HRP Anti-Lumican antibody [EPR8898(2)]

• WB

Lab

Western blot - HRP Anti-Lumican antibody [EPR8898(2)] (AB201344)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201344 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Lumican antibody [EPR8898(2)] (ab201344) at 1/5000 dilution

Lane 1:

Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 3:

Y79 (Human retinoblastoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Predicted band size: 38 kDa

Observed band size: 51 kDa

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Exposure time: 2min