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AB170326

HRP Anti-Kappa light chain antibody [KT102]

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Mouse Monoclonal Kappa light chain antibody - conjugated to HRP. Suitable for ELISA, WB and reacts with Rat samples.

View Alternative Names

HCAK 1, IGKCD, Ig kappa chain C region, Immunoglobulin InV, Immunoglobulin KM, Immunoglobulin kappa constant, Immunoglobulin kappa constant region, Immunoglobulin kappa light chain, Immunoglobulin kappa light chain constant region, Kappa 1 immunoglobulin light chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809, kappa light chain

2 Images
Western blot - HRP Anti-Kappa light chain antibody [KT102] (AB170326)
  • WB

Supplier Data

Western blot - HRP Anti-Kappa light chain antibody [KT102] (AB170326)

All lanes:

Western blot - HRP Anti-Kappa light chain antibody [KT102] (ab170326) at 1/1000 dilution

Lane 1:

rat IgM

Lane 2:

rat IgG2c

Lane 3:

rat IgG2b

Lane 4:

rat IgG2a

Lane 5:

rat IgG1

Predicted band size: 25 kDa

false

ELISA - HRP Anti-Kappa light chain antibody [KT102] (AB170326)
  • ELISA

Supplier Data

ELISA - HRP Anti-Kappa light chain antibody [KT102] (AB170326)

ELISA for crossreactivity to various immunoglobulins.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KT102

Isotype

IgG1

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Rat

Applications

WB, ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "1/2000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>This dilution works using DAB as substrate. More dilution is needed for ECL system.</p>" }, "Mouse": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "1/2000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>This dilution works using DAB as substrate. More dilution is needed for ECL system.</p>" }, "Rat": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/2000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>This dilution works using DAB as substrate. More dilution is needed for ECL system.</p>" }, "Goat": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "1/2000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>This dilution works using DAB as substrate. More dilution is needed for ECL system.</p>" }, "Rabbit": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "1/2000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>This dilution works using DAB as substrate. More dilution is needed for ECL system.</p>" } } }
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We’re constantly upgrading our range to offer the highest quality products. We have selected this alternative product for your review because it gives improved performance, is easier and more convenient to use, or is available faster.

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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab170326 is purified through a Protein A column and labeled with horseradish peroxidase.
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The kappa light chain alternatively known as Ig kappa chain is a component of immunoglobulins which are important for immune response. This protein has a molecular mass of roughly 25 kDa. It predominantly expresses in B lymphocytes where it pairs with heavy chains to form antibodies. Its expression is indicative of mature B cell functions serving as a marker for certain types of immune cells. The kappa light chain can also be detected outside of cells circulating in the blood and other bodily fluids.
Biological function summary

Kappa light chains are integral to the structure and function of antibodies. As part of the immunoglobulin molecule these chains contribute to antigen specificity by combining with heavy chains. Together they form the antigen-binding site. The kappa light chain is not part of a multiprotein complex; rather it directly participates in the antibody response. The presence of either kappa or lambda light chains can determine the particularities of antibody characteristics.

Pathways

Kappa light chains are elements of the immunological pathways notably the adaptive immune response. They interact with other proteins like immunoglobulin heavy chains to neutralize pathogens. The constant region of the kappa chain plays a role in determining the isotype of immunoglobulins which affects how antibodies mediate immune effector functions. Cross-linking of surface immunoglobulin by antigens can trigger B cell activation an essential step in the adaptive immune system.

Kappa light chains associate closely with multiple myeloma and certain types of lymphomas. Overproduction of kappa light chains in these diseases results in high levels detectable in the serum or urine which can be used diagnostically as markers. Also dysregulation of kappa chains correlates with autoimmune disorders whereby the immune system incorrectly targets its tissues. In disorders like these the interplay between kappa and lambda chains often arises showing imbalance between the two as a sign of abnormal B cell activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Kappa light chain
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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