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AB300054

HRP Anti-Maltose Binding Protein antibody [EPR4744]

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Rabbit Recombinant Monoclonal Maltose Binding Protein antibody - conjugated to HRP. Suitable for WB, ELISA and reacts with Escherichia coli samples.

View Alternative Names

b4034, JW3994, malE, Maltose/maltodextrin-binding periplasmic protein, MMBP, Maltodextrin-binding protein, Maltose-binding protein, MBP

2 Images
ELISA - HRP Anti-Maltose Binding Protein antibody [EPR4744] (AB300054)
  • ELISA

Supplier Data

ELISA - HRP Anti-Maltose Binding Protein antibody [EPR4744] (AB300054)

Direct ELISA Dose Response Curve for Maltose Binding Protein using ab300054 :

Antigen : Maltose binding protein at 1000 ng/ml

Primary antibody concentration range : 4000-0 ng/ml

Western blot - HRP Anti-Maltose Binding Protein antibody [EPR4744] (AB300054)
  • WB

Lab

Western blot - HRP Anti-Maltose Binding Protein antibody [EPR4744] (AB300054)

Blocking / Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - HRP Anti-Maltose Binding Protein antibody [EPR4744] (ab300054) at 1/5000 dilution

All lanes:

E.coli lysate

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-Maltose Binding Protein antibody [EPR4744] - BSA and Azide free

  • Unconjugated

    Anti-Maltose Binding Protein antibody [EPR4744]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4744

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Escherichia coli

Applications

WB, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.5 µg/mL", "ELISA-species-notes": "<p>Primary antibody concentration range: 4000-0 ng/ml</p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Maltose Binding Protein often referred to as MBP is a periplasmic protein that plays an important role in the transportation of maltose and maltodextrins in E. coli. It binds to maltose with high specificity facilitating its uptake into the bacterial cell. The MBP protein size is approximately 42 kDa which allows it to effectively interact with its ligands. Scientists often use MBP as a fusion partner in protein purification processes due to its solubility and ability to enhance the expression of recombinant proteins.
Biological function summary

Within the cell it functions by ensuring the effective transport of sugars across the bacterial membrane. Since MBP is not typically a part of a larger complex it acts more as an individual unit facilitating the binding of its target sugars. This binding and transport mechanism is vital for the bacterial carbohydrate metabolism supporting the overall energy balance within the microorganism.

Pathways

This protein is integral to the maltose/maltodextrin transport system which is a part of the larger phosphotransferase system. Its role in these pathways is closely associated with other proteins such as the Maltose ABC transporter proteins which form a transport mechanism across the bacterial inner membrane. Additionally MBP has a functional relationship with the enzymes in the glycolysis pathway as the transported sugars eventually feed into this energy-producing route.

Maltose binding protein itself is not a direct causative factor. However understanding MBP can assist in researching bacterial infections caused by pathogenic strains like E. coli where maltose metabolism is important for survival and virulence. Moreover disruptions in sugar transport pathways involving proteins like MBP can contribute to metabolic imbalances. Although MBP is not directly implicated in human diseases analyzing its function can aid in developing therapeutic approaches targeting sugar transport systems in harmful bacteria.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins such as maltotriose.
See full target information malE
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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