AB170326

HRP Anti-Kappa light chain antibody [KT102]

Name: HRP Anti-Kappa light chain antibody [KT102] (ab170326) | Abcam
Brand: Abcam Limited
SKU: AB170326
Price: 520 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Kappa light chain antibody - conjugated to HRP. Suitable for ELISA, WB and reacts with Rat samples. Cited in 1 publication.

View Alternative Names

HCAK 1, IGKCD, Ig kappa chain C region, Immunoglobulin InV, Immunoglobulin KM, Immunoglobulin kappa constant, Immunoglobulin kappa constant region, Immunoglobulin kappa light chain, Immunoglobulin kappa light chain constant region, Kappa 1 immunoglobulin light chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809, kappa light chain

2 Images

Supplier Data

Western blot - HRP Anti-Kappa light chain antibody [KT102] (AB170326)

All lanes:

Western blot - HRP Anti-Kappa light chain antibody [KT102] (ab170326) at 1/1000 dilution

Lane 1:

rat IgM

Lane 2:

rat IgG2c

Lane 3:

rat IgG2b

Lane 4:

rat IgG2a

Lane 5:

rat IgG1

Predicted band size: 25 kDa

false

ELISA - HRP Anti-Kappa light chain antibody [KT102] (AB170326)

ELISA

Supplier Data

ELISA - HRP Anti-Kappa light chain antibody [KT102] (AB170326)

ELISA for crossreactivity to various immunoglobulins.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KT102

Isotype

IgG1

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Rat

Applications

WB, ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "1/2000", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "This dilution works using DAB as substrate. More dilution is needed for ECL system." }, "Mouse": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "1/2000", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "This dilution works using DAB as substrate. More dilution is needed for ECL system." }, "Rat": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/2000", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "This dilution works using DAB as substrate. More dilution is needed for ECL system." }, "Goat": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "1/2000", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "This dilution works using DAB as substrate. More dilution is needed for ECL system." }, "Rabbit": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "1/2000", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "This dilution works using DAB as substrate. More dilution is needed for ECL system." } } }

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Product details

ab170326 can be used for rat IgG isotyping.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab170326 is purified through a Protein A column and labeled with horseradish peroxidase.

Storage buffer

Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The kappa light chain alternatively known as Ig kappa chain is a component of immunoglobulins which are important for immune response. This protein has a molecular mass of roughly 25 kDa. It predominantly expresses in B lymphocytes where it pairs with heavy chains to form antibodies. Its expression is indicative of mature B cell functions serving as a marker for certain types of immune cells. The kappa light chain can also be detected outside of cells circulating in the blood and other bodily fluids.

Biological function summary

Kappa light chains are integral to the structure and function of antibodies. As part of the immunoglobulin molecule these chains contribute to antigen specificity by combining with heavy chains. Together they form the antigen-binding site. The kappa light chain is not part of a multiprotein complex; rather it directly participates in the antibody response. The presence of either kappa or lambda light chains can determine the particularities of antibody characteristics.

Pathways

Kappa light chains are elements of the immunological pathways notably the adaptive immune response. They interact with other proteins like immunoglobulin heavy chains to neutralize pathogens. The constant region of the kappa chain plays a role in determining the isotype of immunoglobulins which affects how antibodies mediate immune effector functions. Cross-linking of surface immunoglobulin by antigens can trigger B cell activation an essential step in the adaptive immune system.

Kappa light chains associate closely with multiple myeloma and certain types of lymphomas. Overproduction of kappa light chains in these diseases results in high levels detectable in the serum or urine which can be used diagnostically as markers. Also dysregulation of kappa chains correlates with autoimmune disorders whereby the immune system incorrectly targets its tissues. In disorders like these the interplay between kappa and lambda chains often arises showing imbalance between the two as a sign of abnormal B cell activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Kappa light chain

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40156854

2025

A hidden intrinsic ability of bicistronic expression based on a novel translation reinitiation mechanism in yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Yiwen Sun,Ralph Bock,Zhichao Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-Kappa light chain antibody [KT102]

Western blot - HRP Anti-Kappa light chain antibody [KT102] (AB170326)

ELISA - HRP Anti-Kappa light chain antibody [KT102] (AB170326)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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