AB77895

HRP Anti-Myelin Basic Protein antibody

Name: HRP Anti-Myelin Basic Protein antibody (ab77895) | Abcam
Brand: Abcam Limited
SKU: AB77895
Price: 535 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(4 Publications)

Rabbit Polyclonal Myelin Basic Protein antibody - conjugated to HRP. Suitable for sELISA and reacts with Pig, Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow MBP.

View Alternative Names

Myelin basic protein, MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein

3 Images

Sandwich ELISA - HRP Anti-Myelin Basic Protein antibody (AB77895)

sELISA

Unknown

Sandwich ELISA - HRP Anti-Myelin Basic Protein antibody (AB77895)

Sandwich ELISA detection of mouse Myelin Basic Protein in the plasma with un-optimized sensitivity 0.1 ng/mL.

sELISA

Unknown

Sandwich ELISA - HRP Anti-Myelin Basic Protein antibody (AB77895)

Immobilized Anti-MBP (ab28541) : range from 0.125 to 2 μg/ml, 100 μl/well
Fixed Captured Antigen : Swine MBP, 500 ng/mL
Detection : anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt

sELISA

Unknown

Sandwich ELISA - HRP Anti-Myelin Basic Protein antibody (AB77895)

Fixed Immobilized Anti-MBP (ab28541) : 2 ug/mL, 100 ul/well
Captured Antigen : Swine MBP , range from 1 to 90 ng/mL
Detection : anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Mouse, Human, Pig

Applications

sELISA

applications

Immunogen

Native Full Length Protein corresponding to Cow MBP.

P02687

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "1/10000", "sELISA-species-notes": "To be used as detection antibody. Pair with <a href='/en-us/products/primary-antibodies/myelin-basic-protein-antibody-ab28541'>ab28541</a> - Rabbit polyclonal to Myelin Basic Protein" }, "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "1/10000", "sELISA-species-notes": "To be used as detection antibody. Pair with <a href='/en-us/products/primary-antibodies/myelin-basic-protein-antibody-ab28541'>ab28541</a> - Rabbit polyclonal to Myelin Basic Protein" }, "Pig": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "1/10000", "sELISA-species-notes": "To be used as detection antibody. Pair with <a href='/en-us/products/primary-antibodies/myelin-basic-protein-antibody-ab28541'>ab28541</a> - Rabbit polyclonal to Myelin Basic Protein" } } }

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Product details

The purified antibody is conjugated to peroxidase (HRP) through reductive amination. Reductive amination with Ab/HRP ratio: 1/20.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6 - 8 Constituents: Tris buffered saline, 5% BSA, 2.94% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The myelin basic protein also referred to as MBP or MBP protein plays an important mechanical role in the central nervous system. It is a major component in the formation and stability of the myelin sheath which wraps around nerve fibers to ensure efficient neural signaling. The molecular weight of MBP can vary due to alternative splicing; one common form has a mass of about 18.5 kDa. MBP is expressed predominantly in oligodendrocytes within the brain and spinal cord where it helps form the myelin sheath. MBP2 is another variant that may be mentioned in some contexts.

Biological function summary

MBP is not just a passive structural element; it binds and interacts with lipids and other proteins to maintain the compact structure of the myelin sheath. MBP forms part of a complex that includes various other proteins and lipids ensuring the stability and function of myelin. Misfolding or altered expression of MBP can compromise myelin integrity affecting the insulation of neural pathways and signal transmission.

Pathways

MBP is an essential component in the pathways of myelination and neural plasticity. It plays a role in the signaling pathways that stimulate the development and maintenance of the myelin sheath. The protein interacts with other elements of the myelin sheath such as proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) to coordinate its formation and repair processes.

MBP is linked closely to multiple sclerosis (MS) and other demyelinating conditions. Damage to or autoimmunity against MBP leads to the degradation of the myelin sheath contributing to the neurological symptoms in MS. The protein interacts with elements of the immune system with autoantibodies targeting MBP often found in MS patients. Another disorder associated with MBP dysfunction is Charcot-Marie-Tooth disease where abnormal MBP expression and structure can impact nerve function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

See full target information MBP

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cytokine 113:417-426 PubMed30539784

2018

The protective effect of rifampicin on behavioral deficits, biochemical, and neuropathological changes in a cuprizone model of demyelination.

Applications

Unspecified application

Species

Unspecified reactive species

Hamid Zahednasab,Masoumeh Firouzi,Sussan Kaboudanian-Ardestani,Zahra Mojallal-Tabatabaei,Sajad Karampour,Hossein Keyvani

International journal of stroke : official journal of the International Stroke Society 14:310-320 PubMed30196792

2018

A protocol for precise comparisons of small vessel disease lesions between ex vivo magnetic resonance imaging and histopathology.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine A Humphreys,Maurits A Jansen,Susana Muñoz Maniega,Víctor González-Castro,Cyril Pernet,Ian J Deary,Rustam Al-Shahi Salman,Joanna M Wardlaw,Colin Smith

BioMed research international 2014:926394 PubMed25276831

2014

Glatiramer acetate and nanny proteins restrict access of the multiple sclerosis autoantigen myelin basic protein to the 26S proteasome.

Applications

Species

Cow

Ekaterina Kuzina,Anna Kudriaeva,Ivan Smirnov,Michael V Dubina,Alexander Gabibov,Alexey Belogurov

Annals of the rheumatic diseases 70:188-93 PubMed20736390

2010

Anti-cyclic citrullinated peptide antibodies are a collection of anti-citrullinated protein antibodies and contain overlapping and non-overlapping reactivities.

Applications

Species

Human

Andreea Ioan-Facsinay,Hanane el-Bannoudi,Hans U Scherer,Diane van der Woude,Henri A Ménard,Maximilien Lora,Leendert A Trouw,Tom W J Huizinga,Rene E M Toes

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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HRP Anti-Myelin Basic Protein antibody

Sandwich ELISA - HRP Anti-Myelin Basic Protein antibody (AB77895)

Sandwich ELISA - HRP Anti-Myelin Basic Protein antibody (AB77895)

Sandwich ELISA - HRP Anti-Myelin Basic Protein antibody (AB77895)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise