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AB195936

HRP Anti-MUC1 antibody [EPR1023]

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(2 Publications)

Rabbit Recombinant Monoclonal MUC1 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

CD227, PUM, MUC1, Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, CA 15-3, KL-6, PEM, EMA

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-MUC1 antibody [EPR1023] (AB195936)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-MUC1 antibody [EPR1023] (AB195936)

IHC image of MUC1 staining in a section of formalin-fixed paraffin-embedded normal human kidney*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195936, 1/70 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1023

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
See full target information MUC1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 34:436-44 PubMed24469049

2014

TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis.

Applications

Unspecified application

Species

Human

A R Cyr,M V Kulak,J M Park,M V Bogachek,P M Spanheimer,G W Woodfield,L S White-Baer,Y Q O'Malley,S L Sugg,A K Olivier,W Zhang,F E Domann,R J Weigel

Gastroenterology 145:1110-20 PubMed23896173

2013

A gene expression signature of epithelial tubulogenesis and a role for ASPM in pancreatic tumor progression.

Applications

WB

Species

Human

Wei-Yu Wang,Chung-Chi Hsu,Ting-Yun Wang,Chi-Rong Li,Ya-Chin Hou,Jui-Mei Chu,Chung-Ta Lee,Ming-Sheng Liu,Jimmy J-M Su,Kuan-Ying Jian,Shenq-Shyang Huang,Shih-Sheng Jiang,Yan-Shen Shan,Pin-Wen Lin,Yin-Ying Shen,Michael T-L Lee,Tze-Sian Chan,Chun-Chao Chang,Chung-Hsing Chen,I-Shou Chang,Yen-Ling Lee,Li-Tzong Chen,Kelvin K Tsai
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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