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AB323349

HRP Anti-LI Cadherin antibody [EPR3996]

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Rabbit Recombinant Monoclonal LI Cadherin antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

Cadherin-17, Intestinal peptide-associated transporter HPT-1, Liver-intestine cadherin, LI-cadherin, CDH17

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-LI Cadherin antibody [EPR3996] (AB323349)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-LI Cadherin antibody [EPR3996] (AB323349)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling LI Cadherin with ab323349 at 1/100 (5.0 ug/ml) dilution.

Negative control : no staining on human cerebrum.
The section was incubated with ab323349 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND™ RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-LI Cadherin antibody [EPR3996] (AB323349)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-LI Cadherin antibody [EPR3996] (AB323349)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling LI Cadherin with ab323349 at 1/100 (5.0 ug/ml) dilution.

Membranous staining on human colon.
The section was incubated with ab323349 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND™ RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-LI Cadherin antibody [EPR3996] (AB323349)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-LI Cadherin antibody [EPR3996] (AB323349)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling LI Cadherin with ab323349 at 1/100 (5.0 ug/ml) dilution.

Membranous staining on human colon cancer.
The section was incubated with ab323349 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND™ RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3996

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [SP183] - C-terminal (AB183318)

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Complementary Products

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LI Cadherin also known as Cadherin-17 is a protein that plays a significant role in cell adhesion specifically in epithelial cells. This protein belongs to the cadherin family and has a molecular mass of approximately 88 kDa. LI Cadherin is mainly expressed in the gastrointestinal tract including areas like the stomach and small intestine. It is found on the surfaces of cells where it facilitates cell-cell interaction by binding with other cadherin proteins on adjacent cells.
Biological function summary

This cadherin protein is important for maintaining the integrity of epithelial layers in the gastrointestinal tract. It contributes to the formation of adherens junctions which are necessary for the maintenance of tissue architecture and cellular communication. As part of a larger protein complex in adherens junctions it interacts with cytoskeletal elements to help stabilize cell structures. This mechanical support ensures that cells remain tightly adhered under various physiological conditions.

Pathways

LI Cadherin is involved in pathways that govern cell adhesion and signal transduction. It participates in the Wnt signaling pathway which is vital for cell proliferation and differentiation. Moreover it interacts with E-cadherin and ¦В-catenin within the Wnt pathway aiding in the regulation of gene expression and cellular morphology. These interactions are important for maintaining normal cell behavior and preventing unwanted cell proliferation.

LI Cadherin's expression has been linked to gastrointestinal cancers particularly gastric and colorectal cancers. Aberrations in cadherin-mediated cell adhesion can lead to tumorigenesis as disrupted cell adhesion can cause increased cell motility and invasion. LI Cadherin also engages with proteins such as ¦Б-catenin in tumor cells affecting cellular responses to cancerous transformations. By understanding these relationships researchers aim to develop targeted therapies to address malignancies associated with disrupted cadherin function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.
See full target information Cadherin-17
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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