Rabbit Monoclonal Melanoma gp100 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Melanoma gp100 antibody [EP4863(2)] (AB205469), expandable thumbnail](https://content.abcam.com/products/images/hrp-melanoma-gp100-antibody-ep48632-ab205469--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107064.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Melanoma gp100 antibody [EP4863(2)] (AB205469)")
![Western blot - HRP Anti-Melanoma gp100 antibody [EP4863(2)] (AB205469), expandable thumbnail](https://content.abcam.com/products/images/hrp-melanoma-gp100-antibody-ep48632-ab205469--western-blot-img30251.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-Melanoma gp100 antibody [EP4863(2)] (AB205469)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
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6 products for Alternative Version
Target data
Function
Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway. Represents a potent melanoma-specific antigen. Among melanoma non-mutated self-peptides, G9-154 (KTWGQYWQV), G9-209 (ITDQVPFSV) and G9-280 (YLEPGPVTA), appear to act as immunodominant common epitopes that stimulate anti-tumor immune response mediated by HLA-A-restricted cytotoxic T cells.
Alternative names
D12S53E, PMEL17, SILV, PMEL, Melanocyte protein PMEL, ME20-M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, ME20M
Recommended products
Rabbit Monoclonal Melanoma gp100 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP4863(2)
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Melanoma gp100 antibody [EP4863(2)] (ab205469), expandable thumbnail](https://content.abcam.com/products/images/hrp-melanoma-gp100-antibody-ep48632-ab205469--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107064.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Melanoma gp100 antibody [EP4863(2)] (ab205469)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Melanoma gp100 antibody [EP4863(2)] (ab205469)
IHC image of gp100 staining in a section of formalin-fixed paraffin-embedded Human Skin Melanoma*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab205469, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
![Western blot - HRP Anti-Melanoma gp100 antibody [EP4863(2)] (ab205469), expandable thumbnail](https://content.abcam.com/products/images/hrp-melanoma-gp100-antibody-ep48632-ab205469--western-blot-img30251.jpg "Western blot - HRP Anti-Melanoma gp100 antibody [EP4863(2)] (ab205469)")
Western blot - HRP Anti-Melanoma gp100 antibody [EP4863(2)] (ab205469)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205469 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-Melanoma gp100 antibody [EP4863(2)] (ab205469) at 1/1000 dilution
Lane 1: B16-F0 (Mouse) Whole Cell Lysate at 10 µg
Lane 2: Melanoma (Human) Whole Cell Lysate - tumor tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 80 kDa
Exposure time: 8min
Downloads
Product protocols
- Visit the general protocols
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