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AB62928

HRP Anti-Myc tag antibody [9E10]

5

(2 Reviews)

|

(37 Publications)

Anti-Myc tag antibody [9E10] - HRP conjugated (ab62928) is a mouse monoclonal antibody detecting Myc tag in Western Blot, ELISA.

- Over 30 publications
- Trusted since 2008

View Alternative Names

Myc Epitope Tag, c-myc tag

2 Images
Western blot - HRP Anti-Myc tag antibody [9E10] (AB62928)
  • WB

Ap

Western blot - HRP Anti-Myc tag antibody [9E10] (AB62928)

All lanes:

Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928) at 1/10000 dilution

All lanes:

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (<a href='/en-us/products/cell-lysates/e-coli-positive-control-escherichia-coli-whole-cell-lysate-ab5395'>ab5395</a>) at 0.1 µg

Predicted band size: 49 kDa

Observed band size: 41 kDa

false

Western blot - HRP Anti-Myc tag antibody [9E10] (AB62928)
  • WB

Lab

Western blot - HRP Anti-Myc tag antibody [9E10] (AB62928)

Blocking buffer : 2% BSA Gel type : MOPS

All lanes:

Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928) at 1/10000 dilution

All lanes:

Western blot - Recombinant E. coli Multi Tag protein (<a href='/en-us/products/proteins-peptides/recombinant-e-coli-multi-tag-protein-ab36791'>ab36791</a>) at 0.5 µg

Predicted band size: 49 kDa

Observed band size: 45 kDa

false

Exposure time: 4min

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Myc tag antibody [9E10]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Myc tag antibody [9E10]

  • 660 APC

    APC Anti-Myc tag antibody [9E10]

  • 578 PE

    PE Anti-Myc tag antibody [9E10]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Myc tag antibody [9E10]

  • Biotin

    Biotin Anti-Myc tag antibody [9E10] - C-terminal

  • 519 FITC

    FITC Anti-Myc tag antibody [9E10]

  • Biotin

    Biotin Anti-Myc tag antibody [9E10]

  • 657 SureLight® APC

    SureLight® APC Anti-Myc tag antibody [9E10]

  • 576 DyLight® 550

    DyLight® 550 Anti-Myc tag antibody [9E10]

  • 673 DyLight® 650

    DyLight® 650 Anti-Myc tag antibody [9E10]

  • 518 DyLight® 488

    DyLight® 488 Anti-Myc tag antibody [9E10]

  • Unconjugated

    Anti-Myc tag antibody [9E10]

  • Carrier free

    Anti-Myc tag antibody [9E10] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9E10

Isotype

IgG1

Light chain type

kappa

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

WB, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/100 - 1/500", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" } } }

Product details

What is this antibody validated in?
HRP Anti-Myc tag antibody [9E10] (ab62928) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), ELISA. Tagged protein detection
Myc tag antibodies are used to visualize proteins labelled with this tag in a variety of applications.

What is the molecular weight of Myc tag?
Anti-Myc tag [9E10] HRP (ab62928) specifically detects a band for Myc tag (UniProt: P01106) at a molecular weight of 49kDa.

Trusted by the scientific community
Anti-Myc tag [9E10] HRP (ab62928) was first used in a scientific publication in 2008 and has been cited over 30 times in peer-reviewed journals.

Related products
Antibody clone 9E10 is also available pre-conjugated to a variety of labels for your convenience – Anti-Myc tag HRP [9E10] (ab62928).

Other related products
We have a range of other formats of antibody clone [9E10] also available for your convenience: ab185655, Carrier free - ab236021, PE - ab237249, Alexa Fluor® 488 - ab300546, Alexa Fluor® 647 - ab300731, APC - ab310838, Alexa Fluor® 594 - ab311655, Alexa Fluor® 568 - ab312928, Alexa Fluor® 555 - ab313141, HRP - ab62928, PE - ab72468, SureLight® APC - ab72580, Biotin - ab81658, DyLight® 650 - ab117487, Carrier free - ab117499, FITC - ab117599

Properties and storage information

Form
Liquid
Purity
IgG fraction
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Store in the dark

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Journal of medicinal chemistry 68:8484-8496 PubMed40208951

2025

Development of Galectin-7-Specific Nanobodies: Implications for Immunotherapy and Molecular Imaging in Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rita Nehmé,Marlène Fortier,Myriam Létourneau,Camille Fuselier,Philippine Granger Joly de Boissel,Alyssa Dumoulin,Brigitte Guérin,Véronique Dumulon-Perreault,Samia Ait-Mohand,Otman Sarrhini,Sacha T Larda,Yarileny Castellanos Villamizar,Mighel Bernier,Natalia Porębska,Łukasz Opaliński,David Chatenet,Nicolas Doucet,Yves St-Pierre

Nature communications 16:676 PubMed39809784

2025

CPK1 activates CNGCs through phosphorylation for Ca signaling to promote root hair growth in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Meijun Zhu,Bo-Ya Du,Yan-Qiu Tan,Yang Yang,Yang Zhang,Yong-Fei Wang

Nature communications 15:7984 PubMed39266569

2024

Nuclear lamin A/C phosphorylation by loss of androgen receptor leads to cancer-associated fibroblast activation.

Applications

Unspecified application

Species

Unspecified reactive species

Soumitra Ghosh,Jovan Isma,Paola Ostano,Luigi Mazzeo,Annagiada Toniolo,Monalisa Das,Joni R White,Christian Simon,G Paolo Dotto

iScience 27:110567 PubMed39184446

2024

A high-throughput screening identifies MCM chromatin loading inhibitors targeting cells with increased replication origins.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Falbo,Hervé Técher,Vincenzo Sannino,Michela Robusto,Giovanni Fagà,Federica Pezzimenti,Francesco Romeo,Luca Gabriele Colombo,Stefania Vultaggio,Daniele Fancelli,Silvia Monzani,Valentina Cecatiello,Sebastiano Pasqualato,Mario Varasi,Ciro Mercurio,Vincenzo Costanzo

Cancer discovery 14:2471-2488 PubMed39058094

2024

MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Olaf Klingbeil,Damianos Skopelitis,Claudia Tonelli,Toyoki Yoshimoto,Aktan Alpsoy,Maria C Panepinto,Francesca Minicozzi,Joseph R Merrill,Amanda M Cafiero,Disha Aggarwal,Suzanne Russo,Taehoon Ha,Osama E Demerdash,Tse-Luen Wee,David L Spector,Scott K Lyons,David A Tuveson,Paolo Cifani,Christopher R Vakoc

Cell reports 43:114510 PubMed39018246

2024

Proteomic analysis reveals a PLK1-dependent G2/M degradation program and a role for AKAP2 in coordinating the mitotic cytoskeleton.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan D Mouery,Kimberly Lukasik,Carolyn Hsu,Thomas Bonacci,Derek L Bolhuis,Xianxi Wang,C Allie Mills,E Drew Toomer,Owen G Canterbury,Kevin C Robertson,Timothy B Branigan,Nicholas G Brown,Laura E Herring,Stephanie L Gupton,Michael J Emanuele

Journal of virology 97:e0020023 PubMed36971578

2023

A Novel Vpu Adaptive Mutation of HIV-1 Degrades Tetherin in Northern Pig-Tailed Macaques (Macaca leonina) Mainly via the Ubiquitin-Proteasome Pathway and Increases Viral Release.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Lu,Wei Pang,Man-Di Zhang,Jia-Hao Song,Fan Shen,Wen-Qiang He,Yong-Tang Zheng

Oncogene 41:4537-4546 PubMed36064578

2022

Targeted demethylation at ZNF154 promotor upregulates ZNF154 expression and inhibits the proliferation and migration of Esophageal Squamous Carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting He,Shiyong Lin,Yi Guo,Yuanzhong Wu,Lu-Lu Zhang,Qiling Deng,Zi-Ming Du,Mingbiao Wei,Weijie Zhu,Wan-Juan Chen,Jian-Yong Shao,Guo-Liang Xu

EMBO reports 23:e53608 PubMed35437868

2022

XIAP promotes melanoma growth by inducing tumour neutrophil infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Mila Daoud,Pia Nora Broxtermann,Fabian Schorn,J Paul Werthenbach,Jens Michael Seeger,Lars M Schiffmann,Kerstin Brinkmann,Domagoj Vucic,Thomas Tüting,Cornelia Mauch,Dagmar Kulms,Paola Zigrino,Hamid Kashkar

Nature communications 12:1222 PubMed33619272

2021

Checkpoint inhibition through small molecule-induced internalization of programmed death-ligand 1.

Applications

Unspecified application

Species

Unspecified reactive species

Jang-June Park,Emily P Thi,Victor H Carpio,Yingzhi Bi,Andrew G Cole,Bruce D Dorsey,Kristi Fan,Troy Harasym,Christina L Iott,Salam Kadhim,Jin Hyang Kim,Amy C H Lee,Duyan Nguyen,Bhavna S Paratala,Ruiqing Qiu,Andre White,Damodharan Lakshminarasimhan,Christopher Leo,Robert K Suto,Rene Rijnbrand,Sunny Tang,Michael J Sofia,Chris B Moore
View all publications

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