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AB199252

HRP Anti-MSI2 antibody [EP1305Y]

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Rabbit Recombinant Monoclonal MSI2 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples.

View Alternative Names

RNA-binding protein Musashi homolog 2, Musashi-2, MSI2

1 Images
Western blot - HRP Anti-MSI2 antibody [EP1305Y] (AB199252)
  • WB

Lab

Western blot - HRP Anti-MSI2 antibody [EP1305Y] (AB199252)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199252 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-MSI2 antibody [EP1305Y] (ab199252) at 1/5000 dilution

All lanes:

SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 20 µg

Predicted band size: 35 kDa

Observed band size: 37 kDa,39 kDa

true

Exposure time: 8min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1305Y

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Based on the immunogen sequence for this antibody, it is not predicted to detect the shorter isoforms of MSI2.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Musashi-2 (MSI2) is a member of the Musashi RNA‐binding protein family also labeled as MSI-2. The protein is approximately 38 kDa in size. It expresses primarily in stem cells and various tumor types including hematopoietic cells and neural tissue. MSI2 plays a role in maintaining stem cell self-renewal and differentiation by regulating mRNA translation through binding to specific target RNAs.
Biological function summary

MSI2 contributes to the regulation of gene expression with a significant impact on cell fate decisions. It acts as part of a post-transcriptional regulatory complex that controls the stability and translation of specific mRNA targets. MSI2 influences pathways involved in stem cell maintenance and differentiation linking it to cellular processes like proliferation and apoptosis. Its activity is important for normal development and the maintenance of undifferentiated cell states.

Pathways

MSI2 has important roles in the Notch and Wnt signaling pathways. These pathways are vital for stem cell maintenance and tissue regeneration. MSI2 regulates components of these pathways interacting with proteins such as beta-catenin in Wnt signaling. This regulation enhances its involvement in controlling cell proliferation and differentiation contributing to signaling processes that determine cellular behavior.

MSI2 is linked to acute myeloid leukemia (AML) and certain types of solid tumors. Overexpression of MSI2 often correlates with poor prognosis in AML where it impacts cancer progression by modulating genes associated with cell growth. Additionally its interaction with oncogenic proteins like BCR-ABL suggests its involvement in cancerogenesis through disease-linked pathways. Research indicates MSI2 as a potential target for therapeutic intervention in such malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
See full target information MSI2
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Product promise

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For full details, please see our Terms & Conditions

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