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Rabbit Recombinant Monoclonal LXR alpha antibody - conjugated to HRP. Suitable for WB and reacts with Human samples.

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Images

Western blot - HRP Anti-LXR alpha antibody [EPR6508(N)] (AB205874), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Predicted
Rat
Predicted

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

1 product for Alternative Version

Target data

Function

Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed:19481530, PubMed:25661920, PubMed:37478846). Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES (PubMed:37478846). LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides (By similarity). Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8 (PubMed:19481530). Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity). Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis (By similarity). Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators (By similarity).

Alternative names

LXRA, NR1H3, Oxysterols receptor LXR-alpha, Liver X receptor alpha, Nuclear receptor subfamily 1 group H member 3

Recommended products

Rabbit Recombinant Monoclonal LXR alpha antibody - conjugated to HRP. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6508(N)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LXR alpha also known as NR1H3 is a nuclear receptor protein involved in the regulation of lipid metabolism and inflammation. It has a molecular mass of approximately 55 kDa. Expressed mainly in the liver adipose tissue macrophages and intestines LXR alpha acts as a transcription factor controlling the expression of genes important for cholesterol homeostasis. It binds to DNA at specific LXR response elements usually forming heterodimers with the retinoid X receptor (RXR).

Biological function summary

LXR alpha plays a critical role in lipid metabolism and cholesterol efflux. It is part of a larger LXR and CO complex which contributes to the regulation of genes that manage cholesterol absorption transport and excretion. For instance LXR activation increases the expression of ATP-binding cassette transporters such as ABCA1 and ABCG1 which are essential for transporting cholesterol out of cells and facilitating reverse cholesterol transport.

Pathways

The function of LXR alpha is significant in both the cholesterol catabolism pathway and the inflammatory response pathway. Within these pathways it upregulates genes involved in cholesterol catabolism providing a mechanism to prevent excess accumulation in cells and tissues. LXR alpha interacts closely with other nuclear receptors and proteins like PPARs (peroxisome proliferator-activated receptors) highlighting its role in lipid signaling networks.

Associated diseases and disorders

LXR alpha is linked to atherosclerosis and Alzheimer’s disease. Its ability to regulate cholesterol transport and immune function demonstrates involvement in the pathogenesis of atherosclerosis by influencing macrophage-derived foam cell formation. Additionally improper cholesterol metabolism is associated with Alzheimer’s disease where LXR alpha's interaction with amyloid precursor protein (APP) may influence disease progression connecting cholesterol dysfunction with neurodegenerative processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - HRP Anti-LXR alpha antibody [EPR6508(N)] (ab205874), expandable thumbnail

    Western blot - HRP Anti-LXR alpha antibody [EPR6508(N)] (ab205874)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205874 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - HRP Anti-LXR alpha antibody [EPR6508(N)] (ab205874) at 1/5000 dilution

    Lane 1: Liver (Human) Tissue Lysate - fetal normal tissue at 10 µg

    Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Lane 3: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa

    Exposure time: 1min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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