Rabbit Recombinant Monoclonal Metabotropic Glutamate Receptor 5 antibody - conjugated to HRP. Suitable for IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (AB196482), expandable thumbnail](https://content.abcam.com/products/images/hrp-metabotropic-glutamate-receptor-5-antibody-epr2425y-ab196482--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118300.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (AB196482)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
2 products for Alternative Version
Target data
Function
G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
Alternative names
GPRC1E, MGLUR5, GRM5, Metabotropic glutamate receptor 5, mGluR5
Recommended products
Rabbit Recombinant Monoclonal Metabotropic Glutamate Receptor 5 antibody - conjugated to HRP. Suitable for IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR2425Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab196482), expandable thumbnail](https://content.abcam.com/products/images/hrp-metabotropic-glutamate-receptor-5-antibody-epr2425y-ab196482--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118300.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab196482)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab196482)
IHC image of Metabotropic Glutamate Receptor 5 staining in a section of formalin-fixed paraffin-embedded normal human cerebellum, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196482 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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