AB208366

HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal L-lactate dehydrogenase B antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, Renal carcinoma antigen NY-REN-46, LDH-H, LDHB

1 Images

Lab

Western blot - HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (AB208366)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208366 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366) at 1/5000 dilution

All lanes:

Western blot - HeLa whole cell lysate (<a href='/en-us/products/cell-lysates/hela-whole-cell-lysate-ab150035'>ab150035</a>) at 10 µg

Predicted band size: 37 kDa

Observed band size: 35 kDa

true

Exposure time: 30s

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y]
Unconjugated

Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y]
Carrier free

Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1565Y

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lactate Dehydrogenase B (LDH-B) also known as LDH is an enzyme that plays an important role in the interconversion of lactate to pyruvate. It belongs to the lactate dehydrogenase family which includes multiple isoenzymes. LDH-B has a molecular weight of approximately 36 kDa and commonly forms a tetramer with three other similar subunits. It is mainly expressed in heart and muscle tissues among others where it functions effectively.

Biological function summary

LDH-B is essential for cellular energy production processes. It catalyzes the conversion of pyruvate a byproduct of glycolysis into lactate while simultaneously converting NADH to NAD+. This reaction is significant under anaerobic conditions where mitochondria-based ATP production is limited. LDH-B does not function alone and can operate as part of the lactate dehydrogenase complex containing different subunit combinations.

Pathways

Lactate and pyruvate metabolism engage LDH-B facilitating the Cori cycle and the lactate shuttle. These pathways enable lactate transport between tissues such as muscle and liver and they are important in maintaining blood glucose levels and energy balance. LDH-B operates alongside its related isoenzymes such as LDH-A which predominantly act in specific tissues and conditions to optimize energy utilization.

Anomalous LDH-B activity associates with conditions like myocardial infarction and some cancer types. Enhanced expression or activity of LDH-B can indicate altered metabolic states in tumors connecting it as a potential marker for certain cancerous conditions. The relationship with other proteins such as the hypoxia-inducible factor (HIF) is notable especially in cancer where HIF can regulate LDH-B expression under low oxygen environments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).

See full target information LDHB

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 9:98 PubMed38609366

2024

Infection with SARS-CoV-2 can cause pancreatic impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Deng,Linlin Bao,Zhiqi Song,Ling Zhang,Pin Yu,Yanfeng Xu,Jue Wang,Wenjie Zhao,Xiuqin Zhang,Yunlin Han,Yanhong Li,Jiangning Liu,Qi Lv,Xujian Liang,Fengdi Li,Feifei Qi,Ran Deng,Siyuan Wang,Yibai Xiong,Ruiping Xiao,Hongyang Wang,Chuan Qin

The international journal of biochemistry & cell b :105935 PubMed33529714

2021

The utilisation of glutamine and glucose by a 3-D tumour model trapped in quiescence.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah Smith,David De Souza,Dedreia Tull,Malcolm McConville,Andrea Pellagatti,Jacqueline Boultwood,Mary Board,Richard Callaghan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y]

Western blot - HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (AB208366)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise