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Rabbit Recombinant Monoclonal KDM1/LSD1 antibody - conjugated to HRP. Nucleus marker. Suitable for IHC-P, WB and reacts with Human, Rat samples.

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Images

Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (AB195897), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (AB195897), expandable thumbnail
  • Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (AB195897), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/5000
Notes

-

Species
Human
Dilution info
1/5000
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

3 products for Alternative Version

Target data

Function

Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context (PubMed:15620353, PubMed:15811342, PubMed:16079794, PubMed:16079795, PubMed:16140033, PubMed:16223729, PubMed:27292636). Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed (PubMed:15620353, PubMed:15811342, PubMed:16079794, PubMed:21300290). Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me (PubMed:15620353, PubMed:20389281, PubMed:21300290, PubMed:23721412). May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity (PubMed:16079794, PubMed:16140033, PubMed:16885027, PubMed:21300290, PubMed:23721412). Also acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in AR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A (PubMed:16079795). Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1 (PubMed:29691401). Demethylates methylated 'Lys-42' and methylated 'Lys-117' of SOX2 (PubMed:29358331). Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (PubMed:16079794, PubMed:16140033). Facilitates epithelial-to-mesenchymal transition by acting as an effector of SNAI1-mediated transcription repression of epithelial markers E-cadherin/CDH1, CDN7 and KRT8 (PubMed:20562920, PubMed:27292636). Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7 (PubMed:20389281).

Alternative names

AOF2, KDM1, KIAA0601, LSD1, KDM1A, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A

Recommended products

Rabbit Recombinant Monoclonal KDM1/LSD1 antibody - conjugated to HRP. Nucleus marker. Suitable for IHC-P, WB and reacts with Human, Rat samples.

Key facts

Isotype
IgG
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6825
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897), expandable thumbnail

    Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)

    ab195897 was shown to specifically react with KDM1 / LSD1 in wild-type HAP1 cells as signal was lost in KDM1A (KDM1 / LSD1) knockout cells. Wild-type and KDM1A (KDM1 / LSD1) knockout samples were subjected to SDS-PAGE. ab195897 and Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

    All lanes: Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897) at 1/5000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: KDM1A (KDM1 / LSD1) knockout HAP1 whole cell lysate at 20 µg

    Predicted band size: 92 kDa

    Observed band size: 110 kDa

    Exposure time: 20min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)

    IHC image of KDM1/LSD1 staining in a section of formalin-fixed paraffin-embedded normal human testis*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab195897 at 1/50 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897), expandable thumbnail

    Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195897 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897) at 1/5000 dilution

    Lane 1: Western blot - HeLa whole cell lysate (HeLa whole cell lysate ab150035) at 10 µg

    Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Lane 3: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 92 kDa

    Observed band size: 110 kDa

    Exposure time: 4min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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