Mouse Recombinant Monoclonal Fast Myosin Skeletal Heavy chain antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Mouse, Rat samples.
Images
![Western blot - HRP Anti-Myosin antibody [A4.1025] (AB323782), expandable thumbnail](https://content.abcam.com/products/images/hrp-myosin-antibody-a41025-ab323782--western-blot-img446050.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-Myosin antibody [A4.1025] (AB323782)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Myosin antibody [A4.1025] (AB323782), expandable thumbnail](https://content.abcam.com/products/images/hrp-myosin-antibody-a41025-ab323782--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446060.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Myosin antibody [A4.1025] (AB323782)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Myosin antibody [A4.1025] (AB323782), expandable thumbnail](https://content.abcam.com/products/images/hrp-myosin-antibody-a41025-ab323782--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446059.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Myosin antibody [A4.1025] (AB323782)")
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 | Notes - |
Species Rat | Dilution info 1/5000 | Notes - |
Alternative names
Myosin-1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d, MyHC-2x, MyHC-IIx/d, MYH1
Mouse Recombinant Monoclonal Fast Myosin Skeletal Heavy chain antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- A4.1025
- Purification technique
- Affinity purification Protein A
- Specificity
ATP binding loop of myosin heavy chain skeletal and cardiac muscle.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myosin is a molecular motor protein responsible for converting chemical energy into mechanical force facilitating muscle contraction and cellular movement. Also known as the myosin heavy chain (MHC) or MYH myosin has a commonly referenced heavy chain Myosin heavy chain 1 (MYH1) with a molecular mass of roughly 200 kDa. Myosin is broadly expressed in muscle tissues including skeletal cardiac and smooth muscles and appears in various isoforms depending on the specific type of muscle tissue.
- Biological function summary
Myosin interacts intricately with actin filaments to form a complex that executes muscle contraction and cell motility. Myosin heads bind to actin filaments employing ATP hydrolysis to generate motion. This protein is a fundamental component of the sarcomere the structural unit of muscle fibers and works in concert with other proteins like tropomyosin and troponin to regulate muscle contraction.
- Pathways
Myosin has a significant role in the muscle contraction and cell motility pathways. Myosin and actin interaction is central to the sliding filament theory which is an essential part of the muscle contraction pathway. Myosin is also involved in non-muscle cell movement through the actin-myosin contractile ring in the process of cytokinesis. Myosin interacts with proteins such as actin and ATP in these pathways to facilitate cellular processes.
- Associated diseases and disorders
Myosin has connections to conditions like hypertrophic cardiomyopathy and various myopathies. Mutations in the myosin heavy chain can lead to hypertrophic cardiomyopathy affecting the heart's ability to pump blood effectively. Additionally disruptions in myosin function can contribute to muscular diseases impairing muscle strength and function. Myosin's interaction with actin and related sarcomeric proteins is pivotal in these pathological scenarios often resulting in altered muscle dynamics and associated clinical manifestations.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - HRP Anti-Myosin antibody [A4.1025] (ab323782), expandable thumbnail](https://content.abcam.com/products/images/hrp-myosin-antibody-a41025-ab323782--western-blot-img446050.jpg "Western blot - HRP Anti-Myosin antibody [A4.1025] (ab323782)")
Western blot - HRP Anti-Myosin antibody [A4.1025] (ab323782)
Myosin Western blot staining using mouse Anti-Myosin antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - HRP Anti-Myosin antibody [A4.1025] (ab323782) at 1/5000 dilution
Lane 1: Mouse skeletal musle tissue lysate at 20 µg
Lane 2: Rat skeletal muscle tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 20 µg
Lane 4: Rat liver tissue lysate at 20 µg
Observed band size: 250 kDa, 124 kDa
Exposure time: 1s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Myosin antibody [A4.1025] (ab323782), expandable thumbnail](https://content.abcam.com/products/images/hrp-myosin-antibody-a41025-ab323782--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446060.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Myosin antibody [A4.1025] (ab323782)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Myosin antibody [A4.1025] (ab323782)
Myosin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat cardiac muscle using mouse Anti-Myosin antibody
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Myosin with ab323782 at 1/1000 (0.5 ug/ml) dilution.
Positive staining on rat cardiac muscle.
The section was incubated with ab323782 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Myosin antibody [A4.1025] (ab323782), expandable thumbnail](https://content.abcam.com/products/images/hrp-myosin-antibody-a41025-ab323782--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446059.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Myosin antibody [A4.1025] (ab323782)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Myosin antibody [A4.1025] (ab323782)
Myosin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse cardiac muscle using mouse Anti-Myosin antibody
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Myosin with ab323782 at 1/1000 (0.5 ug/ml) dilution.
Positive staining on mouse cardiac muscle.
The section was incubated with ab323782 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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