JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB218713

HRP Anti-Mucin 5AC antibody [EPR16904]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal Mucin 5AC antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MUC5, MUC5AC, Mucin-5AC, MUC-5AC, Gastric mucin, Major airway glycoprotein, Tracheobronchial mucin, TBM

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Mucin 5AC antibody [EPR16904] (AB218713)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Mucin 5AC antibody [EPR16904] (AB218713)

IHC image of Mucin 5AC staining in a section of formalin-fixed paraffin-embedded normal human stomach*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab218713, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16904

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB218466

Anti-Mucin 5AC antibody [MUC5AC/917]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [MUC5AC/917] (AB218466)

  • 0
  • 0
Primary Antibodies

AB216007

Anti-Mucin 5AC antibody [MUC5AC/917] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [MUC5AC/917] - BSA and Azide free (AB216007)

  • 0
  • 0
Primary Antibodies

AB24070

Anti-Mucin 5AC antibody [1-13M1]

Flow Cytometry - Anti-Mucin 5AC antibody [1-13M1] (AB24070)

  • 0
  • 0
Primary Antibodies

AB24071

Anti-Mucin 5AC antibody [2-11M1]

Flow Cytometry - Anti-Mucin 5AC antibody [2-11M1] (AB24071)

  • 0
  • 0
Primary Antibodies

AB3649

Anti-Mucin 5AC antibody [45M1]

BOND RX™ Validated|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (AB3649)

  • 5
  • 8
Primary Antibodies

AB212636

Anti-Mucin 5AC antibody [45M1] - BSA and Azide free

BOND RX™ Validated|Advanced Validation|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (AB212636)

  • 5
  • 4
Primary Antibodies

AB77576

Anti-Mucin 5AC antibody [CLH2]

Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [CLH2] (AB77576)

  • 0
  • 1

Complementary Products

Assay Kits

AB303761

Human Mucin 5AC ELISA Kit - Extracellular

Recombinant|SimpleStep

ELISA - Human Mucin 5AC ELISA Kit - Extracellular (AB303761)

  • 5
  • 1
Primary Antibodies

AB193895

Alexa Fluor® 647 Anti-Cytokeratin 5 antibody [EP1601Y]

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Cytokeratin 5 antibody [EP1601Y] (AB193895)

  • 5
  • 4
Primary Antibodies

AB204034

Alexa Fluor® 647 Anti-beta IV Tubulin antibody [EPR16775]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-beta IV Tubulin antibody [EPR16775] (AB204034)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB218591

Alexa Fluor® 647 Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB218591)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mucin 5AC also known simply as MUC5AC is a high molecular weight glycoprotein with a mass in the megadaltons typically found in the human respiratory gastrointestinal and reproductive tracts. It is a major component of mucus which plays a critical role in protecting and lubricating the epithelial surfaces of these organs. MUC5AC is primarily expressed in the goblet cells of the respiratory epithelium and the stomach lining. Due to its significance researchers often use a MUC5AC ELISA or other detection methods such as tagging with Alexa Fluor 555 to measure its levels in various tissues.
Biological function summary

The functions of MUC5AC are central to the maintenance of the protective mucus barrier on epithelial surfaces preventing pathogen invasion and facilitating the clearance of inhaled particles. It is a part of the gel-forming mucin family and often forms complex structures by polymerizing with other mucins like MUC2 and MUC5B contributing to the viscoelastic properties of mucus. These interactions ensure the formation of a stable and robust mucosal barrier.

Pathways

The regulation of MUC5AC production involves several signaling pathways including the EGFR (epidermal growth factor receptor) pathway and the IL-13/STAT6 pathway. These pathways influence mucin production and secretion particularly in response to growth factors and inflammatory cytokines. Proteins such as 13M1 and 11M1 interact with MUC5AC within these pathways modulating its expression and function. This regulation is essential for normal mucus homeostasis and response to environmental challenges.

MUC5AC has a strong association with respiratory conditions like asthma and chronic obstructive pulmonary disease (COPD). In these diseases the overproduction or altered composition of MUC5AC contributes to airway obstruction and reduced pulmonary function. MUC5AC levels can be indicative of disease severity and are often measured via tests like the 5AC blood test. In these contexts proteins such as 5AC protein and 45M1 are often studied for their roles in facilitating disease progression and as potential therapeutic targets to modulate mucin levels in affected individuals.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed : 14535999, PubMed : 14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed : 14535999).
See full target information MUC5AC
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1598436 PubMed40621452

2025

Tumor-suppressing multi-enterobacteria and PD-1/PD-L1 immune checkpoint inhibitor combination improves the outcome of hepatocellular carcinoma therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Dongjuan Wu,Yaohua Fan,Mingsheng Zhang,Xiaoguang Wang,Xuesong He,Xinwei Guo,Yangchen Liu,Chenxi Cao,Zhaoqun Deng
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com