AB215778

HRP Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]

BOND RX™ Validated
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(1 Publication)

Mouse Monoclonal MUC1 antibody - conjugated to HRP. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD227, PUM, MUC1, Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, CA 15-3, KL-6, PEM, EMA

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB215778)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB215778)

IHC image of MUC1 staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab215778, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Carrier free

Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] - BSA and Azide free
Unconjugated

Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]
578 PE

PE Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HMFG1 (aka 1.10.F3)

Isotype

IgG1

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human

Applications

IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }

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Product details

For a more comprehensive guide to this epitope of HMFG1 clone, we recommend the following publications;

Petrakou E et al. 1998: Epitope Mapping of Anti-MUC1 Mucin Protein Core Monoclonal Antibodies. Tumour Biology.PMID: 9422085

Verhoeyen ME et al. 1993: Construction of a reshaped HMFG1 antibody and comparison of its fine specificity with that of the parent mouse antibody. Immunology. PMID: 7682986

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

See full target information MUC1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 23:145 PubMed35350592

2022

Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolin Yang,Yukun Li,Geling Liu,Weina Zha,Ying Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB215778)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise