AB185058

HRP Anti-muscle Actin antibody [EPR8484] - Loading Control

Name: HRP Anti-muscle Actin antibody [EPR8484] (ab185058) | Abcam
Brand: Abcam Limited
SKU: AB185058
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(2 Publications)

Rabbit Recombinant Monoclonal alpha skeletal muscle Actin antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

ACTA, ACTA1, Alpha-actin-1

1 Images

Lab

Western blot - HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (AB185058)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab185058 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406

All lanes:

Western blot - HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058) at 1/10000 dilution

Lane 1:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Lane 2:

Human kidney tissue lysate - total protein (<a href='/en-us/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 20 µg

Lane 3:

Human Fetal Heart at 5 µg

Lane 4:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Lane 5:

Skeletal Muscle (Rat) Tissue Lysate at 10 µg

Predicted band size: 42 kDa

Observed band size: 42 kDa

true

Exposure time: 5s

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-muscle Actin antibody [EPR8484] - Cytoskeleton Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-muscle Actin antibody [EPR8484] - Cytoskeleton Marker
Unconjugated

Anti-muscle Actin antibody [EPR8484] - Loading Control
Carrier free

Anti-muscle Actin antibody [EPR8484] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8484

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Mouse, Rat, Human, Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Muscle Actin also known as ACTA1 is a protein that plays a significant role in muscle contraction. It has a molecular weight of approximately 42 kDa. This protein is primarily found in skeletal muscle tissues. Muscle Actin exists in two forms: G-actin a globular monomer and F-actin a filamentous polymer that forms actin filaments. These filaments provide structural support and are involved in muscle contraction through their interaction with myosin.

Biological function summary

Muscle Actin interacts with myosin to facilitate muscle contraction. It is an important component of the thin filament within the sarcomere the basic unit of muscle fiber. Muscle Actin is part of a complex and this complex includes other important proteins like tropomyosin and troponin. These proteins regulate the interaction between actin and myosin enabling the muscle contraction process. Muscle Actin's polymerization and depolymerization dynamics are important for the regulation of cellular movement and structural integrity.

Pathways

Muscle Actin is essential for the actin-myosin contractile machinery in muscle cells which is part of the sarcomere assembly pathway. This process involves other proteins such as myosin heavy chain and tropomyosin which are important for muscle contraction. Another important pathway is the Rho GTPase signaling pathway which controls actin filament assembly and is involved in a variety of cellular processes including muscle contraction and cell motility.

Muscle Actin mutations link to certain muscle-related diseases such as nemaline myopathy and congenital myopathies. Nemaline myopathy involves the presence of rod-like structures in muscle fibers and it results from anomalies in actin filaments. Muscle Actin also associates with cardiac muscle conditions when there are mutations affecting cardiac actin structure or function. This reveals the connection between Muscle Actin tropomyosin and troponin in muscle contraction efficiency and structural integrity contributing to these diseases' pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Artificial organs 44:E406-E418 PubMed32279354

2020

Cold-inducible RNA binding protein agonist enhances the cardioprotective effect of UW solution during extended heart preservation.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Zhou Pan,Li-Jing Zhang,Yi-Wei Liu,Yong-Nan Li,Zhan-Hao Su,Jian Meng,Hao Zhang

Nature 524:114-8 PubMed26200337

2015

CDA directs metabolism of epigenetic nucleosides revealing a therapeutic window in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Melania Zauri,Georgina Berridge,Marie-Laëtitia Thézénas,Kathryn M Pugh,Robert Goldin,Benedikt M Kessler,Skirmantas Kriaucionis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-muscle Actin antibody [EPR8484] - Loading Control

Western blot - HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (AB185058)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise