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AB209040

HRP Anti-NDUFV2 antibody [EPR15351(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal NDUFV2 antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NDUFV2, NADH-ubiquinone oxidoreductase 24 kDa subunit

1 Images
Western blot - HRP Anti-NDUFV2 antibody [EPR15351(B)] (AB209040)
  • WB

Lab

Western blot - HRP Anti-NDUFV2 antibody [EPR15351(B)] (AB209040)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209040 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-NDUFV2 antibody [EPR15351(B)] (ab209040) at 1/5000 dilution

All lanes:

Heart (Human) Tissue Lysate - fetal normal tissue at 10 µg

Predicted band size: 27 kDa

Observed band size: 24 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15351(B)

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB183717

Anti-NDUFV2 antibody [EPR15350(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV2 antibody [EPR15350(B)] (AB183717)

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Complementary Products

Proteins & Peptides

AB171712

Recombinant Human NDUFV2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human NDUFV2 protein (His tag N-Terminus) (AB171712)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFV2 also known as NADH dehydrogenase [ubiquinone] flavoprotein 2 functions as a core subunit of complex I in the mitochondrial electron transport chain. This protein has a mass of approximately 24 kDa and plays a pivotal role in electron transfer by linking flavin mononucleotide and iron-sulfur centers. It is expressed in various tissues but shows high levels in cells with elevated energy demands such as muscle and brain tissues.
Biological function summary

NDUFV2 contributes to energy production in the cell by facilitating the transfer of electrons from NADH to the ubiquinone pool. As part of complex I which is the first enzyme complex in the respiratory electron transport chain it helps in establishing the proton gradient across the inner mitochondrial membrane. The activity of NDUFV2 is essential for ATP synthesis and its proper function ensures efficient cellular respiration.

Pathways

NDUFV2 operates in the oxidative phosphorylation pathway an important energy production route. This protein is intricately linked with other complex I subunits including NDUFS1 which work together to initiate the process of oxidative phosphorylation. Another key component in this pathway is ubiquinone which serves as the electron transfer intermediary continuing the electron passage through the chain.

Mutations in NDUFV2 have associations with neurodegenerative diseases and cardiac dysfunction. For instance patients with Leber Hereditary Optic Neuropathy often present alterations in complex I that involve NDUFV2. Additionally disruptions in its function can influence the onset of conditions such as cardiomyopathy where complex I perturbation including proteins like NDUFS1 can compromise mitochondrial energy production leading to heart muscle inefficiency.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (Probable). Parts of the peripheral arm of the enzyme, where the electrons from NADH are accepted by flavin mononucleotide (FMN) and then passed along a chain of iron-sulfur clusters by electron tunnelling to the final acceptor ubiquinone (Probable). Contains one iron-sulfur cluster (Probable).
See full target information NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 23: PubMed33300072

2020

miR‑654‑3p suppresses cell viability and promotes apoptosis by targeting RASAL2 in non‑small‑cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xiong,Shigang Xing,Zheng Dong,Lei Niu,Qinghua Xu,Yusheng Li,Pingyi Liu,Peixia Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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