody [EPR12021] (ab208869) at 1/5000 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Predicted band size: 63 kDa

Observed band size: 70 kDa

true

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12021

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IMP3 also known as IGF2BP3 is an oncofetal protein belonging to the insulin-like growth factor 2 mRNA-binding protein family. It has a molecular weight of around 65 kDa. IMP3 expresses in various tissues including embryonic tissues and several cancer types. In healthy adults the expression is minimal observed mostly in the testis placenta and some parts of the nervous system. It's significance in development and cancer makes it an important target for research.
Biological function summary

IMP3 plays a role in the regulation of mRNA stability localization and translation. It is a part of ribonucleoprotein complexes that facilitate these processes by binding to the 3' untranslated regions of target mRNAs. The protein influences the expression of genes involved in cell growth differentiation and migration. IMP3's ability to modulate mRNA fate contributes to cellular events essential for development and has implications in cancer progression.

Pathways

The involvement of IMP3 is significant in pathways like the insulin-like growth factor (IGF) signaling pathway and epithelial-mesenchymal transition (EMT). Within these pathways IMP3 modulates the activities of proteins such as IGF2 influencing cell proliferation and survival. Its participation in EMT is essential for processes like metastasis in cancer cells linking it to broader biological processes that govern cell movement and identity change.

High IMP3 expression associates with various cancers including pancreatic and ovarian cancers. It has been connected to the progression and poor prognosis of these cancers. In pancreatic cancer IMP3 can interact with proteins like IGF2 enhancing tumor growth and metastasis. This connection makes IMP3 a potential biomarker for cancer diagnostics and therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Preferentially binds to N6-methyladenosine (m6A)-containing mRNAs and increases their stability (PubMed : 29476152). Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Increases MYC mRNA stability by binding to the coding region instability determinant (CRD) and binding is enhanced by m6A-modification of the CRD (PubMed : 29476152). Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.
See full target information IGF2BP3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Sexual medicine 13:qfaf017 PubMed40161546

2025

Icariin inhibits hyperglycemia-induced cell death in penile cavernous tissue and improves erectile function in type 1 diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Haowei Yang,Wenju Xiong,Jun Jiang,Rui Jiang

Cell death & disease 14:581 PubMed37658049

2023

IGF2BP3 promotes the progression of colorectal cancer and mediates cetuximab resistance by stabilizing EGFR mRNA in an mA-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Jie Chen,Hui-Ye Liu,Zhi-Yuan Xiao,Ting Qiu,Dan Zhang,Ling-Jie Zhang,Fang-Yi Han,Guo-Jun Chen,Xue-Mei Xu,Jiong-Hua Zhu,Yan-Qing Ding,Shu-Yang Wang,Ya-Ping Ye,Hong-Li Jiao

International journal of medical sciences 18:792-800 PubMed33437215

2021

Integrated bioinformatics analysis for differentially expressed genes and signaling pathways identification in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

ChenChen Yang,Aifeng Gong
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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