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AB201773

HRP Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717]

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(1 Publication)

Rabbit Recombinant Monoclonal Niemann Pick C1 Like 1/NPC1L1 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NPC1-like intracellular cholesterol transporter 1, NPC1L1, Niemann-Pick C1-like protein 1

1 Images
Western blot - HRP Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (AB201773)
  • WB

Lab

Western blot - HRP Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (AB201773)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201773 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab201773) at 1/5000 dilution

All lanes:

Liver (Human) Tissue Lysate - fetal normal tissue at 10 µg

Predicted band size: 149 kDa

Observed band size: 149 kDa

true

Exposure time: 12min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5717

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Niemann Pick C1 Like 1 (NPC1L1) also known simply as NPC1L1 is a protein that plays a critical role in the intestinal absorption of dietary cholesterol. NPC1L1 is a multi-pass membrane protein located in the brush border of small intestine enterocytes and also present in the liver. It consists of approximately 1334 amino acids giving it a mass of around 148 kDa. This protein serves as a conduit for cholesterol as it moves across the cell membrane taking part in the cholesterol uptake mechanism.
Biological function summary

NPC1L1 is essential for maintaining cholesterol homeostasis within the body. It is not part of a larger protein complex but works closely with other proteins to facilitate lipid incorporation into chylomicrons. Inhibitors of NPC1L1 such as ezetimibe effectively reduce blood cholesterol levels by blocking this absorption route. The presence of NPC1L1 on the apical surface of enterocytes emphasizes its function in the body's cholesterol metabolism impacting systemic lipid levels.

Pathways

NPC1L1 is involved with cholesterol absorption pathways an important component of lipid homeostasis. This pathway involves other important proteins such as the Niemann-Pick C1 protein (NPC1) and ATP-binding cassette transporters like ABCG5 and ABCG8. These proteins along with NPC1L1 coordinate the regulation of cholesterol levels by balancing absorption and excretion aiding in maintaining overall homeostasis in the body.

NPC1L1's role in cholesterol regulation links it directly to atherosclerosis and hypercholesterolemia. Elevated levels of cholesterol can lead to plaque formation in arteries contributing to coronary artery disease. Ezetimibe a drug targeting NPC1L1 demonstrates this connection as it helps lower cholesterol levels by inhibiting NPC1L1 function. Moreover interactions with other proteins like APOB (Apolipoprotein B) and LDL receptor exemplify the broader implication of NPC1L1 in lipid metabolism disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a major role in cholesterol homeostasis (PubMed : 22095670). Critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte (PubMed : 22095670). Involved in plant sterol absorption, it transports sitosterol, although at lower rates than cholesterol (By similarity). Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorption and is approved for the treatment of hypercholesterolemia (PubMed : 15928087). May have a function in the transport of multiple lipids and their homeostasis, thereby influencing lipid metabolism regulation (PubMed : 15671032). May be involved in caveolin trafficking from the plasma membrane (By similarity). In addition, acts as a negative regulator of NPC2 and down-regulates its expression and secretion by inhibiting its maturation and accelerating its degradation (PubMed : 22095670).
See full target information NPC1-like intracellular cholesterol transporter 1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 9:556-563 PubMed29483961

2018

The prognostic value of Niemann-Pick C1-like protein 1 and Niemann-Pick disease type C2 in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ke-Ji Chen,Ri-Ming Jin,Chun-Chao Shi,Rui-Liang Ge,Lei Hu,Qi-Fei Zou,Quan-Yu Cai,Guang-Zhi Jin,Kui Wang
View all publications
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