AB199243

HRP Anti-Myoglobin antibody [EP3081Y]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Myoglobin antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

Myoglobin, Nitrite reductase MB, Pseudoperoxidase MB, MB

1 Images

Lab

Western blot - HRP Anti-Myoglobin antibody [EP3081Y] (AB199243)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199243 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Myoglobin antibody [EP3081Y] (ab199243) at 1/5000 dilution

Lane 1:

Heart (Human) Tissue Lysate - adult normal tissue at 10 µg

Lane 2:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 3:

Heart (Rat) Tissue Lysate at 10 µg

Predicted band size: 17 kDa

Observed band size: 17 kDa

true

Exposure time: 2s

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Myoglobin antibody [EP3081Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Myoglobin antibody [EP3081Y]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Myoglobin antibody [EP3081Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Myoglobin antibody [EP3081Y]
578 PE

PE Anti-Myoglobin antibody [EP3081Y]
660 APC

APC Anti-Myoglobin antibody [EP3081Y]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Myoglobin antibody [EP3081Y]
Unconjugated

Anti-Myoglobin antibody [EP3081Y]
Carrier free

Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP3081Y

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Mouse, Rat, Human, Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myoglobin also known as MB is a small globular protein with a molecular weight of approximately 17 kDa. It functions as an oxygen-binding protein and is expressed mainly in cardiac and skeletal muscle tissue where it facilitates oxygen storage and transport. The myoglobin protein plays an important role in maintaining the oxygen supply needed during muscular contraction and intense physical activity.

Biological function summary

Myoglobin in muscle cells acts to store oxygen which provides a rapid release when required during muscle contraction. Myoglobin serves as a monomer and does not form part of a complex. Its structure allows it to temporarily store and relay oxygen where it is most required enhancing the often abrupt demands of muscles for oxygen. The ability of myoglobin to bind oxygen and release it under hypoxic conditions is central to its biological role in vertebrates.

Pathways

The function of myoglobin in aerobic respiration in muscles involves its participation in the oxygen transport pathway. This protein closely interacts with hemoglobin to mobilize oxygen effectively to mitochondria during muscle contraction. Unlike hemoglobin myoglobin has a hyperbolic oxygen dissociation curve which allows it to provide oxygen at lower partial pressures contributing significantly to the efficient metabolism during hypoxia or intense muscular exertion.

Myoglobin plays a significant role in conditions such as rhabdomyolysis and myocardial infarction. Rhabdomyolysis a syndrome caused by muscle injury results in the release of myoglobin into the bloodstream. Myoglobin detection kits including myoglobin ELISA are essential tools for the diagnosis of these conditions. Moreover its rapid increase in plasma levels after heart muscle damage enables its use as an early marker for myocardial infarction. Myoglobin's interaction with proteins like creatine kinase provides valuable information on muscle damage and cardiac events.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand (PubMed : 30918256, PubMed : 34679218). Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide (PubMed : 32891753). Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity (PubMed : 34679218).

See full target information MB

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:4491 PubMed40368919

2025

Inherited deficiency of DIAPH1 identifies a DNA double strand break repair pathway regulated by γ-actin.

Applications

Unspecified application

Species

Unspecified reactive species

Beth L Woodward,Sudipta Lahiri,Anoop S Chauhan,Marcos Rios Garcia,Lucy E Goodley,Thomas L Clarke,Mohinder Pal,Angelo Agathanggelou,Satpal S Jhujh,Anil N Ganesh,Fay M Hollins,Valentina Galassi Deforie,Reza Maroofian,Stephanie Efthymiou,Andrea Meinhardt,Christopher G Mathew,Michael A Simpson,Heather C Mefford,Eissa A Faqeih,Sergio D Rosenzweig,Stefano Volpi,Gigliola Di Matteo,Caterina Cancrini,Annarita Scardamaglia,Fiona Shackley,E Graham Davies,Shahnaz Ibrahim,Peter D Arkwright,Maha S Zaki,Tatjana Stankovic,A Malcolm R Taylor,Antonina J Mazur,Nataliya Di Donato,Henry Houlden,Eli Rothenberg,Grant S Stewart

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-Myoglobin antibody [EP3081Y]

Western blot - HRP Anti-Myoglobin antibody [EP3081Y] (AB199243)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise