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AB306038

HRP Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] (C-terminal)

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Rabbit Recombinant Monoclonal Indoleamine 2, 3-dioxygenase antibody - conjugated to HRP.

View Alternative Names

IDO, INDO, IDO1, IDO-1

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Indoleamine 2, 3-dioxygenase antibody [SP260]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal

  • 578 PE

    PE Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] (C-terminal)

  • 660 APC

    APC Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] (C-terminal)

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP260

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Indoleamine 2 3-dioxygenase (IDO) also known as IDO1 is an enzyme involved in the catabolism of tryptophan to kynurenine. It weighs approximately 45 kDa and it is an important target in immunoregulatory processes. IDO is highly expressed in antigen-presenting cells such as dendritic cells and macrophages as well as in various tumor cells where it helps modulate immune responses. This enzyme can also be measured by using assays like IDO ELISA.
Biological function summary

IDO plays a role in immune response regulation by degrading tryptophan an essential amino acid needed for T-cell proliferation. The depletion of tryptophan and the accumulation of its metabolites such as kynurenine cause immunosuppressive effects within the tissue microenvironment. While IDO functions mostly as a standalone enzyme its activity influences the cellular surroundings by altering the balance of local immune reactions.

Pathways

IDO integrates into the tryptophan metabolism pathway and is important in the kynurenine pathway. Through this pathway it maintains immune homeostasis and cell defense mechanisms. Proteins like kynureninase and kynurenine 3-monooxygenase also participate in the same metabolic processes which further extend the effects of tryptophan metabolism on immune cell behavior.

IDO is linked with cancer and chronic inflammatory conditions. In cancer elevated IDO expression suppresses anti-tumor immunity aiding tumor cells to evade immune surveillance. It also plays a role in autoimmune disorders by regulating excessive immune activity. During cancer progression IDO often works in conjunction with immune checkpoint proteins like PD-L1 which further enhances its immunosuppressive capabilities within the tumor microenvironment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed : 17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed : 25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed : 25691885). Acts as a suppressor of anti-tumor immunity (PubMed : 14502282, PubMed : 23103127, PubMed : 25157255, PubMed : 25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed : 25691885). Protects the fetus from maternal immune rejection (PubMed : 25691885).
See full target information IDO1
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Complementary Products

Assay Kits

AB235936

Indoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit

Functional Studies - Indoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit (AB235936)

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Assay Kits

AB235936

Indoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit

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Assay Kits

AB245710

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ELISA - Human IDO ELISA Kit (AB245710)

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Product promise

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